Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Light sheet microscopy"”.
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Time Resolved Light Sheet Microscopy
… photon counting fluorescence lifetime imaging microscopy is just one of the tools used to carry out this line of research. Here we demonstrate a direct interaction between two proteins involved in gene regulation and expression; p21 and FMN2. Furthermore, we also show the capability of this …
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Light-sheet microscopy used for tracking particles
Fluorescence microscopy is one of the cornerstones of modern biology but has generally been limited to 2D culture dishes. Light-sheet microscopy, a recent advance which was awarded Nature Method of the Year in 2014, allows fast, non-invasive 3D imaging across an entire organism. This works by …
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Developmental tissue dynamics using non-diffracting light-sheet microscopy
… do not affect the development of the sample. Light-sheet based microscopy has become the method of choice, and has allowed new studies of development on a systems level. Nonetheless, imaging of higher organisms remains challenging due to scattering and a limited depth of focus of the …
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… describes the further development and use of light-sheet based fluorescence microscopy (LSFM) designed and optimised for imaging the early stages of development of chick embryos. The hardware and software of this microscope were optimised for improvements in performance in data acquisition …
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Light Sheet Microscopy and Image Analysis of Neural Development and Programmed Cell Death in C. Elegans Embryos
… creating these structures. New developments in microscopy have made this an excellent time to study neural development in the C. elegans embryo. In the past decade, implementations of highly light efficient methods such as single plane illumination microscopy have rendered it possible to follow …
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Label-free Optical Approaches for the Non-invasive Assessment of Embryo Quality
… This approach can capture information on how light changes as it passes through cells, or it can capture the natural fluorescence emitted by endogenous molecules, termed autofluorescence. Thus, label-free optical imaging holds great promise for the evaluation of preimplantation embryo quality. …
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Generalist 3D Cell Phenotyping for All-Type Tissues
Tissue-clearing methods, light-sheet microscopy, and antibody labeling enable extracting cellular and subcellular information, producing large amount of image data needs to be analyzed. Hundreds of heterogeneous cell types were detected through the data obtained across species and types of tissues. …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… in modern biological research. Fluorescence microscopy has emerged as an indispensable tool in generating such maps, but common techniques are limited by fundamental physical constraints which render them incapable of simultaneously achieving high spatial and temporal resolution. This thesis …
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Imagining the Voltage of Neurons Distributed Across Entire Brains of Larval Zebrafish
… and signal-to-noise ratio requirements of GEVIs, microscopy hardware to date has only been able to image the voltage of neurons within subregions of the brain, even for small animals like the larval zebrafish. To address this challenge, this thesis presents a high-speed remote scanning light-sheet …
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Optimization Techniques for Multi-object Detection and Tracking on Live-cell Fluorescence Microscopy Images and Their Applications
Fluorescence microscopy is a pivotal imaging technique to visualize biological processes and has been extensively utilized in live-cell morphology analysis. Despite its utility, related object detection and tracking tasks still face challenges due to large data scales, inferior data quality, and …
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Super-resolved volumetric optical imaging of virus-infected cells
… them hard to visualise using diffraction-limited light microscopes. Yet, ‘seeing viruses’ is essential to unravel their infection mechanism within the organisms they highjack, which is key to understanding how such small entities can spark disruptive pandemics. This thesis shows how expansion …
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Augmenting Wiring Diagrams of Neural Circuits with Activity in Larval Drosophila
… pan-neuronal calcium indicators is imaged in a light sheet microscope, which are then structurally imaged with high throughput electron microscopy. Methods and tools are provided for the assembly of these image volumes, spatial registration between imaging modalities, automated detection of …
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Organization principles of the early Drosophila embryo
… as cortical migration. In this study, using light sheet microscopy and quantitative analysis, we track individual nuclei in 3D over the migrating stage and demonstrate that cortical migration is essentially a nuclear packing problem resulting from uniformly expanding nuclear shell or the …
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Integrative and Scalable Pipeline for Multi-omic Characterization of Biological Tissues
… we developed a pipeline that involves light-sheet microscopy and nuclei-based co-registration. We achieved multi-omic and multiplexed characterization of thick mouse brain tissues using our pipeline that enabled cell type analysis and ontogeny studies. Furthermore, effective …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Automatic Cell Tracking For Studying Large Epithelial Sheets Of Cells And Its Application To Chicken Gastrulation
… active motions.To answer these questions we use light sheet microscopy to acquire large volumetric time-lapse image sequences covering more than half of the chick embryo. We developed an algorithm to automatically extract the apical cell surface from the acquired volumetric image sequences. …
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Neutrophil biomechanical properties and immune function in health and inflammatory disease
… properties were analysed by RT-DC, lattice light-sheet microscopy, confocal microscopy and scanning electron microscopy. Neutrophil immunologic functions (e.g. NETosis, macropinocytosis) were imaged using florescence microscopy. To analyse the contribution of biomechanical properties to …
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Advancing Fluorescence Microscopy Techniques for Volumetric Whole-cell Imaging
… whole-cell imaging based on fluorescence microscopy. The first project establishes a robust single-molecule localisation microscopy (SMLM) pipeline for volumetric imaging in the whole cell using the double-helix point spread function (DH-PSF) approach. The practical challenges associated …
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Examining Mechanisms Regulating Microtubule Assembly and Function
… total internal reflection fluorescence (TIRF) microscopy to examine the dynamics of individual microtubules assembled from these distinct heterodimers. I found that microtubules assembled with βIIB are substantially more stable, switching from a state of growth to a state of shrinkage (termed …
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