Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"Lipidomic"”.
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Evaluating Lipidomic Perturbations in Biological Systems Using Cheminformatic Data Analysis Capabilities.
North Carolina State University Theses Chemistry.
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Lipidomic Dysregulation in Alzheimer's Disease: Relation to Genetics, Neuroimaging and Other Biomarkers
Large-scale genome-wide association studies for Alzheimer’s disease (AD) have identified more than 20 risk loci and several pathways including lipid metabolism. Lipids are fundamental to cellular structure and organization, where they compose biological bilayer membranes surrounding the cell. In …
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Differential Lipidomic and Proteomic Responses Induced by Sub-lethal Drug Challenge in Susceptible and Drug Resistant Mycobacterium smegmatis
Tuberculosis remains a major global health challenge and the increasing strength and prevalence of drug resistance threaten to undo much of the good progress made. As one of the primary, frontline anti-tuberculous drugs, growing resistance to rifampicin in particular is concerning. Sub-lethal drug …
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A metallomic, proteomic and lipidomic investigation of the malaria parasites digestive vacuole and insights into the mediators of haemozoin formation
… of detergent. Mass spectrometry-based lipidomics identified and quantified over 400 lipids in dissolved haemozoin. Neutral lipids were found to be the dominant class comprising 90% cholesterol, 2% cholesterol esters and 0.6% acylglycerols. The detected lipids were used to prepare a …
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Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting
Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The …
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The Role of Cardiac Imaging in Deep Phenotyping Patients with Cardiomyopathy
… The third project explored metabolomic and lipidomic changes in 85 subjects (TTNtv, obesity and healthy controls). Notably, TTNtv carriers without phenotypic DCM, already exhibited distinct maladaptive metabolic alterations, findings further amplified in established DCM. Obesity also …
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THE PROPHYLACTIC POTENTIAL OF DOCOSAHEXAENOIC ACID, AN OMEGA-3 FATTY ACID, IN TRAUMATIC BRAIN INJURY
… The following studies involve investigating the lipidomic alterations that occur as a result of supplementation of ω3 FAs in vitro and in vivo. Docosahexaenoic acid (DHA) supplementation had the most effective incorporation into neural cells in vitro when compared to other ω3 FAs. Next, DHA was …
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Defining the genetic and molecular pathways underlying motor neuron degenerative diseases
… cell model, showed that loss of CEPT1 altered lipidomic profiles alongside disrupted endoplasmic reticulum (ER) and mitochondrial morphology and function. Chapter four outlines recent findings of two novel candidate disease genes, PLPP1 and C6orf120, as a cause of neurological disorders with …
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Functional Study of Lipoxygenase-mediated Resistance to Fungal Pathogens in Maize for Milk-chain Safety and in Grapevine for Sustainable Viticulture
… of VviLOX isoforms and JA and SA-related genes, lipidomic and phytohormonal profiles between the genotypes. Stable Agrobacterium-mediated gene knockout (through CRISPR/Cas9) of VviLOXO and 7, and overexpression of VviLOX13 were performed on embryogenic calli of V. vinifera cv. Sugraone-a genotype …
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Investigating the Relation Between Genetic Variation in FADS1 & FADS2 with the LCPUFA Content of Extracellular Vesicles from Human Milk
… of lactation. High sensitivity quantitative lipidomic analysis of fatty acids in human milk was completed by gas chromatography-mass spectrometry. Saliva samples were analyzed with pyrosequencing for SNPs rs174546 (FADS1) and rs174575 (FADS2) genotyping. The fatty acid content of EVs from …
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Lipoprotein lipidomics in obesity-associated metabolic and hepatic disorders
… HDL and VLDL fractions where we performed lipidomic analyses. As opposed to VLDL, the HDL lipidome was characterised by PL and PUFA depletion. These changes have been reported in hepatic lipidomic studies of NAFLD patients, thus suggesting a close link between peripheral tissues and the …
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The Interaction of Obesity and Age and their effect on Adipose Tissue Metabolism in the Mouse
… Here, I have conducted a comprehensive lipidomic and transcriptomic study of white adipose tissue in mice that become obese either through genetic modification (ob/ob genotype), diet (high-fat diet) or a combination of the two across the life course. Specifically, I demonstrated that the …
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Stage-Specific Multiomic Analysis of Human Erythroid Differentiation
… potential. Untargeted LC-MS/MS–based lipidomic analyses were performed to determine changes in EE lipid metabolite profiles dependent on Epo. Finally, intracellular flow cytometry was used to validate expression of the enzymes involved in lipid metabolism. Results: Based on the …
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Pathophysiological changes in cardiomyocyte metabolism and bioenergetics during stress-induced cardiomyopathy.
… chromatography (HPLC) and mass spectrometry for lipidomic spectrum detection, real-time polymerase chain reaction (RT-PCR) and Western blot for relative expression of lipid transporters were used to study cardiomyocyte metabolism. Confocal microscopy was used to study Ca2+ handling. The presented …
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Evaluating Phosphatidylinositide as Lipid Biomarkers for Hyperglycemia-related Triple-negative Breast Cancer Aggressiveness
… matrix (ECM), and cell accumulation, with lipidomic analyses of cell membranes under normal and high-glucose conditions. Our findings demonstrate that hyperglycemia enhances aggressive traits in TNBC cells, promoting increased movement and growth, but no effect on ECM attachment. In …
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The design and numerical analysis of a microfluidicssystem for osteogenic differentiation of mesenchymal stemcells.
… a characteristic profile of the metabolomicand lipidomic factors that could allude to key biomarkers which could be used to assessthe proposed system.
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INDUCED PLURIPOTENT STEM CELL DERIVED LIVER MODEL FOR THE STUDY OF PNPLA3-ASSOCIATED NON-ALCOHOLIC FATTY LIVER DISEASE
… by various functional, genomic, and lipidomic assays. The genetically edited sublines showed similar differentiation efficiency toward hepatocytes as untargeted cells indicating that changes in PNPLA3 activity does not affect hepatic development. Following treatment with free fatty …
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Omics approach towards understanding the function of bioactive lipids in osteosarcoma
… aimed at discovering the various proteomic and lipidomic biomarkers in metastatic and non-metastatic osteosarcoma as well as development of lipid mediators as potential therapeutics. We take a high-throughput approach towards identifying specific membrane proteins and lipids that are …
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APPLICATION OF DATA ANALYTICS AND SPECTROSCOPY ON THE CHEESEMAKING PRODUCTION CHAIN
… tandem mass spectrometry. For the first time, a lipidomic study is conducted to differentiate vegetable-renneted (Cardoon) cheese samples from calf rennet-based product. The interaction between different rennet sources and cheese ripening times is also considered. Due to the high dimensionality …
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Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells
… differentiation and polarisation, and lipidomic analysis identified<br/>significant regulation of arachidonic acid and glycerophospholipid metabolism.<br/>Furthermore, a novel stable isotope labelling methodology was developed using<br/>deuterium oxide (2H2O) in conjunction with high …
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