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Showing 1 to 20 of 32 for “"Limited Proteolysis"”.
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Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
Limited proteolysis was used to study the domain structure and to produce a large N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I and dipalmitoyl phosphatidylcholine (DPPC) or palmitoyloleoyl PC (POPC) by …
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Protein Characterization by Mass Spectrometry
… of proteins to be probed. The method uses limited proteolysis to distinguish regions within a target protein that are solvent exposed and flexible from those that are solvent inaccessible or conformationally rigid. The DNA binding protein Max was investigated by the procedure and several …
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The role of N-linked glycosylation on the structure and function of somatic angiotensin-converting enzyme
… Unfortunately, the protein was susceptible to limited proteolysis in the interdomain linker region, suggesting that key glycans might shield the linker region from proteolysis. Furthermore, a loss in expression of MG-sACE was observed. These observations prompted the investigation of the effect …
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Development and Application of Large-Scale Protein Folding Stability Analysis in Drug Target Identification and Disease Biomarker Discovery
… bottom-up proteomics readout in proteome-wide limited proteolysis experiments. Another goal of this work is to apply these methods to the target identification of two drugs with known mode of action, and to the biomarker discovery of Parkinson’s disease.</p><p>Described in the first part of the …
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Hard and soft coatings on gold nanoparticles at the nano-bio interface
… gold nanoparticles by an adapted BCA assay and limited proteolysis.
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Active Center Structure and Sequence Studies on Bacterial Luciferase Utilizing the Essential Cysteine, Protease-Labile Region, and Delta Fragment
… essential cysteine. These results and those of limited proteolysis of luciferase modified at this cysteine with ('3)H-N-ethylmaleimide, N-terminal sequence analysis of the resulting fragments, and specific cleavage of (alpha) at the essential thiol caused by modification with …
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
… human LARP6, the SUMO tag was cleaved by Ulp1. Limited proteolysis studies were used to compare the domain topology of the fish LARP6 proteins to the human homolog. The RNA binding activity of all LARP6 proteins for the COL1A1 stem loop RNA from the appropriate species was measured by …
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The tissue factor-factor VII(a) complex in blood coagulation
… zymogen FVII can be activated to FVIIa via limited proteolysis at a single peptide bond by many proteases, including factor Xa, factor IXa, thrombin, and factor XIIa, as well as FVIIa in complex with TF. By using a FVII mutant (S344A), in which the active site serine residue has been mutated …
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Biophysical characterization of KO513, a protein expressed in human invasive glioblastoma
… K0513. Using tryptophan fluorescence assay and limited proteolysis, nucleotides were found to have no significant effect on the tertiary structural conformation of K0513. Pull down assay shows promising interactors of K0513 from fibrosarcoma cell line; future studies will identify the …
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Interaction of HspBP1 with Hsp70
… has only two structural domains as determined by limited proteolysis. Domain I, amino acids 1-83, is largely unstructured. Domain II, amino acids 84-359 is predicted to be 43% helical using circular dichroism. It was also shown that domain II is sufficient for binding to the Hsp70 ATPase domain …
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1, Structural and Functional Studies of Human Replication Protein A; 2 DNA Damage Responses and DNA Repair Defects in Laminopathy-Based Premature Aging.
… foot-printing, fluorescence spectroscopy and limited proteolysis. The data show that upon hyperphosphorylation RPA undergoes a subtle structural change involving its DNA-binding domain B (DBD-B), reducing its affinity for short ssDNA. These results suggest that hyperphosphorylation may …
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Role of the RING domain in MDM2-mediated ubiquitination of p53
… of MDM2 towards p53. Furthermore I show by limited proteolysis and hydrogen deuterium exchange that UbcH5α can be allosterically activated by MDM2. A novel peptide phage display technique linked to next generation sequencing was developed to further confirm an allosteric change and …
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Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus
… For REC domain, itasser homology model and limited proteolysis revealed a two-domain structure connected by a flexible linker. Based on B-factor of linker truncation may be necessary for crystallization of the domain. In this study we initially sought to gain structural information on VarS, …
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Localization of a quinol oxidase domain of the cytochrome d complex of Escherichia coli
… of the model are confirmed in this work via proteolysis and antibody binding experiments. Previous studies characterized two monoclonal antibodies which bind to subunit I and specifically block the ability of the enzyme to oxidize ubiquinol. In this work, the epitopes of both these monoclonal …
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Investigating intermediates in 6-methylsalicylic acid biosynthesis
… were unsuccessful. Limited proteolysis of both holo and apo 6-MSAS found that the apo synthase rapidly lost a C-terminal fragment while holo 6-MSAS was much more stable under the same conditions. Attempts were made to express the acyl carrier protein domain from …
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Interaction of alpha-synuclein with nanoscale systems that mimic synaptic vesicles
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Effects of co-expression of chaperone combinations on production of soluble plasmodial protein PfAdoMetDC in E. coli
… of purified PfAdoMetDC protein was assessed by limited proteolysis and also by conducting activity assays to evaluate the activity of the protein. The PfAdoMetDC protein expressed with supplementation of PfHsp70+DnaJ and KPf+DnaJ combinations was not completely degraded by proteinase K and its …
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Characterisation of XvPrx2 : a type II peroxiredoxin isolated from the resurrection plant Xerophyta viscosa (Baker)
… in a conformational change as determined by limited proteolysis. An in vitro DNA protection assay showed that, in the presence of either XvPrx2 or XvV76C, DNA protection occurred. In addition, an in vivo assay showed that increased protection was conferred on cell lines over-expressing either …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
… of the C-terminus.</p> <p> Sequence analysis and limited proteolysis indicated that a 60-70 residue loop partitions the sulfate transporter and anti-sigma factor (STAS) domain in the C-terminus. To investigate the structural and functional role of the ‘loop’ (residues 571-635), the sequence was …
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Purification and characterization of a smooth muscle myosin phosphatase from turkey gizzards
… is highly specific for myosin and MLC20. The limited proteolysis of SMP-III results in a 1.5- and 2.3-fold increase in the Vmax for MLC20 and HMM (heavy meromyosin), respectively. The increase in activity correlates with the loss of a peptide of approximately 1 kDa from the C terminus. …
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