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Showing 1 to 10 of 10 for “"Luminescence Resonance Energy Transfer"”.
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Luminescence Resonance Energy Transfer Studies of the Shaker Potassium Voltage-Gated Ion Channel
… sensing. We use a technique called Lanthanide Resonance Energy Transfer (LRET), which gives an accurate distance measurement between two positions on the ion channel. We use two different configurations for LRET on Shaker. The first measures distances between the four identical S4 amino-acid …
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Luminescence resonance energy transfer studies of the Shaker K+ voltage-gated ion channel
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-26T23:06:21Z No. of bitstreams: 1 Posson_David.pdf: 7844031 bytes, checksum: 3cee2902577cf067066066ebcaad3557 (MD5)
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Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit
A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… substituted cysteine accessibility method and luminescence resonance energy transfer. Using the latter methodology in particular, as well as a new method to produce purified hemichannels of controlled subunit composition, I was able to assign all helices in the available low-resolution …
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Investigations of The Structure-Function Relationship In Kainate Receptors Using FöRster Resonance Energy Transfer
… limited. The work in this dissertation utilizes luminescence resonance energy transfer (LRET) and single-molecule Förster Resonance Energy Transfer (smFRET) in order to address this gap in the knowledge. We have characterized the structural arrangement and dynamics of the homomeric (GluK2) …
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The Structural Mechanism of Allosteric Modulation of The Nmda Receptor: A Balance of Tensions
… the ATDs and inhibits the receptor. We used luminescence resonance energy transfer to study the structural changes accompanying modulator binding and then linked those conformational changes to the function of the receptor using electrophysiology. LRET measurements were made between subunits …
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Ampa Receptor Allosterism: Measurement of The Conformational Changes In The Ligand Binding Domain of A Functional Receptor
… state. In this dissertation, a technique called Luminescence Resonance Energy Transfer was used to determine the conformational changes associated with activation and desensitization in a functional AMPA receptor (ÄN*-AMPA) that contains the ligand binding domain and transmembrane segments; …
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Digital Profiling of Circulating Extracellular Vesicles at Single-Upconversion Nanoparticle Sensitivity and Resolution
… assay (ELISA). Compared with previous luminescence resonance energy transfer (LRET) method using UCNPs for detection of EVs, our technique achieves single tumour-derived sEV quantification. In chapter 3, we report super-resolution imaging technique for single CD9+EpCAM+EV analysis. The …
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Conformational Changes In The Extracellular Domain of Glutamate Receptors
… channel opening, I have used a technique called Luminescence Resonance Energy Transfer (LRET). LRET offers the unique advantage of tracking large scale conformational changes associated with receptor activation and desensitization. In this dissertation, LRET, in combination with biochemical and …
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Novel peptide-based approaches to study the activity and substrate specificity of protein kinases
… the phosphotyrosine, leading to an increase in luminescence and thus providing a direct measure of kinase activity. To overcome the problem of competitive binding by ATP, however, it was found to be necessary to add a large excess of the complex, which led to a high degree of enzyme “poisoning” …