Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Lipidation"”.
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
… (PE), in a process known as lipidation. Lipidation of Atg8 family proteins regulates numerous facets of the autophagy process, including regulating expansion of the phagophore membrane, recruiting selected cargoes for degradation, and providing an autophagosome membrane-bound …
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Lipid acquisition by apolipoprotein A-I in ER and Golgi compartments of primary mouse hepatocytes
… the significance and location of intracellular lipidation of newly synthesized apoA-I in hepatocytes were in plasma HDL formation. By labeling primary mouse hepatocytes with 3H-choline, we showed that phospholipidation of apoA-I is most significant in endoplasmic reticulum (ER) and medial Golgi …
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The role of CASM in the endolysosomal damage response
… activated, and subsequently mediated the lipidation of ATG8 (e.g. LC3A) family proteins directly onto damaged lysosome membranes. Specifically, this damage-induced lipidation occurs via the central V-ATPase – ATG16L1 axis, and onto phosphatidylserine (PS), a highly specific hallmark of …
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Chemical Reporters for Investigating Lipidated Proteins at the Host-Pathogen Interface
<p>Lipidation of proteins regulates many cellular processes such as signaling transduction and membrane sorting by modulating protein localization and proteinprotein interactions. As such, defects in protein lipidation can render host cells more susceptible to microbial infection and are also …
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Self-assembly and aggregation of glucagon-like peptide 1 and its analogues
… of two sets of chemical modification strategies, lipidation and C-terminal amidation, on the physical stability of the peptide. The first part of this work combines experimental and computational approaches to better understand the molecular basis of the aggregation of GLP-1 and its C-terminally …
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Novel mechanisms of Atg16L1 recruitment in non-canonical autophagy
… this process is the recruitment, processing and lipidation of LC3 to autophagosomes. Recently it has become increasingly apparent that, through the unconventional use of some autophagy related proteins, LC3 can also become lipidated to distinct non-autophagosomal membranes of the endolysosomal …
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The APOE Pathway as a Modulator of Amyloid Pathology in Alzheimer's Disease Models
… regulates the expression of ABCA1 and apoE lipidation, although its effect on amyloid pathology is unknown. Additionally, apoE variants can modulate AD risk. The apoEε4R251G variant eliminates the increased risk associated with the APOEε4 allele. This variant is located within the lipid …
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Pharmacological characterisation of selective Y4 and dual Y2/Y4 receptor agonists: novel targets for putative anti-obesity therapies
… subsequently modified by either PEGylation or lipidation to improve their relatively short half-life and pharmacokinetic profile. This thesis presents a detailed study of the in vitro pharmacological data performed to aid the progression of the most promising peptide candidates. An assessment …
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The Development and Application of Ultra-sensitive Protein Aggregate Detection Techniques
… disease (AD). The isoform-specific and lipidation-specific behaviours of apoE and the co-aggregates formed with A β are explored. The discussion proposes potential therapeutic strategies involving the promotion of apoE lipidation. Then, the focus shifts to the development of …
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Studies of the Physical Stability of GLP-1 & Chemically Modified Forms of GLP-1
… such as short lifetimes and low stability. Lipidation of peptides is widely established as a means of increasing stability in vivo. However, relatively little is known about the effect of lipidation on peptide self-assembly and other aggregation phenomena in vitro. The aim of this study is …
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Role of ATG 5 in ER stress-induced apoptosis in meniscus.
… 5 protein levels was associated with decreased lipidation of LC3 and increased activation of Cleaved Caspase 3, which promoted meniscus cell apoptosis. Pretreatment of meniscus cells with 4-phenyl butyric acid (PBA), a small molecule chemical chaperone that alleviates ER stress or with MG-132, a …
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REDEFINING THE SCOPE OF PRENYLATION: DISCOVERY OF “FORBIDDEN” SUBSTRATE RECOGNITION AND DEVELOPMENT OF METHODS UTILIZING PRENYLATED PROTEINS
… quantitative method introduced here is Protein-Lipidation Quantitation (PLQ); a new method that can simultaneously measure the amounts of a non-lipidated substrate protein and its lipidated product in a cellular context. In PLQ, use of a fluorescent protein fused to the substrate under …
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The Role of Lipid-Free Apolipoprotein A-I and PCPE2 in HDL Metabolism
… the opening mechanism for lipid-free apoA-I lipidation to form recombinant HDL (rHDL) and nascent HDL (nHDL). Results of these studies identify central helices 4-6 as essential for rHDL and nHDL formation.
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UNDERSTANDING THE RAL GTPASES: THE REGULATION BY PROTEIN LYSINE FATTY ACYLATION, SIRT2, AND INTERACTING PROTEINS
… regulated by lysine fatty acylation. Lipidation, such as cysteine palmitoylation and prenylation, is a key regulatory mechanism for small GTPases. Recently it has been reported that lysine fatty acylation also regulates several small GTPases. My graduate work shows that RalB, but not …
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Search For The Functions That Mediate Protein Palmitoylation And Depalmitoylation
… This protein has several potential lipidation sites and a thioesterase domain, characteristics it shares with known thioesterases.</p>
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Regulation of mTORC1 by homocysteine and its effects on autophagy in human and mouse neuronal tissues
… of ULK1 which consequently reduces LC3 lipidation. mTORC1 also dampens TFEB-driven transcription of autophagy-related genes resulting in a buildup of abnormal proteins, including [beta]-amyloid and phospho-Tau. Formation of these protein aggregates leads to AD-like neurodegeneration. …
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Engineering delivery systems for the treatment of Non-Hodgkin lymphoma
… poor cell penetration and serum instability. Lipidation of peptides, producing peptide amphiphiles, has been shown to allow for hydrophobically-driven self-assembly into peptide amphiphile micelles. There are many advantages to employing peptide amphiphile micelles as drug delivery devices, …
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ATG8 Regulation and Function in Non-Canonical Autophagy
… to this membrane recruitment is an unusual lipidation process. In canonical autophagy, it is widely accepted that the lipid species conjugated to ATG8 proteins is phosphatidylethanolamine (PE). However, using mass spectrometry, this study shows for the first time in vivo that ATG8 proteins …
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Modulating Amyloid Beta Clearance by Altering Cellular and Whole Brain Apolipoprotein E Metabolism
… A1 (ABCA1), a protein that regulates apoE lipidation, have previously been shown to modulate brain amyloid beta levels. In the work presented in this dissertation, we found that increasing LDLR levels enhanced the cellular uptake and degradation of amyloid beta by primary astrocytes, and …
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Mechanisms of Genome Buffering and Cell Fate Coordination in Adult Tissue Homeostasis
… and the maintenance of adult tissues. Lipidation of Wnt proteins by the acyltransferase Porcupine (Porcn) is crucial for secretory pathway exiting. Using chemically based approaches, I have demonstrated that Porcn active site features conserved across animals enforce ω-7 cis fatty …
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