Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Light-sheet"”.
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Time Resolved Light Sheet Microscopy
… This thesis also demonstrates two new methods of light sheet microscopy, that use both new equipment made available because of technological developments to allow time resolved imaging and traditional microscopic aspects to form a light sheet system based on polarisation. It outlines the design …
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Light-sheet microscopy used for tracking particles
… has generally been limited to 2D culture dishes. Light-sheet microscopy, a recent advance which was awarded Nature Method of the Year in 2014, allows fast, non-invasive 3D imaging across an entire organism. This works by decoupling illumination and detection such that the microscope only …
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Developmental tissue dynamics using non-diffracting light-sheet microscopy
… do not affect the development of the sample. Light-sheet based microscopy has become the method of choice, and has allowed new studies of development on a systems level. Nonetheless, imaging of higher organisms remains challenging due to scattering and a limited depth of focus of the …
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Validation of a Confocal Light Sheet Microscope using Push Broom Translation for Biomedical Applications
… deep chamber of the R-MHD device, an epitaxial light sheet confocal microscope (e-LSCM) was used to improve axial resolution. The linear sensor, having small pixels, blocked out-of-plane light while eliminating the need for a mechanical aperture which is used for traditional point-scanning …
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… describes the further development and use of light-sheet based fluorescence microscopy (LSFM) designed and optimised for imaging the early stages of development of chick embryos. The hardware and software of this microscope were optimised for improvements in performance in data acquisition …
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Light Sheet Microscopy and Image Analysis of Neural Development and Programmed Cell Death in C. Elegans Embryos
… In the past decade, implementations of highly light efficient methods such as single plane illumination microscopy have rendered it possible to follow development of embryonic structures in 3D with excellent temporal resolution (Huisken et al., 2004) and low phototoxicity. Recent work has …
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Low-Cost and High-Throughput Optofluidic Add-on Device for Light Sheet Imaging of Larval and Adult C. Elegans
… we have made modifications to our low-cost light sheet platform, allowing us to capture high-content cross-sectional images of nematodes' nervous systems at earlier stages of development and with higher throughputs. Our platform was put to the test in imaging larval and adult pan neural …
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Measurement of two-dimensional concentration fields of a glycol-based tracer aerosol using laser light sheet illumination and microcomputer video image acquisition and processing
… dimensional concentration fields using a laser light sheet to illuminate the plane of interest, which is captured and processed using current microcomputer-based video image acquisition and analysis technology. Point concentrations, determined optically using extinction of monochromatic …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
… localization precision of fluorescent emitters. Light sheet fluorescence microscopy is a simple solution in which a thin plane of light is used to optically section the sample, resulting in an increased signal-to-background ratio and thus improving the achievable localization precision of single …
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Label-free Optical Approaches for the Non-invasive Assessment of Embryo Quality
… This approach can capture information on how light changes as it passes through cells, or it can capture the natural fluorescence emitted by endogenous molecules, termed autofluorescence. Thus, label-free optical imaging holds great promise for the evaluation of preimplantation embryo quality. …
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Development of novel imaging technology to study cell signalling
… microscope cantilever to create a 2 μm thick light sheet. Using this setup, we demonstrated that ldSPIM can deliver single fluorophore labelled proteins onto the plasma membrane of HEK293 cells or into the cytoplasm and characterise the interaction between cells and the delivered molecules in …
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Imagining the Voltage of Neurons Distributed Across Entire Brains of Larval Zebrafish
… thesis presents a high-speed remote scanning light-sheet microscope capable of imaging GEVI-expressing neurons distributed throughout entire brains of larval zebrafish at a volumetric rate of 200.8 Hz. The microscope combines remote refocusing and an ultrafast dual-camera system to …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… The first section details the design of a light sheet microscope capable of high-throughput imaging of cleared, macroscopic samples with cellular resolution. In light sheet microscopy, the combination of spatially confined illumination with widefield detection enables multi-megapixel …
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Super-resolved volumetric optical imaging of virus-infected cells
… them hard to visualise using diffraction-limited light microscopes. Yet, ‘seeing viruses’ is essential to unravel their infection mechanism within the organisms they highjack, which is key to understanding how such small entities can spark disruptive pandemics. This thesis shows how expansion …
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Generalist 3D Cell Phenotyping for All-Type Tissues
Tissue-clearing methods, light-sheet microscopy, and antibody labeling enable extracting cellular and subcellular information, producing large amount of image data needs to be analyzed. Hundreds of heterogeneous cell types were detected through the data obtained across species and types of tissues. …
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Development of optical systems and imaging analyses for hyperspectral microscopy and 3D super-resolution imaging
… the end, we demonstrate a new configuration of a light sheet microscope that is capable of reconstructing a high-contrast 3D whole-cell image with the use of a single objective lens. The optical layout of this light sheet microscope, the optical alignment procedures, the instrumentation, and the …
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Augmenting Wiring Diagrams of Neural Circuits with Activity in Larval Drosophila
… pan-neuronal calcium indicators is imaged in a light sheet microscope, which are then structurally imaged with high throughput electron microscopy. Methods and tools are provided for the assembly of these image volumes, spatial registration between imaging modalities, automated detection of …
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The Molecular Regulation of Wiring Specificity in the Serotonin System Across Key Postnatal Stages
… during development. Recent research has highlighted the diversity within midbrain serotonin neurons, revealing heterogeneity in connectivity, electrophysiology, and gene expression. Prior studies have identified distinct cortical and subcortical subsystems with varying transcriptomic …
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