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Showing 1 to 20 of 46 for “"Loop mediated isothermal amplification"”.

  1. On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification

    Duarte Guevara, CarlosUniversity of Illinois at Urbana-Champaign2013

    … device capable of performing nucleic acid amplification will enable effective detection of infectious agents in multiple settings, allowing better enforcement of food safety regulations. Current protocols and practices for control of agents responsible for foodborne disease will become …

    uiuc Repository record

  2. An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.

    Sandala, Golden SeuluThe University of Zambia2023

    … was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering various rabies virus …

    zimbabwe Repository record

  3. An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.

    Sandala, Golden SeuluThe University of Zambia2023

    … was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering various rabies virus …

    zambia Repository record

  4. Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone

    Sun, FuUniversity of Illinois at Urbana-Champaign2018

    New tools are needed to enable rapid detection, identification, and reporting of infectious pathogens in a wide variety of point-of-care applications that impact human and animal health. In this work, we report the design, construction, and characterization of a platform for multiplexed analysis of …

    uiuc Repository record

  5. Loop mediated isothermal amplification based detection of equine respiratory pathogens using a portable, smartphone-based setup

    Ornob, AkidUniversity of Illinois at Urbana-Champaign2018

    … approximately 30 minutes. Our approach utilizes loop mediated isothermal amplification (LAMP) reagents pre-deposited into distinct lanes of the microfluidic chip, which, when exposed to target nucleic acid sequences from the test sample, generates fluorescent products that, when excited by …

    uiuc Repository record

  6. SENSITIVE AND SPECIFIC DETECTION OF SARS-COV-2 IN SALIVA USING REVERSE TRANSCRIPTASE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

    Juntila, Casey2021

    <p>A novel coronavirus called SARS-CoV-2 has caused the emergency release of the most well-known molecular assay, the CDC N1 RT-PCR assay, causing reports of poor analytical performance resulting in false-negative results (20, 26), and inconsistent testing kits received (23, 32). An immediate and …

    nmu Repository record

  7. DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION

    Peacock, Tess2024

    … </em>species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of <em>Legionella </em>in less than one hour. LAMP utilizes four primers, a strand displacing DNA polymerase, and a single incubation temperature to amplify DNA …

    nmu Repository record

  8. Loop-mediated isothermal amplification (LAMP) for the diagnosis of human sleeping sickness: towards a point-of-care diagnostic test

    Wastling, Sally LouiseThe University of Edinburgh2011

    … of infection. This study focuses upon LAMP (loop-mediated isothermal amplification) as a novel diagnostic for sleeping sickness that may serve to bridge the gap between the need for sensitive, specific molecular diagnostics on the one hand and ‘field-friendly’ diagnostics on the other. Here, …

    edinburgh Repository record

  9. A Rapid Colorimetric Peptide Nucleic Acid Loop-Mediated Isothermal Amplification Assay for the Detection of the IDH1 Mutation in Glioblastoma

    Raack, Edward J2017

    … a novel colorimetric peptide nucleic acid loop-mediated isothermal amplification (PNA-LAMP) method that rapidly detects the <em>IDH1</em> R132H mutation in GBM. PNA-LAMP amplifies target DNA under isothermal conditions with high specificity and speed. The PNA prevents amplification of …

    nmu Repository record

  10. Development of a Gram-type Specific Method for Detection of Bovine Mastitis Pathogens by Combining Loop-mediated Isothermal Amplification (LAMP) and Split Trehalase Technologies

    Miao, ZhuohanVeterinary Medicine2022

    … PCR and LAMP assay for a point-of-care (POC) amplification of Gram-positive and Gram-negative bacteria and (ii) use a modified amplicon binding split trehalase assay (ABSTA) to detect this amplification by a specific protein-DNA binding and the complementation of split trehalase. To do this, …

    calgary Repository record

  11. EXPLORING THE CORRELATION OF THE PRESENCE OF THE ST3GAL3 GENE IN INDIVIDUALS DIAGNOSED WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH COLORIMETRIC DETECTION

