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Showing 1 to 5 of 5 for “"Ligation-mediated PCR"”.

  1. Nucleobase deamination as a biomarker of inflammatory processes

    … by coupling their activity with the technique of ligation-mediated PCR. As an extension of the biomarker study, the effects of ONOO⁻ dose and dose-rate on the DNA damage and mutations induced in the supF gene were investigated. The observations suggest that both the dose and dose-rate at which a …

    mit Repository record for Nucleobase deamination as a biomarker of inflammatory processes (opens in a new tab)

  2. Analysis of sequence-selective guanine oxidation by biological agents

    … was also observed in human genomic DNA after ligation-mediated PCR analysis.

    mit Repository record for Analysis of sequence-selective guanine oxidation by biological agents (opens in a new tab)

  3. PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.)

    … was amplified from Kentucky bluegrass by PCR. Using cassette ligation mediated PCR, we amplified four 5’ and five 3’ unknown sequences flanking CH710. The sequence information of these flanking fragments led us to the amplification of three genomic sequences from Kentucky bluegrass by PCR, …

    vt Repository record for PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.) (opens in a new tab)

  4. Epigenetic Determinants of CRISPR-Cas9 genotoxicity

    … role of epigenetic features in influencing Cas9-mediated toxicity. Epigenetics is the study of inheritable changes in gene expression that occur without altering the underlying DNA sequence. Cas9 interacts directly with the genome to introduce double strand breaks at targeted locations. However, …

    cambridge Repository record for Epigenetic Determinants of CRISPR-Cas9 genotoxicity (opens in a new tab)

  5. Fréquence et distribution des dimères cyclobutyliques de pyrimidines suite à une exposition aux UV et évaluation de l’effet protecteur de l’acide sulfonique 2-phénylbenzymidazole-5

    L’exposition aux rayons ultraviolets (UV) du soleil est le principal facteur menant au développement du cancer cutané. Les dimères cyclobutyliques de pyrimidines (DCP) résultant d’une exposition aux UV sont considérés comme les principaux dommages à l’ADN impliqués dans la cancérogenèse cutanée. La …

    sherbrooke Repository record for Fréquence et distribution des dimères cyclobutyliques de pyrimidines suite à une exposition aux UV et évaluation de l’effet protecteur de l’acide sulfonique 2-phénylbenzymidazole-5 (opens in a new tab)