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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Lipase Activity"”.
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Studies on soil and microbial lipases
… of four methods for the determination of lipase activity was found to be dependent upon the source of the enzyme, when comparisons were made using commercial porcine pancreatic lipase and two partially-purified bacterial lipases. Lipase activity was dependent upon substrate surface area …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… of multiple bgs mutants. Additionally, secreted lipase activity levels were evaluated in bgs13 strains expressing either the wild type or the mutated MAT&agr; signal peptide. The results indicated that regardless of their differences, the MATα secretion signals and bgs mutants transported their …
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Minimization of extracellular enzyme production by Pseudomonas fluorescens in a model milk system by controlled oxygen atmospheres
… would minimize the synthesis of proteinases and lipases by P. fluorescens in a model milk system was determined in our study. Two litre volumes of Ultra-High Temperature sterilized milk (2 % m.f.) in 4 litre carboys were equilibrated with a continuous supply of atmospheric oxygen between 1 - 21 …
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Study of porous magnetic nanocomposites for bio-catalysis and drug delivery
… also used as enzymes (Pseudomonas Fluorescens Lipase (PFL) and Candida Rugosa Lipase (CRL)) supports in catalysis reactions. The enzymes were immobilised onto nanoparticles through physical adsorption and chemical bonding. The immobilised lipases were used in hydrolysis of pNPP and hydrolysis …
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Fat metabolism in the exercising thoroughbred horse
… in a comparable increase in plasma total lipase activity. When co-administered with a triglyceride emulsion, pentosan polysulphate resulted in a similar increase in plasma FFA concentration relative to that produced with the same triglyceride emulsion and heparin. The anticoagulative …
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Lipoprotein Lipase Inhibitor Removal During Ultrafiltration of Renal Failure Patients
… The effect of the plasma on the lipolytic activity of bovine milk lipoprotein lipase was investigated. The lipoprotein lipase activity, as measured by release of C<sup>14</sup>-labeled free fatty acid from triglyceride, was decreased 8-67% when incubated with uremic plasma as compared to …
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Comparison of two ultrafiltration membrane systems for whole milk feta cheese production : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Massey University, Auckland, New Zealand
… degree value method was used to determine the lipase activity in feta cheese. It was found from the composition, texture and sensory analysis that the cheese from the hollow fibre pilot plant was different from the cheese manufactured at Puhoi Valley Cheeses (PVC). The spiral wound cheeses were …
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Proposed Mechanism Explaining Seasonal Biological Foaming in Activated Sludge Systems; Foam-Causing Bacteria Specialize in Consuming Lipids
… tested by studying the relationship between lipase (lipid hydrolyzing enzyme) activity, temperature, and foam occurrences. Lipase activity was found to be higher in foaming plants and very sensitive to temperature, substantiating the role of temperature in seasonal biological foaming. The …
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In vitro examination of the effect of orlistat on the ability of the faecal microbiota to utilize dietary lipids
… and/or orlistat affect the composition and/or activity of the faecal microbiota. Results from 24-h batch culture fermentation experiments demonstrated no effect of orlistat in the presence or absence of a dietary lipid (olive oil) on the composition of bacterial communities [as determined by …
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In vitro examination of the effect of orlistat on the ability of the faecal microbiota to utilize dietary lipids
… and/or orlistat affect the composition and/or activity of the faecal microbiota. Results from 24-h batch culture fermentation experiments demonstrated no effect of orlistat in the presence or absence of a dietary lipid (olive oil) on the composition of bacterial communities [as determined by …
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Lipid accumulation and utilization during microcycle growth of Aspergillus niger.
… of triacyl glycerol was indicative of changes in activity of lipolytic enzymes. However little lipase activity was detected, although enzymes which hydrolysed water-soluble esters were more readily assayed. In order to assess the relative utilization of each of the carbon substrates glucose, …
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The effects of yarrow (<i>Achillea millefolium</i>) and herbal supplements on growth performance and nutrient utilisation of broiler chickens
… effects of dietary yarrow on digestive enzyme activity and nutrient availability of birds fed control and low nutrient density basal diets. Yarrow supplementation increased intestinal lipase activity and improved diet AME in birds fed high nutrient density diets but not in birds fed low …
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Non-invasive assessment of gastrointestinal function using breath test technology : investigations in health and disease.
… gas produced; and (3) improvement in pancreatic lipase activity with pancreatic enzyme replacement is inversely related to the gastric emptying time. Breath testing was also used to show that substrates with a natural ¹³C-enrichment can be used to assess small intestinal enzyme activity. Breath …
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Proteome-wide Functional Profiling of Serine Hydrolases in the Human Malaria Parasite
… targets. In this study, we have employed activity-based protein profiling (ABPP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to functionally characterize SHs. In our effort to profile plasmodial SHs using ABPP, we have identified a human erythrocyte SH, acylpeptide …
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Biophysical Insights into Lipid Oxidation Effects on Membrane Self-Organization and Enzymatic Catalysis Under Extreme Conditions
… an immobilized form of Candida antarctica Lipase B (CALB), as a catalyst in the transesterification reaction of para-nitrophenyl palmitate (pNPP) and glycerol under extreme temperature conditions. Electrospray Ionization Mass Spectrometry (ESI-MS) and spectrophotometric analyses …
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Nicarbazin for contraception of Canada geese: laboratory and field studies
… affects reproduction are unknown. Lipoprotein lipase, vitellogenin, transglutaminase, and calcium are all involved in egg formation and embryogenesis. Therefore, in vitro assays were used to evaluate four potential mechanisms of action of NCZ on egg formation and embryogenesis. First, an assay …
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Comparative characterisation of esterases and lipases for biosensors
Twenty four carboxylesterases and lipases from various microbial sources were compared with commercial pig liver esterase in order to determine which would be most appropriate for use in a biosensor application and in the elucidation of the relationship between protein hydration and gas phase …
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The regulation of postprandial lipemia in man
… (some of which are known to increase lipoprotein lipase activity in vitro) did not increase the rate of chylomicron-triglyceride clearance. If the quantity of fat in a meal was fixed, then postprandial lipemia increased with increasing saturation of the triglyceride fatty acids. These differences …
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