Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 338 for “"Living Cells"”.
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Statistical physics of molecular sorting in living cells
L'abstract è presente nell'allegato / the abstract is in the attachment
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Protein folding in living cells and under pressure
… ultra-weak “quinary” interactions play inside living cells is presented in chapter 4, which also includes a review of the most recent literature studying biomolecular dynamics in their native environments. In chapter 5 we study the time-dependence of protein folding inside living cells as …
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Protein folding in crowded environments and living cells
… and extrapolated to explain function in the living cell. In this thesis, we attempt to bridge this divide by performing studies of protein folding in in vitro crowded environments and in living cells. We begin by investigating the thermodynamic and kinetic behavior of the proteins in …
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Synthetic analog feedback control circuits in living cells
Models of biochemical reaction networks in cells are important for advancing our understanding of complex biological systems and for designing functional synthetic biological circuits. However, most models are based on a deterministic digital framework that is largely incompatible with nonlinear …
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A Mathematical Model of Adhesion Interactions between Living Cells
… force-based model of moving and interacting cells that incorporates a realistic description of cell adhesion and applies it to a system of spherical cells. In addition, several results in matrix theory are proved with the end of showing that the equations produced by the model uniquely …
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Observing and quantifying transposable element activity inside living cells
… engineered for tunable expression inside living cells, then tracked in real time using fluorescence microscopy. We show that even simple transposable element systems, such as the bacterial transposon IS608, exhibit spatial and temporal variation across a population. By quantifying these …
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Quantitative imaging of living cells by deep ultraviolet microscopy
… camera to observe mitosis and motility in living cells without causing necrosis, and quantified absorbance at 280 nm and 260 nm together with tryptophan native fluorescence in order to calculate maps of nucleic acid mass, protein mass, and quantum yield in unlabeled cells. We have also …
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Scalable platforms for computation and memory in living cells
Living cells are biological computers - constantly sensing, processing and responding to biological cues they receive over time and space. Devised by evolution, these biological machines are capable of performing many computing and memory operations, some of which are analogous to and some are …
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Studies of fluorescent imaging for mRNA detection in living cells
… focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) mRNA by …
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Measurements and analyses of transposable element activity inside living cells
… element IS608 to quantify its activity in single cells via fluorescence microscopy. The system can reveal single events, cell-to-cell variations, and temporal and environmental variabilities in real time and individual living cells. With this system, we characterize the relationship of the level …
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Biomimetic cytoskeleton assemblies and living cells on micropillar force sensor arrays
… allow the cultivation of fibroblasts, epithelial cells and heart muscle cells on their tops. Cellular traction forces are determined by measuring the bending of the pillar tops during cell movement. A model system for the actin cortex is produced by crosslinking actin filaments on the pillar …
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Biomechanical regulation of epigenetics and chromatin organization in single living cells
… regulate epigenetics in tumor repopulating cells (TRCs), and two, how these forces unfold chromatin and induce gene expression in single living cells. Our findings suggest that mechanical forces play critical roles in regulating nuclear structure and function. Biomechanical cues can …
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Quantitative phase microscopy for the study of electromotility in living cells
The electric activity of living cells is accompanied with changes in their optical and mechanical properties, which arise from the intrinsic biophysics of the cell membrane. These intrinsic changes can be used as an indicator for cell electric activity, but, to our knowledge, the intrinsic signal …
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Biophysical and gene expression change in living cells by force-induced mechanotransduction
… in regulating the physiologic functions of cells and tissues. The importance of engineering principles in studying the biological behavior of cells is no longer in question. Instead, much research is now focused on how mechanical forces are transduced into biochemical activities and …
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Enzyme-based reporters for mapping proteome and imaging proteins in living cells
… this receptor internalizes as an oligomer into cells.
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Directed evolution of TurboID for efficient proximity labeling in living cells and organisms
… all signaling and regulatory processes in cells. Traditional approaches to proteomics employ mass spectrometry (MS) coupled to biochemical fractionation or affinity purification but require cell lysis prior to analysis which often results in false-negatives from missed interactions or …
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Mechanics of living cells: nonlinear viscoelasticity of single fibroblasts and shape instabilities in axons
… the filamentous scaffold which provides cells with mechanical integrity, architecture and contractility. The first part of this report describes single-cell uniaxial stretching experiments performed on fibroblasts. When placed between fibronectin coated microplates, fibroblasts adopt a …
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Engineering outer-membrane electron transfer proteins for modular biotic-abiotic interfacing with living cells
… MtrC offers a modular platform for interfacing living cells with electronic materials, such as electrodes, conductive synthetic biofilms, and redox enzymes for biosensing or biocatalysis. Furthermore, I demonstrate that the N-terminus of MtrA can be modified without disrupting EET. Fusion of a …
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Quantitative Fluorescence Studies in Living Cells: Extending Fluorescence Fluctuation Spectroscopy to Peripheral Membrane Proteins
… methods to quantify their interactions inside living cells are very limited. Because peripheral membrane proteins usually exist both in membrane-bound and cytoplasmic forms, the separation of these two populations is a key challenge. This thesis aims at addressing this challenge by extending …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
… including the detection of nucleic acids and living cells. The first project used luminescent lanthanide ion probes that take advantage of hyper-sensitive acquisition of their long-lived emission signal in time- resolved mode, which avoids short-lived background fluorescence of the medium. Two …
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