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Showing 1 to 20 of 406 for “"Ligation"”.
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DNA Hybridization and Ligation for Directed Colloidal Assembly
… The salt requirement for DNA hybridization and ligation made it necessary to use slow salt addition through a dialysis membrane to achieve well-ordered colloidal crystals. Well-ordered hexagonally close-packed polystyrene colloid monolayers could be assembled by sedimentation, hybridization, and …
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Thermodynamic simulation of deoxyoligonucleotide hybridization, polymerization, and ligation
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.
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Synthesis of Selenium-Alkylated Selenocysteine Derivatives and Their Use in Native Chemical Ligation, Expressed Protein Ligation and the Synthesis of Dehydropeptides
Finally, an improved, scalable synthesis of Fmoc-phenylselenocysteine is reported. The amino acid was coupled to a model peptide on the solid phase. It was demonstrated that the selenide could be oxidatively eliminated to give a dehydropeptide while still bound to the solid resin. In addition, the …
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Deoxyribozymes That Prepare Branched and Linear RNA by Ligation
… ongoing challenge for deoxyribozyme-mediated RNA ligations has been synthesis of native 3'-5' phosphodiester linkages. Previously, reactions of 5'-triphosphate RNA have provided mostly 2',5'-branched RNA. Here we report two new in vitro selection strategies designed to favor formation of the …
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Stereoselective glycosylation and chemical ligation-applications in oligosaccharide and peptide synthesis.
Stereoselective glycosylation and chemical ligation-applications in oligosaccharide and peptide synthesis.
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DESIGN OF HIGH-YIELDING NON-ENZYMATIC DNA AND RNA LIGATION SYSTEMS
… still exists is how substrates can undergo ligation (i.e. form a covalent bond) in the absence of enzymes. The use of activated species such as imidazole and water soluble carbodiimides have been proposed as means for ligation. However, obtaining the activating agents by a prebiotic route is …
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Studien zur Synthese einer tRNA durch chemische Ligation von RNA-Strängen
In dieser Arbeit wurde eine tRNA durch chemische Ligation von RNA-Strängen synthetisiert. Dazu wurden alle nötigen Methoden für die Synthese, Analyse und Ligation von RNA-Strängen erarbeitet.
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The Effects of Denervation and Arterial Ligation on Calcium Turnover in Bone
Contrary to earlier concepts, it is now recognized that bone takes an active and important part in the general homeostasis of the body. Bone in turn is profoundly affected by changes in the internal and external environment, leading to manifold pathologic conditions, the most common among them …
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Click Chemistry as Efficient Ligation Strategy for Complex Macromolecular Architecture and Surface Engineering
Click-Chemie wurde als Ligations-Strategie für die Synthese von cyclischen Polymeren und zur Oberflächenmodifizierung von großen Mikrokugeln und magnetischen Eisenoxidpartikeln verwendet. Das breite Spektrum dieses universellen und leistungsstarken Instruments im Bereich der komplexen …
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The effects of left hepatic vein ligation on hepatic circulation, function and microanatomy
… was that, in normal dogs, left hepatic vein ligation does not cause severe or permanent liver damage. These findings support a clinical trial of this procedure in patients with patent ductus venosus.
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Site-Specific Incorporation of Biochemical and Biophysical Probes into Proteins Using Expressed Protein Ligation
… semi-synthesis technique Expressed Protein Ligation (EPL). In EPL, recombinant and synthetic polypeptides are joined together via a chemoselective ligation reaction. EPL was originally used to attach synthetic constructs to the C-terminus of recombinant proteins, but is now used to attach …
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Molekularbiologische Gewinnung von RNase 40-124 Fragmenten zur Synthese von einheitlichen Glycoproteinen durch native chemische Ligation
… mit N-terminalem Cys über die native chemische Ligation (NCL) ausgenutzt werden. Diese Methode sollte mit dem Modellprotein Ribonuclease 1-124 entwickelt werden. Zur Synthese der RNase durch NCL wurde das Cys 40 als Ligationsstelle gewählt. Das Fragment 40-124 B mit N-terminalem Cys sollte mit …
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[Beta]-amyloid-mediated nitric oxide release from rat microglia by ligation of the integrin Mac-1
<p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized histopathologically by a loss of neurons and an accumulation of [beta]-amyloid plaques, neurofibrillary tangles, dystrophic neurites, and reactive glial cells. While most previous studies on the neurodegeneration …
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Synthesis of Epidermal Growth Factor by Native Chemical Ligation and Incorporation of Non-Natural Amino Acids
… complete dodecapeptide synthesis. Chemoselective ligation techniques allow the use of unprotected peptide fragments which can eliminate solubility problems associated with fragment condensation. Native chemical ligation results in a peptide bond thus, joining the fragments. Therefore, it was …
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Studying the riboregulatory role of Hfq in Klebsiella pneumoniae using CLASH (crosslinking, ligation and sequencing of hybrids)
… sequencing technique named CLASH (Crosslinking, ligation, and sequencing of hybrids). I performed CLASH in two Kp strains - Ecl8 and Ecl8ΔramR (RamA overexpressor), and established gene regulation mechanisms between transcription and translation through the involvement of Hfq in PTR (Fig A). As …
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A case-control study of menstrual dysfunction occurring in women attending a general practice after tubal ligation
… common during the first two years after tubal ligation. These results could point to factors other than biological factors involved in menstrual dysfunction following tubal ligation.
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A clamp ligation method for point mutational spectrometry : marked increase in scanning range for the human genome
The study of human mutagenesis requires methods of measuring somatic mutations in normal human tissues and inherited mutations in human populations. Such methods should permit measurement of rare mutations in the presence of abundant wild-type copies and should be general to the human genome. A …
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