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Showing 1 to 20 of 49 for “"Lipases"”.
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Studies on soil and microbial lipases
… lipase and two partially-purified bacterial lipases. Lipase activity was dependent upon substrate surface area rather than substrate concentration per se. Long-chain insoluble fatty acids were inhibitory to lipase activity. A minimum concentration of fatty acid was required before inhibition …
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Comparative characterisation of esterases and lipases for biosensors
Twenty four carboxylesterases and lipases from various microbial sources were compared with commercial pig liver esterase in order to determine which would be most appropriate for use in a biosensor application and in the elucidation of the relationship between protein hydration and gas phase …
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Engineering lipases and solvents for trans/-esterification of used vegetable oils
<p>Diminishing petroleum reserves and increasing environmental awareness has led to an urgent need to develop alternative fuels, such as biodiesel. However, the conventional method to produce biodiesel uses environmentally harmful chemical catalysts. A relatively new development in the production …
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Substratselektivität von Lipasen bei der Veresterung und Umesterung cis-trans-isomerer Fettsäuren und Fettsäureester
… determination of substrate selectivity of lipases towards unsaturated fatty acids and fatty acid esters with respect to position and configuration of C=C-double bonds. First, the substrate selectivity of 39 lipases from microorganisms, plants and animal tissue has been examined in the …
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Charakterisierung und rationale Immobilisierung von Lipasen in biphasischen Reaktionssystemen
Despite the broad use of lipases in ester synthesis procedures, crucial aspects on optimal pH and enzyme behaviour in biphasic reaction media are still not well-known. Such information is highly desired for the rational process design. Therefore, the influence of the pH in lipase-based …
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Proteome-wide Functional Profiling of Serine Hydrolases in the Human Malaria Parasite
… the parasite is essential for parasite survival. Lipases catalyze the hydrolysis of ester bonds of lipid species such as neutral lipids and phospholipids. Although roles of lipases in propagation, as well as virulence in various organisms, have been acknowledged, in P. falciparum lipases remain …
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Probing the Structure and Function of Lecithin Cholesterol Acyltransferase by Site-Directed and Deletion Mutagenesis
… between LCAT and other interfacially activated lipases and have contributed to the understanding of the functional regions of LCAT.
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Study of porous magnetic nanocomposites for bio-catalysis and drug delivery
… adsorption and chemical bonding. The immobilised lipases were used in hydrolysis of pNPP and hydrolysis of cis-3,5-diacetoxy-1-cyclopentene to investigate the catalytic activity of the immobilized enzymes compared to free enzymes. The results indicated that free lipases provided slightly higher …
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Imobilização de lipase em criogel supermacroporoso para a síntese de lipídios estruturados
… esse propósito é interessante a aplicação de lipases (E.C.3.1.1.3) na sua forma imobilizada devido a vantagens como a reutilização do biocatalisador, a facilidade de separação do produto final e o potencial para aplicações em processos contínuos. Recentemente, os criogéis supermacroporosos têm …
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Aplicação de microrganismos lipolíticos em alimentos e na biodegradação de poliuretanos
… quanto para aplicação biotecnológica. As lipases são enzimas responsáveis principalmente, pela hidrólise dos triacilgliceróis e amplamente exploradas comercialmente. Além da hidrólise, conseguem catalisar a reação reversa, a esterificação e, também reações como transesterificação, …
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Dessimetrização e enriquecimento enantiomérico de derivados do triciclo[5.2.1.0 2,6] decano através de enzimas e de indutores quirais. Preparação dos respectivos pseudopeptídeos restritos
… altos rendimentos, usando catálise enzimática (lipases) em reações de transesterificação. A resolução do respectivo diéster racêmico através da hidrólise catalisada com esterase (PLE) não forneceu o monoéster opticamente enriquecido; enquanto que a dessimetrização do anidrido usando indutores …
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Functional Characterization of Serine Hydrolases Mediating Lipid Metabolism and Protein Depalmitoylation in Asexual Stage Plasmodium Falciparum
… encodes more than 50 SHs including proteases, lipases, esterase and others, while only several of them have been characterized. The study of uncharacterized SHs will shed light on future drug development to treat malaria. In this study, we applied chemical biology and genetic approaches to …
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INSIGHT ON ANGPTL3 IMPACT ON HEPATIC METABOLISM AND FUNCTION
… of VLDLs. Its deficiency leads to higher lipases activation, resulting into hypolipidemia and protection from cardiometabolic risk. While the role of circulating ANGPTL3 on lipases has been well established, its effect on hepatic homeostasis and metabolism is still unclear. This work aims …
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Mikrobinių lipolizinių fermentų imobilizavimas ir imobilizuotų preparatų analizė /
… being paid to the microbial enzyme class of lipases (E.C. 3.1.1.3), whose functional versatility and unique conformational structure allow the hydrolysis and synthesis of triacylglycerols. These properties are advantageous in a wide range of industrial applications, but the use of free-form …
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Immobilisation of Bio-molecules on Magnetisable Solid Supports for Applications in Bio-catalysts and Bio-sensors
… first application involved bio-catalysis where lipases [Pseudomonas Fluorescens lipase (PFL) and Candida Rugosa lipase (CRL)] were chemically conjugated (covalently linked) via glutaraldehyde-modification onto the amino-functionalised nanoparticles for applications such as: (i) hydrolysis of …
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Various factors that affect hydrolytic rancidity in rice bran
… is prone to hydrolytic rancidity due to active lipases. To assess the loss of quality, free fatty acids (FFA) in raw bran were measured over 48 hour period. Cultivar, farming technique, harvest year, milling conditions and degree of milling (DOM) were the factors examined for their effects on …
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Minimization of extracellular enzyme production by Pseudomonas fluorescens in a model milk system by controlled oxygen atmospheres
… would minimize the synthesis of proteinases and lipases by P. fluorescens in a model milk system was determined in our study. Two litre volumes of Ultra-High Temperature sterilized milk (2 % m.f.) in 4 litre carboys were equilibrated with a continuous supply of atmospheric oxygen between 1 - 21 …
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Directed evolution of a subtilisin Carlsberg variant towards improved perhydrolytic activity for industrial application
… protease and likely other hydrolases (e.g. lipases or esterases) having perhydrolytic activity or find new enzymes with peroxycarboxylic acid production by screening of metagenome libraries. Subtilisin Carlsberg was furthermore the first protease engineered through directed evolution towards …
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Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
Extracellular proteases and lipases from strains P26, 32A, Pl, and P27 of Pseudomonas fluorescens were produced using a dialysis membrane method. The proteases were partially purified by molecular exclusion chromatography (MWC0=60,000) and selected biochemical characteristics were determined. …
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