Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Locked nucleic acid"”.
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Developing in vitro models to elucidate the mechanisms of toxicity of locked nucleic acid gapmer antisense oligonucleotides.
Antisense oligonucleotides are an emerging class of RNA therapeutics that have recently received several approvals from the clinic. Antisense oligonucleotides act at the RNA level to either degrade target RNA, or to regulate splicing of a particular gene, and have been applied to treat a variety of …
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Förster Resonance Energy Transfer-Based Quantification of D-2-Hydroxyglutarate & Locked Nucleic Acid Loop-Mediated Isothermal Amplification Mediated Detection of IDH1-R132 Single Nucleotide Variants in Glioma
<p>Isocitrate dehydrogenase 1 (<em>IDH1</em>) is a biomarker in gliomas which provides valuable information on patient prognosis and treatment. Targeted therapies including maximal surgical resection and pharmaceutical mutant IDH inhibitors offer life extending benefits; however, currently …
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Nanoparticle probes for the study of biomolecular interactions
… The oligonucleotides are modified to incorporate locked nucleic acid (LNA) ribose sugars, affording highly advantageous affinity and selectivity properties. Hybridisation of the probes with the target sequence was monitored colorimetrically and by extinction spectroscopy. Double stranded DNA was …
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DNA based biosensors for environmental and medical applications
… reaction. Among the sequence probes, modified locked nucleic acid (LNA) and the peptide nucleic acid (PNA) were used. In this case the screen printed electrode Abstract iv was used only as transducer while the hybridization assay was conducted onto paramagnetic micro beads. The genosensor was …
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Direct Quantitative Analysis of Multiple Micrornas
… the specificity of DQAMmiR I incorporated locked nucleic acid (LNA) bases into the probes to normalize the melting temperature of all target miRNA hybrids. This allowed me to use a single hybridization temperature, at which all target miRNA hybrids remained intact while single-nucleotide …
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Targeting oncogenic microRNAs in malignant germ cell tumours.
… transcript (pri-miRNA) of each cluster using locked nucleic acid (LNA)-based gapmeRs and targeting the mature miRNAs with peptide nucleic acid and LNA-based inhibitors. Targeting the mature microRNAs using LNA-based inhibitors resulted in a significant and reproducible negative growth …
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Detection of food contaminants by SERS and nanopore technology
… were mixed with DNA probes (with or without a locked nucleic acid (LNA) substitution) to form double-strand duplexes. Results show single LNA substitution of the probe can efficiently discriminate E. coli O157:H7 and others by magnifying the ratio of durations of the events from 1.6 to 12.2 …
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Development of therapeutic agents for myotonic dystrophy type 1
… the oligomer can form polyplexes with a locked nucleic acid (LNA) CAG16 gapmer, facilitating its cell uptake in both HeLa cells and DM1 patient myotubes, with a resulting decrease in the level of toxic r(CUG)exp significantly. RNA-seq analysis indicated that the oligomeric ligand and its …
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The role of long non-coding RNA LINC00673 in lung tumorigenesis
… at a potential function in cis. Interestingly, locked nucleic acid antisense oligonucleotide (LNA ASO) knockdown of LINC00673 enhances cell growth contrary to our hypothesis that the lncRNA functions as an oncogene. Knock-down of LINC00673 by CRISPRi and LNA ASOs suggest that downregulation of …
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Effects of survivin and survivin inhibition in canine models of lymphoma and osteosarcoma
… Next we inhibited survivin using EZN-3042, a locked nucleic acid oligonucleotide targeting survivin, in two canine LSA and two canine OS cell lines. Survivin inhibition was confirmed by qRT-PCR and Immunofluorescence. Percent dead and total cell number were assessed by manual cell counting …
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LNA-clamp-PCR zum sensitiven Nachweis von Punktmutationen im Rahmen der Entwicklung eines Darmkrebsfrüherkennungstests
… Arbeit wurde dazu die Methode der LNA-clamp-PCR (locked nucleic acid) etabliert. Die Bewertung der erzielten Anreicherung erfolgte über das relative Detektionslimit. Zur Bestimmung des Detektionslimits wurde die Schmelzkurvenanalyse von Hybridisierungssonden eingesetzt; diese wurde im Rahmen …