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Showing 1 to 14 of 14 for “"Liver models"”.
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Proteomic Analysis of Three Dimensional Organotypic Liver Models
In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid …
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The Design of Three-Dimensional Multicellular Liver Models Using Detachable, Nanoscale Polyelectrolyte Multilayers
… of three-dimensional (3D) multi-cellular liver models comprised of primary rat hepatocytes, liver sinusoidal endothelial cells (LSECs), and Kupffer cells (KCs). LSECs and KCs in the liver model were separated from hepatocytes by a nanoscale, detachable, optically transparent chitosan and …
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Developing disease-state liver models to determine the effects on brain function in vitro
The liver is responsible for filtering and detoxifying harmful chemicals from the body. Because of its role as the barrier between the gut and the circulatory system, and due to the brain’s high perfusion rate, the liver’s function as a regulator for blood composition is highly important for the …
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Multi-Cellular Organotypic Liver Models for the Investigation of Chemical Toxicity and Liver Fibrosis
The liver is responsible for lipid and glucose metabolism, protein and bile synthesis and the biotransformation of xenobiotics. These functions, performed by hepatocytes, are dependent on heterotypic interactions with other liver cell types and the stratified microarchitecture of the organ. In …
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From iPSCs to Engineered Heart and Liver Models: Modulating Genetic and Extracellular State for Maturity
The heart and liver are central to human physiology yet remain major sites of drug-induced toxicity, driving the need for robust, scalable, and physiologically relevant in vitro models. Induced pluripotent stem cell (iPSC)–derived hepatocyte-like cells (HLCs) and cardiomyocytes (iPSC-CMs) offer …
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Engineering complex liver models for drug screening and infectious diseases: a biomaterials and co-culture perspective
In vitro liver models have many applications in disease modeling and drug screening. Micropatterned cocultures (MPCCs) of primary human hepatocytes (PHHs) and supportive stromal cells have been shown to display high hepatic functions for long-term drug and disease studies. However, MPCCs lack liver …
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Engineered Liver and Intestine Platforms for Modeling Physiology and Disease
The intestine and liver are connected in vivo via a recirculating blood supply called the gut-liver axis, which plays a critical role in nutrient/drug absorption and metabolism. Given the diversity of roles the liver plays, there is a need for robust in vitro models capable of recapitulating …
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Engineering human hepatic tissue for modeling liver-stage malaria
The Plcsmodium liver stage is an attractive target for the development of antimalarial drugs and vaccines, as it provides an opportunity to interrupt the life cycle of the parasite at a critical early stage. However, targeting the liver stage has been difficult due to a lack of human liver models …
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Fabrication of tissue scaffolds using projection micro-stereolithography
In vitro liver models are a critical tool in pharmaceutical research, yet standard hepatocyte cultures fail to capture the complexity of in vivo tissue behavior. One of the most critical features of the in vivo liver is the extensive microvasculature which allows for the delivery of nutrients and …
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The Design and Assembly of 3D Liver Mimetic Cellular Architectures
We report the assembly of three-dimensional (3D) liver sinusoidal mimics comprised of primary rat hepatocytes, human or rat liver sinusoidal endothelial cells denoted as hLSECs and rLSECs respectively, and an intermediate chitosan-hyaluronic acid (HA) polyelectrolyte multilayer (PEM). The height of …
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The Role of Biomechanics in Liver Tissue Engineering
The field of liver bioengineering aims to generate functioning liver tissue in the laboratory in order to study complicated processes such as development, disease, cancer, drug metabolism, or even to engineer organs that can be implanted in patients. One approach in liver tissue engineering is to …
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Shortwave infrared imaging and its translation to clinically-relevant designs
… of an endogenous disease biomarker in animal models including nonalcoholic fatty liver disease and cirrhotic liver models and models of a neurodegenerative disease pathway. While this biomarker has been known for decades, we describe a method for its noninvasive detection in living animals …
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The Design of Polyelectrolyte Multilayers Using Galactosylated Chitosan
… challenge in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. Hepatocytes have a surface protein, called the asialoglycoprotein …
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Pharmaceuticals in the Environment: Assessing Potential Risks to Fish
… also critically evaluated against other in vitro liver models used to measure pharmaceutical clearance in fish, including subcellular fractions, hepatocyte suspensions and spheroids. This analysis demonstrated the strong utility and broad applicability of the various in vitro models, offering a …