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Showing 1 to 20 of 33 for “"Loop-mediated isothermal amplification (LAMP)"”.

  1. Loop-mediated isothermal amplification (LAMP) for the diagnosis of human sleeping sickness: towards a point-of-care diagnostic test

    Wastling, Sally LouiseThe University of Edinburgh2011

    … reservoirs of infection. This study focuses upon LAMP (loop-mediated isothermal amplification) as a novel diagnostic for sleeping sickness that may serve to bridge the gap between the need for sensitive, specific molecular diagnostics on the one hand and ‘field-friendly’ diagnostics on the other. …

    edinburgh Repository record

  2. Development of a Gram-type Specific Method for Detection of Bovine Mastitis Pathogens by Combining Loop-mediated Isothermal Amplification (LAMP) and Split Trehalase Technologies

    Miao, ZhuohanVeterinary Medicine2022

    … prescribe through two steps: (i) develop PCR and LAMP assay for a point-of-care (POC) amplification of Gram-positive and Gram-negative bacteria and (ii) use a modified amplicon binding split trehalase assay (ABSTA) to detect this amplification by a specific protein-DNA binding and the …

    calgary Repository record

  3. Development and Use of Loop-Mediated Isothermal Amplification (LAMP) Assays to Detect the Presence of Pathogens Which Cause Urinary Tract Infection and Genes Which Cause Resistance to 3rd Generation Cephalosporins

    Diggle, Joanna Morfydd2025

    … bacterial uropathogens direct from urine, using loop-mediated isothermal <br/>amplification (LAMP) technology. Then, to assess whether the same technology could be adapted to <br/>detect bacterial AMR genes, with the potential to direct treatment decisions and improve current <br/>methods of UTI …

    southwales Repository record

  4. Prevalence and risk factors associated with Trypanosomosis in Cattle Herds in Kilwa District of Lindi Region of Tanzania

    Kassian, Nicholas EmmanuelUniversity of Zambia2015

    … and pooled sample at herd level were analysed by loop mediated isothermal amplification (LAMP). A herd was considered positive if at least one animal in the herd was positive for AAT. A herd prevalence of 9.3 % (95% CI: 2.9-14.9) was recorded for AAT by microscopy, mainly caused by Trypanosoma …

    zimbabwe Repository record

  5. Prevalence and risk factors associated with Trypanosomosis in Cattle Herds in Kilwa District of Lindi Region of Tanzania

    Kassian, Nicholas EmmanuelUniversity of Zambia2015

    … and pooled sample at herd level were analysed by loop mediated isothermal amplification (LAMP). A herd was considered positive if at least one animal in the herd was positive for AAT. A herd prevalence of 9.3 % (95% CI: 2.9-14.9) was recorded for AAT by microscopy, mainly caused by Trypanosoma …

    zambia Repository record

  6. ST3GAL3 GENE MUTATION WITHIN THE SINGULAR NUCLEOTIDE POLYMORPHISM, RS3952787, POTENTIALLY COULD PREDICT ATTENTION DEFICIT HYPERACTIVE DISORDER, ANXIETY, AND DEPRESSION

    Balinski, Rebecca R2030

    … disorders are commonly diagnosed alongside ADHD. Loop-mediated isothermal amplification (LAMP) will be the primary assay utilized in this study.</p>

    nmu Repository record

  7. Genetic Alterations Associated with Attention Deficit Hyperactivity Disorder

    Thomas, Nicole2028

    … component underlying ADHD. This study uses Loop Mediated Isothermal Amplification (LAMP) to amplify the wild type of the ST3GAL3 gene in two groups of participants: one displaying ADHD characteristics as defined by the Adult ADHD Self-Report Scale (ASRS-v1.1) Symptom Checklist, and one that …

    nmu Repository record

  8. Desarrollo de un método de detección sensible y específico de Pseudomonas syringae pv. syringae y Pseudomonas syringae pv. morsprunorum en cerezos

    Díaz Leal, Daniela PazUniversidad de Chile2022

    … basado en la técnica molecular denominada Loop-mediated isothermal amplification (LAMP). A partir de la secuencia del genoma de aislados bacterianos obtenidos de material vegetal de cerezo sintomático, colectados en cuatro regiones del país, en conjunto con secuencias disponibles de …

    chile Repository record

  9. EXPLORING THE CORRELATION OF THE PRESENCE OF THE ST3GAL3 GENE IN INDIVIDUALS DIAGNOSED WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH COLORIMETRIC DETECTION

