Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Lipid II"”.
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Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials.
<p>Natural products belonging to the lipid II-binding family act as potent antimicrobial agents by disrupting cell wall biosynthesis via sequestering the late-stage intermediate lipid II. However, the emergence of resistance mechanisms and poor bioavailability have hindered the utility of these …
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Understanding the Genome-Wide Response of Streptomyces coelicolor to the Glycopeptide Antibiotic Teicoplanin
… on the cell wall peptidoglycan intermediate lipid II, interfering with peptidoglycan biosynthesis and weakening the cell wall. A general resistance mechanism to these antibiotics requires a core set of genes, vanRSHAX, that detect a glycopeptide (VanS) and upregulate genes (VanR) which …
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Biosynthesis and engineering of lanthipeptide natural products for novel applications
… the class I lanthipeptide cyclase NisC and class II lanthipeptide synthetase HalM2 that, indeed, the conjugate addition reactions are reversible and that the enzymes can open up all thioether rings in their products. The studies described in Chapter 5 focus on the mode of action of haloduracin …
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The biosynthesis and discovery of lanthipeptides
… Chapter 4, I investigated the mode of action of lipid II-targeting lanthipeptides. The binding event between nisin–lipid II and Halα–lipid II were characterized using isothermal titration calorimetry. Collectively, these studies further expanded our knowledge on lanthipeptides biosynthesis and …
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Organization of the Streptococcal ExPortal and its Interaction with Cationic Antimicrobial Peptides
… the secretory translocons at a defined anionic lipid microdomain of the cytoplasmic membrane that is enriched for accessory factors needed for protein biogenesis, referred to as the ExPortal. The manner in which <italic>S. pyogenes<italic/> achieves and maintains spatial coordination of the …
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Regulation of Surface Proteins Assembly on the Wall of Gram-Positive Bacteria
… the protein to the peptidoglycan precursor lipid II. This thesis deals with the spatial regulation of the sorting reaction, and the role sortase localization plays in the correct distribution of surface proteins. We have introduced a new immunofluorescence procedure to study the distribution …
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Biosynthesis and mode of action of ribosomally synthesized and post-translationally modified antimicrobial peptides
… as the cell wall peptidoglycan precursor lipid II. Through the in vitro biosynthesis of haloduracin, several analogues of Halα were prepared and evaluated for their ability to inhibit peptidoglycan biosynthesis as well as bacterial cell growth. In an effort to overcome the limitations of …
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Development of lanthipeptide engineering strategies
… variant that binds the small molecule target lipid II. Finally, the ability of the nisin-displaying phage to function as a broad host-range virion with potential applications in the genetic transformation of difficult-to-transform bacteria or in a therapeutic setting was demonstrated. …
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Raman spectroscopy for the microbiological characterization and identification of medically relevant bacteria
… with peptidoglycan precursor proteins and lipid II as observed in the Raman spectra of these cells. Raman spectra were also acquired from the isolated cell envelope fraction of the cells expressing different forms of wag31 and the results showed that a significant number of the molecular …
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Intramembrane signal transduction and cell envelope stress response in <i>Bacillus subtilis</i>
… welches auf Zellwandantibiotika, die den Lipid II-Zyklus hemmen, und auf Zerstörung der Cytoplasmamembran reagiert. Es wird von den letzten beiden Genen des liaIHGFSR Lokus kodiert. Ohne Zellhüllstress werden die letzte! n vier Gene des Operons von einem schwachen konstitutiven Promotors …
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Exploration of the biosynthesis of lanthipeptides
… a dehydratase and a cyclase, while class II lanthipeptides adopt a bifunctional enzyme. Trifunctional enzymes with independent evolution pathways are employed by class III and class IV lanthipeptides. A major subclass of lanthipeptides possess antimicrobial activity, known as lantibiotics …
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Inquiries into the Mycobacterial Caseinolytic Protease System
… strong positive interactor, gatD, involved in lipid II and peptidoglycan biosynthesis, was found to be a putative novel ClpC1 substrate through recognition of a C-terminal degron. A plate suppressor screen found that suppression of the succinate dehydrogenase, sdh, or succinyl-CoA synthase, …