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Showing 1 to 20 of 58 for “"Lipid Binding"”.
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Lipid Binding in Worked Wheat Flour Doughs
A study has been made of factors affecting lipid binding in wheat flour doughs. Simple flour water doughs, wetted by a method which avoided the introduction of mechanical work, were used to follow changes in lipid binding during the initial stages of dough mixing. Varying the moisture content of …
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Bioinformatics Discovery And Functional Characterization Of Lipid-Binding Lov Photoreceptors
… light. LOV photoreceptors are modular flavin-binding proteins that consist of discrete sensor and effector domains. Blue-light drives a LOV sensor to trigger a conformational change in photoreceptor structure that ultimately regulates the biochemical function of one or more of its fused …
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Regulation of rho guanine nucleotide exchange factors through lipid binding and phosphorylation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
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Solution Structure and Characterization of Lipid Binding of the NOXO1β PX Domain
The NADPH oxidases are multiprotein enzyme complexes which catalyze the formation of reactive oxygen species (ROS). The most studied of these is the phagocytic NADPH oxidase (PHOX), which is found in neutrophils, macrophages and monocytes. The PHOX enzyme plays a role in the host immune response …
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Isolation and Lipid Binding Properties of the Low Molecular Weight Protein Components of Bovine High Density Lipoprotein
… and stoichiometry under a variety of initial lipid: protein ratios. Recombinants with D(,4) were less discrete, with the size and stoichiometry of complexes being dependent on the initial lipid: protein ratio used. The complexes formed are of a size (2 to 3 x 10('5) daltons) and weight …
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Ssp1p is a lipid binding protein involved in shaping of the prospore membrane during meiosis in S. cerevisiae
… this structure. I can show here that Ssp1p is a lipid binding protein and this affinity is probably required for anchoring the protein complex in the membrane. Ssp1p would then act like a hinge, connecting the PSM to Ady3p, another protein of the LEP coat. Deletion of SSP1 results in a block of …
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Circular dichroism studies of Escherichia coli pyruvate oxidase and secondary structure and lipid binding properties of the alpha peptide from pyruvate oxidase
… undergoes a change in conformation with the binding of TPP and pyruvate. Circular dichroism measurements show the change in secondary structure with this ligand induced conformational change. Circular dichroism spectra also change with the addition of phospholipid vesicles which bring about …
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Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
Lipids and proteins are two major components of cells, which are crucial for structural integrity and maintenance of cellular homeostasis by integrating various extracellular and intracellular cues. Lipids are involved in the regulation of cellular functions and can interact with proteins and …
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The Tumor Suppressor BRCA1 in IP3R Calcium Signaling and Apoptosis
… by protein-protein interactions, including binding to proto-oncogenes and tumor suppressors to regulate cell death. Here we show that the IP3R binds to the tumor suppressor BRCA1. BRCA1 binds directly to the IP3R and causes destabilization of the IP3R closed state resulting in an increased …
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Lipid-protein interactions of immunoreceptor signaling subunit cytoplasmic domains
Protein-lipid interactions are emerging as key components of cellular processes such as protein and membrane trafficking and cell-cell signaling. Many proteins bind lipid reversibly, including cytoplasmic proteins involved in signal transduction, such as Ras and Src. Membrane binding is vital for …
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Nanoscale Organization of The Small Gtpase Rac1
<p>Rac1 is a small, guanine-nucleotide binding protein that cycles between an inactive GDP-bound and active GTP-bound state to regulate actin-mediated motility, migration, and adhesion. Plasma membrane (PM) localization is essential for its biological activity. Rac1 PM targeting is directed by a …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… motility and metabolism via their primary lipid product phosphatidylinositol 3,4,5- trisphosphate (PtdIns(3,4,5)P3) and a metabolite of this, phosphatidylinositol 3,4- bisphosphate (PtdIns(3,4)P2). Many effector proteins for PtdIns(3,4,5)P3 are well recognised but by contrast, few molecular …
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Understanding Membrane Curvature Sensing
… compositions and shapes. Consequently, membrane-binding proteins are tuned to recognise and modify these membrane states to perform their functions. To study the curvature sensitivity of proteins, I have developed a single-particle assay using NanoSight technology that tracks the Brownian motion …
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The APOE Pathway as a Modulator of Amyloid Pathology in Alzheimer's Disease Models
… sporadic AD, with apoE protein crucial for brain lipid transport. ATP-binding cassette subfamily A member 1 (ABCA1), another risk gene, loads lipids onto apoE, highlighting the importance of lipid homeostasis in AD. MicroRNA-33 regulates the expression of ABCA1 and apoE lipidation, although its …
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Structural Characterization and <em>in vitro</em> Antiproliferative Activity of Non-Specific Lipid Transfer Protein 1 (nsLTP1) from Fennel (<em>Foeniculum vulgare</em>) Seeds
<p>Non-specific lipid transfer proteins (nsLTPs) are cationic proteins involved in intracellular lipid shuttling, in growth and reproduction, as well as in defense against pathogenic microbes. Even though the primary and spatial structures of some nsLTPs from different plants indicate their similar …
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Molecular Architecture of the ER-Mitochondria Encounter Structure
… are consistent with the known length of the lipid binding domains of the two ERMES components for which a structure is known. Separately, quantitative live-cell fluorescence microscopy was used to determine the total number and stoichiometry of ERMES components within contact sites. With …
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EFFECTS OF RESTRICTIVE DISULFIDE BONDS IN THE VINCULIN TAIL DOMAIN
… thought to undergo conformational changes upon binding actin or acidic phospholipid. For the present study, cysteine residues were inserted to form disulfide bonds to prevent motion in the arm (residues 1048-1066), strap (residues 878-896) or both regions. The ability of these mutant domains to …
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High-throughput functionalization of the Toxoplasma kinome uncovers a novel regulator of invasion and egress
… homology to mammalian PDK1, SPARK lacks a lipid-binding domain, suggesting a rewiring of the pathway in parasites. HiT screening extends genome-wide approaches into complex cellular phenotypes, providing a scalable and versatile platform to dissect parasite biology.
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… C-terminal membrane anchor exhibits exquisite lipid-binding specificity for select species of phosphatidylserine (PtdSer). We therefore investigated whether reducing PM PtdSer content is sufficient to abrogate KRAS oncogenesis. Oxysterol-related binding proteins ORP5 and ORP8 exchange PtdSer …
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Investigations into membrane protrusions mediated by IRSp53 IMD
… domains, which can induce membrane curvature by binding to membranes. It is still unsure whether IMD activity is dependent on actin or membrane interactions, or both. Data are presented here showing that actin destabilizing drug cytochalasin D prevents extension of IMD protrusions, suggesting a …
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