    Nelson, Rachael2027

    … individuals.. In order to test this hypothesis, Loop Mediated Isothermal Amplification (LAMP) was used for rapid amplification of the wild type ST3GAL3 gene target. DNA samples were collected from 80 participants, 40 with a self-reported positive ADHD diagnosis, 40 with no self-reported ADHD …

    nmu Repository record

  10. THE ROLE OF MTHFR GENE MUTATION IN NEURODEVELOPMENTAL DISORDERS: EXPLORING POTENTIAL CORRELATIONS WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER AND ANXIETY

    Mehmood, Heba2025

    … to assess their signs and symptoms of ADHD. DNA amplification of the MTHFR gene was achieved using loop-mediated isothermal amplification (LAMP), with colorimetric visualization to determine the presence of mutations. Results were analyzed using Fisher’s exact tests to explore the associations …

    nmu Repository record

  11. Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup

    Ornob, AkidUniversity of Illinois at Urbana-Champaign2018

    … approximately 30 minutes. Our approach utilizes loop mediated isothermal amplification (LAMP) reagents pre-deposited into distinct lanes of the microfluidic chip, which, when exposed to target nucleic acid sequences from the test sample, generates fluorescent products that, when excited by …

    uiuc Repository record

  12. Nanobiotechnology Enabled Environmental Sensing of Water and Wastewater

    Kang, SejuVirginia Tech2023

    … in regions with poor WASH services. First, loop-mediated isothermal amplification (LAMP) and nanopore sequencing were applied to develop assays for the detection of SARS-CoV-2, the causative agent of COVID-19, in wastewater samples. Second, surface-enhanced Raman spectroscopy (SERS) was …

    vt Repository record

  13. DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM

    Brown, William2020

    … This study describes the development of a novel loop-mediated isothermal amplification (LAMP) reaction followed by a peptide nucleic acid (PNA) probe and gold nanoparticle (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, …

    nmu Repository record

  14. Sensitivity of a LAMP Assay for Detection of the Dinoflagellate Amyloodinium ocellatum in Simulated Field Conditions and Freeze Tolerance of the Parasite

    Gonzales, Robert2022

    … for control. In this work, microscopy and a loop-mediated isothermal amplification (LAMP) assay were compared for early diagnosis of <em>A. ocellatum</em> in cultured stocks, and the freeze tolerance of tomonts was assessed to determine if frozen wild fish used as fish food can serve as a …

    usm Repository record

  15. DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION

    Peacock, Tess2024

    … </em>species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of <em>Legionella </em>in less than one hour. LAMP utilizes four primers, a strand displacing DNA polymerase, and a single incubation temperature to amplify DNA …

    nmu Repository record

  16. Novel and newer nucleic acid amplification tests for the diagnosis of TB

    Matinyenya, BrianDivision of Pulmonology2016

    … the diagnostic accuracy of the promising novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South …

    cape-town Repository record

  17. Single-Laboratory Validation for the Detection of <em>Salmonella enterica</em> in Meat Analog Products

    Tabb, Amanda2025

    … alternatives to VIDAS are real-time PCR or loop-mediated isothermal amplification (LAMP). The objective of this study was to optimize the procedures for pre-enrichment and screening of meat analogs for the detection of Salmonella. This was accomplished by inoculating meat analog samples with …

    chapman Repository record

  18. Silaffin Tagged Polymerase for Nucleic Acid Amplification

    Seevaratnam, DushanthUniversity of Cambridge2020

    … and compatible with sensitive and specific loop mediated isothermal amplification (LAMP) assays. Fusing the enzyme with a silaffin tag (R5) and a fluorescent protein (mCherry) enabled rapid and cost-effective purification through unmodified silica immobilisation and built-in visual tracking …

    cambridge Repository record

  19. RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION

    Nelson, Rachael2025

    … coli</em>.<em> </em>This research utilizes a DNA amplification technique called Loop-Mediated Isothermal Amplification (LAMP) to detect different strains of pathogenic <em>E. coli</em>. Common visualization methods of LAMP amplicons include using LAMP reaction buffer containing a pH indictor dye. …

    nmu Repository record

  20. Equine Herpesvirus Type 1: Filling Gaps Toward Improved Outbreak Management

    Saklou, Nadia TalalVirginia Tech2023

    … for improving the management of EHV-1 disease. Loop mediated isothermal amplification (LAMP) is a method that amplifies nucleic acid rapidly at a constant temperature and is minimally affected by inhibitors that are often found in clinical samples. This procedure can be followed by multiple …

    vt Repository record

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