Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Liquid chromatography-tandem mass spectrometry (LC-MS/MS)"”.
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The association between circulating vitamin d levels and body composition in collegiate female athletes
… athletes, between the ages of 18-22, had BMI calculated from anthropometric measurements, body fat percentage assessments via dual energy X-ray absorptiometry (DXA) scans, serum vitamin D levels determined via fingerstick and dried blood spots, followed by liquid chromatography/tandem mass …
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Towards Rapid Measurement of Non-Nucleoside Reverse Transcriptase Inhibitors in a Portable, Low-Cost Platform
… Most DLF approaches measure drug levels via liquid chromatography-tandem mass spectrometry (LC-MS/MS), which can incur high costs and lengthy delays that make it unsuitable for near point-of-care settings. In response, we developed a rapid enzymatic assay for the measurement of non-nucleoside …
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A Data Attribution-Based Approach to Model Diagnosis in LC-MS/MS Structure Prediction
… of small molecules from complex mixtures using liquid chromatography tandem mass spectrometry (LC-MS/MS) is a challenging task with far-reaching implications in many areas such as drug discovery, environmental science and metabolism research. Yet, despite its importance and significant efforts …
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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
A method was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification and confirmation of 8 drugs of abuse (cocaine, codeine, MDMA, methadone, methamphetamine, morphine, nicotine, and oxycodone) and their various metabolites (acetylmorphine, cotinine, EDDP, …
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classCleaner: A Quantitative Method for Validating Peptide Identification in LC-MS/MS Workflows
Because label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics infers the peptide sequence of each measurement, there is inherent uncertainty in the identity of each peptide and its originating protein. Removing misidentified peptides can improve the accuracy and …
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Analisi Molecolare del Melanoma
… by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography¿tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were overexpressed in both melanoma cell lines as compared with melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase …
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Investigating the use of coca and other psychoactive plants in Pre-Columbian mummies from Chile and Peru. An analytical investigation into the feasibility of testing ancient hair for drug compounds.
… Andean populations. This project utilised liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the use of psychoactive plants in individuals from cemetery sites in Chile and Peru by analysing hair specimens for a variety of psychoactive compounds. Hair specimens from 46 …
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Novel Functions of the Transcription Factor Aryl Hydrocarbon Receptor (AHR) and Its Tryptophan-Derived Ligands in Cancer Cells
… and liver cancer cells using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our findings reveal that MYC enhances the intracellular levels of Trp and its metabolites in the kynurenine pathway, specifically increasing the expression of Trp transporters SLC7A5, …
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Determining the Rates of Protein Synthesis in the Zebrafish Heart in Response to Chronic Unpredictable Stress
… of this thesis was to develop a method to calculate the rates of synthesis of heart proteins of zebrafish on a proteome-wide scale. The initial stage of the project involved the optimisation of a method to characterise the protein complement of individual zebrafish hearts. It was concluded …
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RNA-binding proteins mediate anti-inflammatory regulation of vascular disease
… was identified as an HuR interacting protein by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The-HuR-FXR1 interaction is abrogated in RNase-treated extracts, indicating that their association is tethered by mRNAs. FXR1 expression is induced in diseased, but not normal arteries. …
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Investigating the role of routine drug analyses in survivors of sexual offences admitted to the clinical forensic unit at Victoria Hospital
… the utilisation and applicability of a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for common drugs of abuse in cases of suspected drug facilitated sexual offences. Methods: Blood and urine samples from 17 consenting adult survivors, who reported to the Clinical …
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Development of a liquid-liquid, solid-phase extraction (SPE), LC-MS/MS analytical method for qualitative and quantitative drug residue analysis in white-tailed deer
… and managers struggle with animal health problems within the deer industry; as a result, veterinary drugs are used. The administration of antibiotics for treatment of infectious diseases may lead to residues in tissues. The quality and safety of food from animal origin is a growing concern for …
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A prospective study to assess the value of liquid chromatography-tandem mass spectrometry in the management of paediatric poisoning at Red Cross War Memorial Children's Hospital, Cape Town, South Africa
… There is a paucity of data on the role of liquid chromatography-tandem mass spectrometry (LC-MS/MS), in the management of paediatric poisoning in low-and middle-income countries (LMICs). In high-income countries, most studies are retrospective, and few include children. Objective: The study …
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Proteomic Map of ER+ Breast Cancer Cell Cycle
… comprehensive overview of the biological mechanisms, molecular pathways and specific proteins involved in cell cycle progression in ER+ breast cancer cells. We focused on the G1-to-S phase transition of the cell cycle because major differences in cell proliferation mechanisms between normal and …
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Metabolic engineering of the pterin branch of folate synthesis by over-expression of a GTP cyclohydrolase I in peanut
… vitamin quantification will be required. A liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed to identify and quantify the different forms of folate. However, additional work will be needed to determine sensitivity of the instrument, to optimize vitamin …
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Method development and proteomic profiling of boar seminal plasma using mass spectrometry-based workflows
… the use of low fertile boars in breeding systems is prominent. Using mass spectrometry in fertility studies has gained momentum in the race for new markers of fertility and semen cryopreservation. Therefore, the aim of this study was to develop and optimise a mass spectrometry-based proteomic …
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Mass spectrometric studies of ether lipids in Archaea and sediments
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been implemented as a means to separate and detect tetraether lipid cores derived from the complex lipids of Archaea. Distinct dissociation pathways during tandem mass spectrometry were noted for the lipid cores, providing information …
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Evaluation of the Pharmacokinetic-Pharmacodynamic relationship, Metabolism and Plasma Protein Binding of the novel antitumor agent, 2-Methoxyestradiol (2ME2), following oral administration in patients with solid tumors.
… drug concentrations which were determined by liquid chromatography tandem mass-spectrometry [LC/MS/MS, LOQ: 1ng/mL]. Circulating plasma concentrations were very low at all dose levels with high interindividual pharmacokinetic variability. Median plasma half-life was about 1-2 days. The …
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A 'Biorelevant' Approach for Accelerated In Vitro Release and In Vitro-In Vivo Relationship of a Biodegradable, Naltrexone Implant
… for quality assurance of manufactured dosage forms. For drug release testing of sustained release parenteral dosage forms, the modified USP Apparatus 4 (flow-through cell) has been recommended by the The Fédération Internationale Pharmaceutique/American Association of Pharmaceutical Scientists …
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Advances in Amino Acid Analysis for Marine Related Matrices and Its Application to Coastal Shelf Settings in The Canadian Arctic
… acids. Combining ion pairing separation and mass selection software with liquid chromatography tandem mass spectrometry (LC/MS/MS) allows detection of amino acids that were previously difficult to measure due to coelution and incomplete derivatization. The method was validated by examining a …
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