    Nelson, Rachael2027

    … individuals.. In order to test this hypothesis, Loop Mediated Isothermal Amplification (LAMP) was used for rapid amplification of the wild type ST3GAL3 gene target. DNA samples were collected from 80 participants, 40 with a self-reported positive ADHD diagnosis, 40 with no self-reported ADHD …

    nmu Repository record

  12. Förster Resonance Energy Transfer-Based Quantification of D-2-Hydroxyglutarate & Locked Nucleic Acid Loop-Mediated Isothermal Amplification Mediated Detection of IDH1-R132 Single Nucleotide Variants in Glioma

    Choate, Kristian A2029

    <p>Isocitrate dehydrogenase 1 (<em>IDH1</em>) is a biomarker in gliomas which provides valuable information on patient prognosis and treatment. Targeted therapies including maximal surgical resection and pharmaceutical mutant IDH inhibitors offer life extending benefits; however, currently …

    nmu Repository record

  13. RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION

    Nelson, Rachael2025

    … coli</em>.<em> </em>This research utilizes a DNA amplification technique called Loop-Mediated Isothermal Amplification (LAMP) to detect different strains of pathogenic <em>E. coli</em>. Common visualization methods of LAMP amplicons include using LAMP reaction buffer containing a pH indictor dye. …

    nmu Repository record

  14. The application of loop mediated isothermal amplification for the detection of the sexually transmitted pathogens Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis, at the point of care.

    Edwards, TRLiverpool John Moores University2014

    … operated microfluidic disk (the LabDisk), with loop mediated isothermal amplification (LAMP) and optical detection, capable of detecting the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium and Trichomonas vaginalis in clinical urine and swab …

    liverpool-jm Repository record

  15. Development and Use of Loop-Mediated Isothermal Amplification (LAMP) Assays to Detect the Presence of Pathogens Which Cause Urinary Tract Infection and Genes Which Cause Resistance to 3rd Generation Cephalosporins

    Diggle, Joanna Morfydd2025

    … bacterial uropathogens direct from urine, using loop-mediated isothermal <br/>amplification (LAMP) technology. Then, to assess whether the same technology could be adapted to <br/>detect bacterial AMR genes, with the potential to direct treatment decisions and improve current <br/>methods of UTI …

    southwales Repository record

  16. DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM

    Brown, William2020

    … This study describes the development of a novel loop-mediated isothermal amplification (LAMP) reaction followed by a peptide nucleic acid (PNA) probe and gold nanoparticle (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, …

    nmu Repository record

  17. Prevalence and risk factors associated with Trypanosomosis in Cattle Herds in Kilwa District of Lindi Region of Tanzania

    Kassian, Nicholas EmmanuelUniversity of Zambia2015

    … and pooled sample at herd level were analysed by loop mediated isothermal amplification (LAMP). A herd was considered positive if at least one animal in the herd was positive for AAT. A herd prevalence of 9.3 % (95% CI: 2.9-14.9) was recorded for AAT by microscopy, mainly caused by Trypanosoma …

    zimbabwe Repository record

  18. Prevalence and risk factors associated with Trypanosomosis in Cattle Herds in Kilwa District of Lindi Region of Tanzania

    Kassian, Nicholas EmmanuelUniversity of Zambia2015

    … and pooled sample at herd level were analysed by loop mediated isothermal amplification (LAMP). A herd was considered positive if at least one animal in the herd was positive for AAT. A herd prevalence of 9.3 % (95% CI: 2.9-14.9) was recorded for AAT by microscopy, mainly caused by Trypanosoma …

    zambia Repository record

  19. ST3GAL3 GENE MUTATION WITHIN THE SINGULAR NUCLEOTIDE POLYMORPHISM, RS3952787, POTENTIALLY COULD PREDICT ATTENTION DEFICIT HYPERACTIVE DISORDER, ANXIETY, AND DEPRESSION

    Balinski, Rebecca R2030

    … disorders are commonly diagnosed alongside ADHD. Loop-mediated isothermal amplification (LAMP) will be the primary assay utilized in this study.</p>

    nmu Repository record

  20. Genetic Alterations Associated with Attention Deficit Hyperactivity Disorder

    Thomas, Nicole2028

    … component underlying ADHD. This study uses Loop Mediated Isothermal Amplification (LAMP) to amplify the wild type of the ST3GAL3 gene in two groups of participants: one displaying ADHD characteristics as defined by the Adult ADHD Self-Report Scale (ASRS-v1.1) Symptom Checklist, and one that …

    nmu Repository record

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