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Showing 1 to 20 of 53 for “"Lipoic acid"”.
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Studies on lipoic acid metabolism
The covalent attachment of lipoic acid to the lipoyl domains (LDs) of the central metabolism enzymes pyruvate dehydrogenase (PDH) and oxoglutarate dehydrogenase (OGDH) is essential for their activation and thus for respiratory growth in the bacterium Escherichia coli and the yeast Saccharomyces …
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Lipoic acid metabolism in Leishmania major
… to the available carbon and nitrogen sources. Lipoic acid (LA) is a multifaceted molecule, and plays an important role as a water- and fat-soluble antioxidant. LA is also an essential cofactor of the alpha-ketoacid dehydrogenase complexes (alpha-KADHs) and of the glycine cleavage complex (GCC). …
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Studies of Lipoic Acid Related Enzymes
… shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the physiological application of this enzyme in E. coli fatty acid metabolism, I constructed the first E. coli fabH …
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Lipoic acid protein ligases in Plasmodium spp.
… that parasite-specific inhibition is feasible. Lipoic acid metabolism in Plasmodium differs from that of mammals in some ways and therefore it might be a promising target for the development of new antimalarials. This study investigated the importance of lipoic acid ligation in P. falciparum …
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Studies on the biosynthesis of lipoic acid
… for the detection and quantitation of Iipoic acid has been developed. Lipoic acid produces a yellow color when reacted with PdCl₄⁻² in 1 N HCI. The colored complex formed is extractable into methylene chloride, which can be readily concentrated, increasing the color intensity. The limit of …
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Studies of Lipoic Acid Metabolism in Escherichia Coli
Lipoic acid is a disulfide cofactor which is essential for function of several key enzymes. The studies presented in this thesis shed new light into the understanding of how lipoic acid is synthesized and incorporated into proteins in Escherichia coli. In Chapter Two, the nature of the real …
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Enzymatic diversity in lipoic acid modification of proteins
… characterized and complete metabolic model for lipoic acid metabolism. In this thesis I make discoveries through analysis of the diversity of lipoic acid metabolic genes in other organisms. By examining the distribution and phylogeny of the lipoate ligase protein family, I and others have found …
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Post-Translational Modification With Lipoic Acid in Escherichia Coli
Lipoic acid is a cofactor essential for the function of key metabolic pathways in eukaryotes and most prokaryotes. Physiological function of lipoic acid is dependent on covalent attachment of the cofactor to highly specific lysine residues of enzymes complex subunits. E. coli encodes two distinct …
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Lipoic acid assembly on 2-oxoacid dehydrogenases in E.coli
Lipoic acid is a covalently attached cofactor essential for the activity of 2-oxoacid dehydrogenases and the glycine cleavage system. In the absence of lipoic acid modification the dehydrogenases are inactive and aerobic metabolism is blocked. In Escherichia coli two pathways for the attachment of …
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α-Lipoic Acid Attenuates Ischemia Reperfusion Injury of the Rat Liver
… The present work investigated the potential of α-lipoic acid (LA), a well established agent in the therapy of diabetic polyneuropathy, to reduce IRI of the rat liver. In the system of the isolated perfused rat liver, an experimental model of hepatic IRI, LA was utilized in different treatment …
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Studies of lipoic acid and biotin metabolism in bacteria and higher eukaryotes
My PhD research focus has been the study of lipoic acid and biotin metabolism in several bacteria. Lipoic acid and biotin are essential enzyme cofactors found in all three domains of life. Both molecules function only when covalently attached to key metabolic enzymes, 2-oxoacid dehygrogenase (OADH) …
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The protective effect of α-lipoic acid in doxorubicin induced cardiotoxicity in rats.
This study examined the protective effects of α-lipoic acid in rats exposed to a cardiotoxic dose of the widely used chemotherapy agent doxorubicin. α-Lipoic acid significantly enhanced the survival of rats exposed to a lethal dose of doxorubicin (p<0.001). Morbidity was proportionally higher in …
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The effects of R(+)-lipoic acid supplementation on regulation of human skeletal muscle pyruvate dehydrogenase
… 28 d of supplementation with 1000 mg R(+)-lipoic acid in young sedentary males (age, 22.1 ± 0.67 yr, body mass, 78.7 ± 10.3 kg, n=9). In certain individuals, lipoic acid decreased the 180-min area under the glucose concentration and insulin concentration curve during an oral glucose …
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Characterisation of Anti-Apoptotic Signalling Pathways in Hepatocytes activated by alpha-Lipoic Acid and Atrial Natriuretic Peptide
Both, the R-enantiomer of the antioxidant alpha-lipoic acid (R-LA) and the hormone atrial natriuretic peptide (ANP) are known to exert potent hepatoprotective action. The present work characterises alpha-lipoic acid- and ANP-mediated signal transduction pathways involved in the regulation of …
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Selenotrisulfide Derivative Of Alpha-lipoic Acid: Evaluation In A Cell Culture Model For Potential Use As A Topical Antioxidant
… to covalent modifications of lipids, nucleic acids, and proteins within a cell. This damage has been implicated in the development of cancer and degenerative diseases. As the skin is the first level of defense for UV radiation, skin cancer is an obvious concern. Previous studies have …
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Investigations into the Metabolic Requirements for Lipoic Acid and Lipid Species During the Life Cycle of the Malarial Parasite Plasmodium Berghei
… biology of Plasmodium parasites and their hosts. Lipoic acid is a short eight-carbon chain that serves a number of different functions with the cell. By disrupting a key enzyme in the lipoic acid synthesis pathway in the rodent species of malaria, Plasmodium berghei, we sought to investigate its …
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Covalent post-translational modification of proteins in Escherichia coli: Genetic and biochemical studies of biotin and lipoic acid biosynthesis and function
… distinct classes of mutants involved in either lipoic acid biosynthesis or the covalent attachment of lipoic acid to proteins in E. coli. Two of these classes of mutants were isolated as Tn1000dKn insertion mutants and both classes map to min 14.5 on the E. coli chromosome. The other two classes …
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The Effects of Various Therapeutics on Cystine Stone Formation
… lab had shown that the nutritional-supplement α lipoic acid was an effective inhibitor of cystine stone formation. Here, we continued to further confirm the effects of α lipoic acid as an inhibitor and preventer of stone growth. Due to the unknown mechanism of α lipoic acid, we hypothesized α …
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A comparison of sulfhydryl active compounds on activation and germination of Bacillus thuringensis spores
… sulfhydryl active compounds used were oxidized lipoic acid and cystine (S-S), cysteine HCl and dithiothreitol (S-H). B^. thuringiensis spores incubated in cysteine HCl had a substantially lower germination rate compared to spores incubated only in Tris pH 7.5 buffer. \^en B^. thuringiensis …
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