Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Localization signal"”.
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… amino acid motif nor the responsible nuclear localization signal (NLS) has not been previously reported. Here, we report that two histidine residues separated by a 10-amino-acid spacer (H1068-H1079) located in the juxtamembrane region of c-Met function as a putative novel NLS. …
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Recognition Mechanisms of Nuclear Localization and Export Signals
… their substrates through either a nuclear localization or export signal depending on the direction of transport. Even though there are ten yeast import Karyopherin betas, for the past thirteen years there was only one well characterized nuclear localization signal, the classical nuclear …
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Functional Study of Hemogen Knockout Mouse Model
… a deletion mutant of the predicted nuclear localization signal, I conclude the amino acids 61 to 78 is a functional nuclear localization signal. Both the nuclear localization signal and the predicted coiled-coil domain were required for Hemgn to protect Ba/F3 cells from IL-3 withdrawal …
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Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
… phosphorylation, is responsible for the nuclear localization signal.
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Mapping of the repression domain and the subcellular localization of the ABF-1 protein
… To complement these studies, the subcellular localization and nuclear localization signal of GFP-tagged ABF -1 proteins was performed using fluorescent microscopy. These studies suggest that ABF-1 exerts it function through the bHLH domain and the region of the nuclear localization signal lies …
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Investigation into the Akirin Subcellular Localization and Developmental Interactions
… investigation focused on both the subcellular localization of Akirin based on a putative nuclear localization signal and the genetic interaction between Akirin and the Nucleosome Remodeling Deacetylase (NuRD) complex. Through transfection of <em>Drosophila</em> S2 cells with Akirin mutant …
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Molecular characterization of XvPer1, a novel antioxidant enzyme from the resurrection plant Xerophyta viscosa, and AC3, a LEA-like protein from Arabidopsis thaliana
… sequence also had a putative bipartite nuclear localization signal.
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High Angular Momentum Rydberg Wave Packets
… depend on their energy, leading to periods of localization and delocalization as the components come into and go out of phase with each other. Monitoring survival probability signals in the presence of position dependent probing leads to observation of characteristic oscillations based on the …
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Investigation of Akirin Nuclear Localization and the Molecular Interaction between Akirin and Taf4 in Drosophila
… the functionality of an Akirin Nuclear Localization Signal (NLS). Through calcium phosphate transfection of S2 cells and fluorescent imaging, we were able to compare wild-type full-length Akirin protein fused to the Venus fluorescent protein to full length Akirin::Venus fusion protein …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… levels. ICP27 contains both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other …
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… a chaperone complex that recognizes a nuclear localization signal (NLS), which is a short peptide sequence. Here, we synthesize and purify a fluorescently-labeled importin-a and a positive control for complex formation, which consists of bovine albumin serum (BSA) covalently conjugated to a …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed using an in vitro SUMOylation assay with recombinant TRP47, and native …
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Proteomic Analyses of HIV-1 Infected T-cells and the Functional Characterization of Cyclophilin B During HIV-1 Infection
… must attempt to inhibit infection as well as signal the surrounding environment to the presence of the pathogen. The results of both efforts cause changes in the expression and/or localization of host cell factors including signaling molecules, RNAs, and proteins. In the second study we …
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Characterizing the cargo binding and regulatory function of the tail domain in Ncd motor protein
… were used to identify the presence of a nuclear localization signal (NLS) in the Ncd polypeptide. The nuclear localization of only the GFP fusion proteins containing the Ncd tail sequence indicates that the NLS is contained within the tail domain. Subsequent, experiments performed with GFP fusion …
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ZFP36L3: a Unique Member of the Tristetraprolin Family of RNA-Binding Tandem Zinc Finger Proteins
… deletion mutants, we determined that a nuclear localization signal that resides within the conserved tandem zinc finger domain was functional, although the C-terminal nuclear export sequence was non-functional. We then demonstrated that the unique repeat domain of L3 was responsible for the …
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Innate immune modulation and pathogenic basis for porcine reproductive and respiratory syndrome virus
… PIAS1, and this region overlapped the nuclear localization signal (NLS) of N. A nuclear localization signal (NLS) knock-out mutant N did not activate NF-κB, and this is likely due to the lack of its interaction with PIAS1 in the nucleus, demonstrating the positive correlation between the …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
… recognizing within the cargoes a unique nuclear-localization signal known as the PY-NLS. Several of Kapβ2 cargoes are mutated in neurological diseases. Two such proteins of interest to my research are the Fused in Sarcoma (FUS) and the HNRNPH2 proteins that are mutated in familial ALS and Bain …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
The nuclear localization signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green …
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AY-‐WB phytoplasma manipulations of host and non-‐host leafhopper interactions
… (SAPs) identified, SAP11, carries a nuclear localization signal and accumulates in plant cell nuclei (Bai et al. 2009). SAP11 is shown to reduce production of plant defense hormone jasmonic acid (Sugio et al. 2011). Stable expression of SAP11 and 3 other SAPs in Arabidopsis increase fecundity …
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