Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Light Field Microscopy"”.
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Single Molecule Localisation with Fourier Light Field Microscopy
… resolution of 3D Single Molecule Localisation Microscopy (SMLM). This technique combines the super-resolution capability of SMLM with the principles of parallax; multiple perspective views of photo-switchable fluorophores are captured using a lenslet array in the Fourier plane of a fluorescence …
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Light field microscopy for 3D imaging flow cytometry
… unable to answer certain biological questions. Light field microscopy is a computational imaging technique that captures 3D information in a single camera shot. In contrast to many other methods of 3D imaging, light field requires only a simple optical setup and no moving parts—making it ideally …
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Vortex Light Field Microscopy for 3D Spectral Single-Molecule Imaging
Singe-molecule localization microscopy (SMLM) is a powerful imaging technique that surpasses the diffraction limit and significantly improves spatial resolution over conventional light microscopy, allowing cellular structures and dynamic processes to be visualized at nanoscale. To solve the …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… in modern biological research. Fluorescence microscopy has emerged as an indispensable tool in generating such maps, but common techniques are limited by fundamental physical constraints which render them incapable of simultaneously achieving high spatial and temporal resolution. This thesis …
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Technological Innovation and Integration of Whole Brain Imaging, Olfactory Stimulation, and Correlative Microscopy in Larval Zebrafish
… zebrafish's brain. In this work we show novel light field microscopy algorithms that allow us to reconstruct 3D images from 2D micrographs with improved resolution to enable high-frame-rate recordings of whole-brain neural activity. We describe the designing and building of the first known …
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Advanced image processing methods for 3D high-throughput microscopy
Advances in automated microscopy have led to the generation of vast and complex datasets, bringing bioimaging into the era of large data. This growth demands high-throughput image processing methods. This thesis addresses these challenges by developing efficient feature detection and 3D …
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Three-Dimensional Single-Molecule Localisation Microscopy and Visualisation Techniques
… spread function (DH-PSF) and 3D single-molecule light field microscopy (SMLFM) are techniques which have an increased depth of field (4 µm and 8 µm respectively) and are capable of isotropic resolutions of 25 nm (DHPSF). This makes 3D SR highly compatible to investigate biological processes such …
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Advances in volumetric super-resolution microscopy and single-particle tracking
Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the inherently 3D nature of biological structures, 3D-SMLM techniques---such as the astigmatic and double-helix point spread functions …