Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Luminex"”.
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Development and utilization of Luminex biomarker assays for diagnosis and monitoring of neurodegenerative disease
… has been described. This assay system uses the Luminex technology and has been developed to quantitatively measure phosphorylated α-synuclein, total α-synuclein, total DJ-1 and LRRK2 in human CSF and plasma. These proteins are predominantly implicated in diseases collectively termed …
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Luminex Microsphere Immunoassay Offers an Improved Method in Testing for Antibodies to Eastern Equine Encephalitis Virus in Sentinel Chicken Sera
Eastern Equine Encephalitis virus has a human mortality rate of 30% of those cases diagnosed, while 30% of those surviving infection remain with neurological sequelae for life (CDC.gov, 2007). The use of sentinel chickens for surveillance of arboviruses that are known to use birds as a reservoir …
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Peripheral inflammatory and regulatory immune changes in HIV positive to HIV positive renal transplant recipients
… the blood circulation. METHODOLOGY A multiplexed Luminex assay was used to measure the concentrations of 67 inflammatory and regulatory plasma cytokines immediately pre-transplant, at 1, 3, 6 and 12 weeks PT. Two separate manufacturers of Luminex panels were used and a series of statistical …
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DIABETES MELLITUS AND THE DYSREGULATION OF ANGIOGENESIS IN TUBERCULOSIS
… viability. Following stimulation, a multiplex Luminex assay revealed early induction of angiogenic factors in both healthy and DM groups, with a trend toward reduced secretion in DM. scRNA-seq identified angiogenic gene signatures in select myeloid populations. In summary, this study provides …
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Describing the immune cell profile of ventricular cerebrospinal fluid (CSF) in paediatric central nervous system (CNS) infections
… CNS infections through flow cytometric and Luminex® analysis respectively, and 2) explore changes in immune cells and analytes over time. Methods: CSF samples were prospectively collected during clinically indicated procedures, the cell pellets and supernatant were cryopreserved. Flow …
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Exposure of 3D organotypic vaginal tissues to semen induces expression of the chemokine MCP-1, but does not alter tissue integrity
… the upregulation of inflammatory cytokines by Luminex assay and microarray technology, and tissue integrity by histology and transepithelial electrical resistance (TEER) assessments. We used three multilayered differentiated EpiVaginal™ models: VEC-FT, which contained both epithelium and …
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Investigation du rôle de pikfyve dans la progression tumorale des carcinomes rénaux
… lentiviral inductible shRNA. Un test multiplex luminex a été réalisé afin d'analyser la concentration des facteurs de croissance, de cytokines et des métalloprotéinases matricielles (MMPs) présentent dans le milieu conditionné récolté des cellules exprimant le shRNA. De plus, les cellules 786-0 …
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The impact of sexually transmitted infections and inflammation in the female genital tract and blood on susceptibility to HIV-1 infection and disease progression
… (CVL) and plasma samples from these women using Luminex and ELISA.
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Characterisation of cytokine secretion in malignant gliomas
… included. The analysis was completed utilising a luminex immunoassay. This technology is able to measure 34 cancer-associated analytes simultaneously. Immunohistochemistry of candidate biomarkers was done using primary tumour tissue. The results show that there were significant differences in …
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Development of a Fluorescence Microsphere Immunoassay for Detection of PRRSV Infection Using Oral Fluid Samples as an Alternative to Serum-based Assays
… were used as antigen and covalently coupled to Luminex fluorescent microspheres. Based on an evaluation of 488 oral fluid samples with known serostatus, the oral fluidbased FMIAs achieved greater than 92% sensitivity and 91% specificity. In serum samples (n = 1639), the FMIAs reached greater …
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The Association Between Cancer-Related-Fatigue, Response to Fatigue Treatment, and Cytokines and Their Receptors, In Patients With Advanced Cancer
… and R2, IL-8, IL-10, IL-17] levels measured by Luminex. Data were analyzed using principal component analysis (PCA) [reporting cumulative variance (variance) for the first four components] to determine their association with fatigue and response to treatment. <br …
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Human papillomavirus testing and its application in cervical cancer prevention
… to fluorescent beads for detection on the Luminex platform. The utility of HPV-testing was evaluated in 3 different studies: A general primer PCR-based genotyping method and the commercial Hybrid Capture (HCII) assay were compared for sensitivity and specificity for detection of CIN in …
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Reabilitação em curto período com PRGF de segunda geração geração associada a implantes: estudo clínico randomizado
… a partir do PRGF-2 obtido utilizando o Luminex®. Sessenta e nove participantes de pesquisa foram incluídos no estudo, divididos aleatoriamente em Grupo 1 (35 implantes, controle) e Grupo 2 (34 implantes com PRGF-2, grupo experimental). Observou-se que, a partir de 27 proteínas avaliadas, …
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Identification of Novel Fluid Biomarkers for Alzheimer's Disease
… for levels of 190 analytes using a multiplexed Luminex platform. The mean concentrations of 37 analytes were found to differ between CDR 0 and CDR>0 participants. ROC and statistical machine learning algorithms identified novel biomarker panels that improved upon the ability of the current best …
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Development and Evaluation of a Multiplex Suspension Array Protocol for the Detection of Enteric Pathogens from Clinical Specimens
… outbreak etiologic agents. One technology is the Luminex XMap® microsphere system, which is also marketed as the Bio-Plex™ 200. This platform has a multiplexing capability with the potential to simultaneously detect up to 100 targets in one reaction. The studies described here show that the …
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The Impact of a Scleral Lens on the Eye
… molecules were quantitated using Luminex multiplex immunoassay and compared between tear sample types. (2) In normal subjects (n=13) wearing SL, optical coherence tomography (OCT) was used to quantitate MDF, which was then correlated to relative lipid and protein abundances (mass …
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The influence of the hormonal milieu on eicosanoid and cytokine production in tissues from the female reproductive tract.
… Enzyme-Linked Immunosorbent Assay (ELISA) or Luminex®. Human endometrium produced greater concentrations of TXB2, PGE2 and PGF2¿ than myometrium in vitro (p<0.05). Fifteen prostanoids were detected in human myometrium. Production of 6-keto-PGF1¿, PGE1 and PGF1¿ increased whilst 15- keto PGE2 …
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Characterisation of Alloantibody-Human Leukocyte Antigen Interactions to Improve Immunological Risk Assessment in Solid Organ Transplantation
… that govern their pathogenic potential. Luminex single antigen beads (SABs), SAB-C1q, flow cytometry (FC) and complement dependent cytotoxicity (CDC) assays were used to determine the reactivity and complement fixing capacities of human monoclonal antibodies, whilst biolayer …
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Biomarkers of neurological tissue injury and inflammation in paediatric tuberculous meningitis
… glial fibrillary acidic protein (GFAP), and with Luminex multianalyte array assay for a panel of inflammatory markers. Results were compared with 2 controls groups. Computerized tomography was done on admission and magnetic resonance imaging (brain, spine and magnetic resonance angiography) at 3 …
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DETERMINANTS OF HIGH-RISK HPV SEROPREVALENCE AND DNA PREVALENCE IN MID-ADULT WOMEN
… antibodies to the same HPV types using a Luminex-based assay. Health and sexual history were obtained via self-reported questionnaires. Risk factors for seropositivity to hr HPV, and hr HPV DNA-positivity were assessed using Poisson regression models to obtain prevalence ratios (PRs). …
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