Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 261 for “"Lipopolysaccharide (LPS)"”.
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Safety of Slow Release Lipopolysaccharide (LPS) Pellet in a Swine Model and Development of an Exogenous Reference Control for Real Time PCR
… and immune status, and genetics). Microbial lipopolysaccharide (LPS) translocation from the gastrointestinal tract into systemic circulation may play a role in the development of diseases characterized by CIAO. Therefore, markers or characteristics of an altered immune response induced by LPS …
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Gangliosides와 oxidized low density lipoprotein (oxLDL) 에 의한 신경교세포의 활성조절 및 그 기전에 대한 연구
… signaling pathways 7 1. JAK-STAT pathway 7 2. Lipopolysaccharide (LPS) –induced signaling pathways 8 3. Liver X receptor (LXR) pathway as anti-inflammatory signaling Pathway 9 D. Aims of study 10 II. MATERIALS AND METHODS 11 A. Reagents 11 B. Cell culture 12 C. Electrophoresis mobility shift …
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The Effects of Neonatal Immune System Activation with Lipopolysaccharide on Adolescent and Adult Anxiety Behaviours in Male and Female Rats
… 3 and 5) acute immune system activation with lipopolysaccharide (LPS) on adolescent and adult anxiety behaviours. The major findings suggest that neonatal LPS does not have general long-lasting effects on adolescent anxiety and locomotor behaviours. Rather, early endotoxin treatment has highly …
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Toll-Interacting Protein Regulation of Low-grade Non-resolving Inflammation
… molecular pattern molecules (PAMPs) such as lipopolysaccharide (LPS). To differentiate signal strengths, innate leukocytes may utilize distinct intra-cellular signaling circuitries modulated by adaptor molecules. Toll-interacting protein (Tollip) is one of the critical adaptor molecules in …
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Novel Characteristics of Murine Bone Marrow-Derived Macrophages and Human Macrophage-Like Cells
… increase in response to the bacterial endotoxin lipopolysaccharide (LPS). Generation of these macrophage-like cells was not repeatable with other samples of human bone marrow, but the cells continue to proliferate in cell culture and will be investigated further in future studies.
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Modulation of the Serotonin Reuptake Transporter in RAW264.7 Macrophages
… of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and …
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Proposed in vitro model of neutrophil swarming in a chronic, low-level inflammatory state
… modeled with low dose stimulants such as lipopolysaccharide (LPS). While the innate immunity plays a significant role in fighting infectious disease, an initial exposure to low dose LPS hinders secondary infection clearance and pre-disposes murine models for fatal sepsis. Neutrophils are …
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The immune-modulating activity of Sutherlandia frutescens
… frutescens were stimulated in vitro with either Lipopolysaccharide (LPS) or Phytohaemagglutinin (PHA). Results show that Sutherlandia frutescens is not toxic at any of the concentrations tested. The addition of Sutherlandia frutescens at high concentrations to the stimulated whole blood cell …
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Fumarate Inhibits TNF-α Release
… bacterial endotoxins, including Gram-negative lipopolysaccharide (LPS) and Gram-positive lipoteichoic acid (LTA). And respond by releasing tumor necrosis factor-α (TNF-α). While beneficial for neutralizing acute pathogen exposure, prolonged TNF-α release results in chronic inflammation and …
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Expression and Function of Toll-Like Receptor 2 and 4 in Human Melanocytes
… blot, flow cytometry and immunocytochemistry. Lipopolysaccharide (LPS) was used as microbial stimulator on melanocytes. The expression of TLR2, TLR4 and MyD88 at mRNA and protein level were increased after LPS stimulation. LPS affected cell proliferation and melanin synthesis. TLR2 expression …
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DNA Sequence and Haplotype Variation Analysis of Inflammatory Response Genes NLRX1, IL6, and IL8 in the Turkey (Meleagris gallopavo)
… response in the turkey using a microbe-based lipopolysaccharide (LPS), an approach previously described for the chicken. A total of 18 SNPs was identified in the three genes screened in this project: Interleukin 6 (IL6) and 8 (IL8), and NLRX1. Mortality data from the LPS challenge were not …
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The Production of Nitric Oxide By Murine Peritoneal and Alveolar Macrophages in Response to Candida Albicans Yeast and Hyphae
… yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did …
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Early life immune and physical stress directly influences anxiety-like behaviour in adolescent rats: examining sex differences
… acute immune activation with the endotoxin, lipopolysaccharide (LPS) on postnatal days 3 and 5 on adolescent anxiety-like behaviour in rats before and after a stress period. Previous research has shown that adults rats exposed to LPS during the neonatal stage show anxiety-like behaviour …
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Utilization of TMV-TEMPO as an in Vivo MRI Sensor of ROS Production in Liver Inflammation and as a DNP Agent
… as an in vivo T2 -weighted MRI sensor of ROS in lipopolysaccharide (LPS)-induced liver inflammation in Balb/c mice. Chapter 4 details the 13 C DNP testing of TMV-TEMPO as a polarizing agent and the use of gadolinium coordination polymer GduDEP as a 13 C DNP signal enhancer. Finally, the overall …
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HIV-1 Transgenic Rats Display Alterations in Immunophenotype and Cellular Responses Associated with Aging
… Age matched animals were challenged using lipopolysaccharide (LPS) to determine immunological modification of HIV-1Tg animals throughout the aging process. LPS is known to cause imbalances in cytokine and chemokine release, and provides a method to identify alterations of immune response to …
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Temporal Transcriptional Regulation of IL-10-Induced Anti-Inflammatory Genes in LPS-Triggered Macrophages
… through Toll-like receptor 4 (TLR4) binding to lipopolysaccharide (LPS). IL-10 acts on these cells through the induced expression of genes that inhibit expression of select TLR-induced pro-inflammatory genes. Our understanding of the mechanisms by which cells respond to IL-10 with the …
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Quantitative analysis of TLR-4-mediated cell responses in murine macrophages
… pathogen-associated molecular patterns such as lipopolysaccharide (LPS), TLR-4 can in tandem initiate a pair of downstream signaling pathways to regulate cytokine/chemokine release, endotoxin tolerance and apoptosis, which have been suggested to directly or indirectly participate in the …
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The Involvement of Interleukin-1 Receptor-Associated Kinase-1 (IRAK-1) as a Critical Modulator of Macrophage Migration
… phorbol 12-myristate 13-acetate (PMA), or lipopolysaccharide (LPS) was significantly decreased in IRAK-1-/- murine macrophages compared with wild type cells. Mechanistically, we first demonstrated that IRAK-1 works downstream of PKCε and directly binds to VASP, a cytoskeleton regulatory …
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TGFβ signalling in microglia and the impact of microglia-specific Smad4 deletion
… TGFβ signalling in microglia and the effect of lipopolysaccharide (LPS)-induced reactive state on microglia TGFβ signalling. Advanced gene expression profiling techniques, such as Nanostring nCounter technology, were utilized to examine microglia-specific gene expression during development and …
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The Effect of O Antigen Loss on the Protein Composition and Inflammatory Response Elicited by Klebsiella pneumoniae
… of the outer membrane components such as lipopolysaccharide (LPS) and outer membrane proteins and contain cytosolic and periplasmic proteins in the lumen. K. pneumoniae is often found to lack an O antigen. The absence of the O antigen has been reported to alter the protein content of the …
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