Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Lineage commitment"”.
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pRb's role in cell fate, lineage commitment, and tumorigenesis
… re-activation was sufficient to switch the fate commitment of osteosarcoma tumor cells in vitro through direct regulation of transcription factors that control mesenchymal differentiation. Consistently, we found that reactivation of Rb in tumors generated from Rb;p53 DKO cells was sufficient to …
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Congenital Heart Defect-Associated Enhancers Shape Human Cardiomyocyte Lineage Commitment
Pages vii-xiv are misnumbered as pages vi-xiii.
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Functional analysis of the histone variant H2A.Z during lineage commitment
… this thesis, we dissect the role of H2A.Z during lineage commitment. In particular, we focused on the Polycomb-mediated mono-ubiquitylation of H2A.Z. We found that this modification regulates the differentiation potential of mouse embryonic stem cells (mESCs). Loss of H2A.Z ubiquitylation leads to …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
Polycomb group proteins are key transcriptional repressive complexes involved in cell fate decisions during development and cell identity maintenance during homeostasis (1). Polycomb group proteins primarily assemble into two different complexes: Polycomb repressive complex 2 (PRC2) with the …
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Analyses of hematopoiesis and lineage commitment from ES and iPS cells
Die Kultivierung embryonaler Stammzellen (ES Zellen) und ihre Differenzierung zu verschiedenen Arten hämatopoietischer Zellen in vitro wurde optimiert und standardisiert, um eine zeitlich definierte, quantitativ auswertbare zelluläre Entwicklung zu verfolgen.Diese standardisierten in …
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Roles of Polycomb Repressive Complex 2 in differentiation and lineage commitment
Contains fulltext : 310092.pdf (Publisher’s version ) (Open Access)
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CROSSTALK BETWEEN PRC1 AND PRC2 IN INTESTINAL LINEAGE COMMITMENT AND CELL IDENTITY
… control transcriptional silencing and secretory lineage commitment. Unexpectedly, we have found that removal of PRC1.1 rescued the effects of PRC2 inactivation in lineage skewing while maintaining extensive transcriptional derepression, revealing an intimate connection between distinct Polycomb …
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Roles of growth hormone in liver growth and mesenchymal stem cell myogenic and adipogenic lineage commitment
… growth; 2) to determine the effects of GH on the commitment of mesenchymal stem cells (MSCs) to myogenic and adipogenic lineages. In the first study, the GH-deficient lit/lit male mice were injected (s.c.) daily with rbGH or vehicle for two weeks. GH-injected lit/lit mice tended to have a greater …
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Role of Notch Signaling and GATA-3 in Establishing T-lineage Commitment and Survival in Developing Thymocytes
… is required to commit progenitors to the T-lineage and acts in concert with many transcription factors such as GATA-3 to fully induce this fate choice while inhibiting the B-cell lineage. Although both Notch and GATA-3 are indispensible for thymocyte development, their precise roles in …
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Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment
… they commit the T cells to distinct functional lineages. Insight into how thymocytes are nonetheless able to distinguish between these two signals is crucial to our fundamental understanding of T-cell development and how it might be manipulated therapeutically. Pertinently, the pre-TCR is …
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Control of chromatin accessibility during CD4+ T cell development and activation
… differentiating into highly specialised cellular lineages that either promote or suppress immune reactions. Whereas effector T (Teff) cells drive immune activation and promote clearance of infections and cancer, regulatory T (Treg) cells suppress their function to prevent excessive immune …
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Mechanisms of CD4+ Regulatory T Cell Heterogeneity in Health and Disease
… factor BACH2 is critical for early Treg cell lineage specification, however, its function following Treg lineage-commitment is unresolved. The studies detailed herein demonstrate that Bach2 is highly expressed during Treg cell development in the thymus. High levels of Bach2 are maintained in …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… Although PRC2 has critical functions in lineage commitment and in mediating cell fate transitions, it has proved difficult to study its precise role in these processes since complete loss of H3K27me3 leads to an inability of embryonic stem cells (ESCs) to properly undergo directed …
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Multiscale Modeling Of Cell Fate Switching To Predict Patient-Specific Response To Combination Cancer Therapy
… of regulatory mechanisms controlling cellular lineage commitment and encoded in the unique epigenome of each cell type. Recent experimental studies with induced pluripotent stem cells have allowed researchers to investigate the dynamic nature of cell identity and relationships between gene …
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The Role of AF9 and AF9-Mediated Protein Interactions in Hematopoiesis and Leukemogenesis
… to the erythroid, myeloid and megakaryocytic lineages in the presence or absence of Af9. Based on previously published studies, we hypothesized that loss of Af9 would result in a loss of erythroid colony formation. Our results indicate no significant difference in erythroid, myeloid or …
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Toll Like Receptor Dependent Control of T Helper 17 Cell Differentiation and Inflammasome Activation
… of CD4+ T cells. While IL-12 is critical for Th1 lineage development, IL-6 and TGF-b play an important role in differentiation of Th17 cells in vitro. IL-1 has also been implicated in regulating Th17 differentiation. In this dissertation, we examined the relative contribution of IL-6 and IL-1 for …
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Stochastic gene expression during lineage specification of single T helper lymphocytes
… the differentiation of which requires numerous lineage specifications at various developmental stages. The precise control of immune cell differentiation and the delicate balance of their population composition are crucial for effective protection against infectious environmental agents, without …
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Molecular mediators of cardiac-specific enhancer activation
… regulator of enhancer activation during cardiac-lineage commitment. We then used an inducible degron-tag strategy to conditionally deplete TEAD1 and observed an abnormal beating phenotype in CMs. Further mechanistic studies revealed that TEAD1 was necessary for the activity of a subset of cardiac …
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Isolation and characterization of CEABF-1, the ABF-1 homolog in C. elegans
… as cellular proliferation and differentiation, lineage commitment, sex determination, neurogenesis, myogenesis, hematopoeisis and pancreatic development. The free-living nematode Caenorhabdits elegans is an important model organism. Genetics studies of a gene in nematodes help us to better …
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Towards using organoids in functional genomics – A single cell characterisation of mouse gastruloids
… the epigenetic processes underlying this lineage commitment remain poorly understood in mammals. Large-scale perturbation studies are necessary to functionally study the epigenetics of mouse gastrulation. While it has thus far not been feasible to perform such studies at scale in vivo, …
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