Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"Liquid Biopsy"”.
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OPTIMIZED APPROACHES FOR LIQUID BIOPSY IN GLIOBLASTOMA PATIENTS
… barrier (BBB) limit the accuracy of tissue biopsy and conventional imaging, hampering truly personalised treatment. To overcome these limitations, novel strategies are needed that can non-invasively capture tumour-specific information Objective This thesis explores three complementary, …
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Non-Invasive cancer detection: computational applications in liquid biopsy and radiomics
… of computational approaches for analyzing liquid biopsy and radiomic data. The research presented herein addresses the limitations of current methods in comprehensively analyzing circulating tumor DNA (ctDNA), particularly at low ctDNA fractions, and introduces novel tools and strategies to …
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Advancing Technologies for Precision Medicine: From Liquid Biopsy to Single-Molecule DNA Sequencing
… This dissertation integrates blood-based liquid biopsy assays with an emerging single-molecule nanopore sequencing platform to address limitations associated with conventional tissue biopsies and DNA sequencing technologies in cancer care. First, we demonstrated the potential of …
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The DNA methylation landscape of metastatic prostate cancer: from characterization to liquid biopsy applications
… the design and development of NEMO, a tailored liquid biopsy sequencing panel approach to allow non-invasive neuroendocrine castration-resistant prostate cancer detection in patients with metastatic disease. Based on previous results and the comprehensive analysis of multiple datasets, I …
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Unleashing the potential of liquid biopsy: allele-informed evaluation of plasma samples for cancer patients management
Liquid biopsy and next-generation sequencing of cell-free DNA (cfDNA) in cancer patients’ plasma offer a minimally-invasive solution to detect tumor cell genomic information to aid real-time clinical decision-making. Reliability and sensitivity in the detection of genomic alterations is crucial for …
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Fully Integrated Modular and Mixed-Scale Systems for Processing the Molecular Cargo of Liquid Biopsy Markers
… in nature, like blood or saliva also known as liquid biopsies. Circulating tumor cells, microRNAs, extracellular vesicles (EVs), or proteins are all examples of liquid biopsy biomarkers. Analysis of these biomarkers normally requires a trained operator to run expensive and laborious laboratory …
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A Two-Pronged Approach to Stemming the Opioid Epidemic: Preclinical Strategies for Substance Use Disorder Medications Development and Liquid Biopsy Detection of Inflammatory Pain
… we designed and implemented a proof-of-concept liquid biopsy approach to quantify inflammatory pain with the ultimate goal of enhancing prescribing practices at the point-of-care.
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LIQUID BIOPSY IN GLIOBLASTOMA (GBM)CHARACTERIZATION OF PLASMA EXTRACELLULAR VESICLES CONCENTRATION, SIZE AND CARGO FOR NON-INVASIVE GBM DIAGNOSIS, MONITORING AND IDENTIFICATION OF CIRCULATING BIOMARKERS
… is critical to implement GBM management. Liquid biopsy, particularly through the analysis of Extracellular Vesicles (EVs), offers a promising avenue. We isolated EVs from 2 mL of plasma using Size Exclusion Chromatography and assessed their concentration and size with Tunable Resistive …
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Digital Profiling of Circulating Extracellular Vesicles at Single-Upconversion Nanoparticle Sensitivity and Resolution
… heterogeneity of single EVs, particularly from liquid biopsy sampling, will guide clinical decisions on the stages of tumour progression. However, the nanoscopic sizes (typically 40-200 nm) and the extremely small quantity of cargo materials demand the high detection sensitivity, stability, …
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Detection of Cell-free Tumor DNA in Liquid Biopsies of Dogs with B cell Lymphoma: A Biomarker Discovery
… assessments. We propose a minimally invasive liquid biopsy technique involving blood collection to detect cell-free DNA from tumors using Next-generation sequencing. We hypothesize that identical tumor aberrations can be found in matching plasma and tumor DNA. Five dogs diagnosed with B-cell …
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Nucleosome repositioning in glioblastoma
… and JMJD6, which I propose for the role of new liquid biopsy markers. Moreover, I studied differences in nucleosome occupancy at different classes of genomic features such as ALU repeats, which showed unexpected resemblance to transcription start sites. Finally, I compiled a dataset of genomic …
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Analyzing Extracellular Vesicles for Disease Monitoring in Metastatic Colorectal Cancer Patients
… CRC (mCRCs) and to classify mCRCs, tumor biopsy of a suspected metastasis is often required. The consensus molecular subtype (CMS) classification which is based on gene expression profiles of CRC tumor specimens, is a predictive factor for treatment outcomes of standard chemotherapies for …
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A Method for Identification of Pancreatic Cancer through Methylation Signatures in Cell-Free DNA
… being explored as an approach for non-invasive ‘liquid biopsy’ that can be deployed widely and cost-effectively to screen for early signs of disease. DNA methylation signatures found in cfDNA can serve as a biomarker for detection of cancer. Previous efforts to detect pancreatic cancer using …
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Deep Learning and Optimised Nanoplasmonic Sensors for Label-free Biomedical Applications
… cells. Recently, they have been exploited as liquid biopsy biomarkers for various diseases. In theory, an ultra-sensitive biosensor should be able to detect and identify them from a liquid biopsy sample. Surface Enhanced Raman Spectroscopy (SERS) is an ideal biosensor candidate for the …
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Detection of trace levels of circulating tumour DNA in early stage non-small cell lung cancer
Liquid biopsies, using analytes such as circulating tumour DNA (ctDNA), can detect and quantify cancer in a minimally invasive way and can provide information on tumour heterogeneity. Current limitations in the liquid biopsy field are centred around the general sensitivity of the present assays and …
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Biological, Clinical, and Functional Implications of Cell-Free DNA Topology in Cancer
… clinical studies evaluating cfDNA topology as a liquid biopsy analyte. Next, I examined the impact of topology on the ability of tumour-derived cfDNA to stimulate DNA-sensing pathways in immune cells. Through rigorous mechanistic investigations, I observed pro-inflammatory macrophage signaling in …
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