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Showing 1 to 20 of 148 for “"Ligases"”.
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Lipoic acid protein ligases in Plasmodium spp.
… the biochemical characterisation of lipoic acid ligases and can be adapted to high-throughput screening approaches of inhibitors for these enzymes. Lipoic acid, also known as 6,8-thioctic acid, is an essential cofactor of alpha-keto acid dehydrogenase complexes (KADH) and the glycine cleavage …
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Characterization and evolution of artificial RNA ligases
Enzymes enable biocatalysis with minimal by-products, high regio- and enantioselectivity, and can operate under mild conditions. These properties facilitate numerous applications of enzymes in both industry and research. Great progress has been made in protein engineering to modify properties such …
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Studies on Prokaryotic and Eukaryotic Polynucleotide Ligases
Made available in DSpace on 2014-12-14T07:14:45Z (GMT). No. of bitstreams: 1 7714926.pdf: 4224938 bytes, checksum: 8727bde8d854483297215c784ed8faf6 (MD5) Previous issue date: 1977
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Interaction Between Two E3 ligases, NEDD8ylated Cullin and HHARI
… and its interaction with NEDD8ylated cullin RING ligases.
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Role of Ubiquitin and Ubiquitin Ligases in Retroviral Budding
… bind ALIX, and PPxY domains bind Nedd4-like HECT ligases, a class of ubiquitin ligase enzymes. The functional link between HECT ligases and the VPS pathway has not been defined, but multiple lines of evidence suggest that ubiquitin is generally important for late domain activity. Retroviral Gag …
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Lipoate-protein and biotin-protein ligases of Escherichia coli
… dedicated, highly specific cofactor-apoprotein ligases to generate protein bound lipoyl and biotinyl groups.
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Characterization of Amino Acid tRNA Ligases using the Analytical Ultracentrifuge
… Quaternary structures of amino acid tRNA ligases/synthetases (aaRS) in the native as well as in denatured forms were examined by molecular weight determinations (paper I-IV). Analytical ultracentrifugation, gel electrophoresis, gel chromatography were used in these investigations. The …
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The Role of Two Homologous E3 Ligases In Muscle Physiology
… ubiquitin conjugating enzyme, and E3 ubiquitin ligases. There are an estimated 600 E3 ligases, some of which share high sequence homology; however, the functional significance often remains unknown. FBXL3 and FBXL21 are two homologous E3 ligases that have previously been reported to dictate …
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MECHANISMS OF SUBSTRATE DEGRADATION AND RECRUITMENT BY CULLIN E3 LIGASES
… of the function and regulation of Cullin E3 ligases. We studied the degradation of insulin receptor substrate 1 (IRS1) by the Cullin5 E3 ligase, and found that the basal and signal-induced degradation of IRS1 protein is not dependent on Cullin5. We next investigated the regulation of the …
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Developing as assay to screen inhibitors for various ATP-dependent ligases
DNA ligases (EC.6.5.1.1) are key enzymes that catalyze the formation of phosphodiester bonds at single-stranded or double-stranded breaks between adjacent 5’-PO4 and 3’-OH groups of DNA. These enzymes are essential guardians of genomic integrity and have recently been drawing a lot of attention as …
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Origins and composition of the cullin-RING E3 ligases in trypanosomes
… Cullin-RING is a prominent family of E3 ligases responsible for ubiquitylation of up to 20% of the human proteome. In contrast with the rest of the class, cullin-RINGs perform their function as multi-subunit complexes that comprise substrate adaptors, RINGs for the transference of …
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Regulatory function and interdomain communication of group II biotin protein ligases
… between the groups. Group II biotin protein ligases are characterized by the presence of an N-terminal DNA binding domain that functions in transcriptional regulation of the genes of biotin biosynthesis. Escherichia coli BPL, called BirA, is the best-studied example of how an enzyme can also …
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Sumo and Ubiquitin Ligases Regulate Hepatocyte Nuclear Factor - 1 Beta Transcriptional Activity
… with HNF-1β The SUMO E2 ligase, Ubc9, SUMO E3 ligases, PIAS1 and PIASgamma , and the ubiquitin E3 ligase, Arkadia, were isolated as putative interacting proteins. SUMOylation of transcription factors affects their subcellular localization, intracellular transport, stability, and transcriptional …
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Site-specific labeling of cellular proteins with unnatural substrates of biotin ligases
E. coli biotin ligase (BirA) catalyzes the site-specific ligation of biotin to the lysine within its 15-amino acid peptide substrate (AP). We harnessed the high peptide substrate specificity of BirA to develop a general methodology for site-specific protein labeling with biophysical probes. We …
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Insights into BTB-Cul3 Ubiquitin Ligases from the Structures of SPOP-Substrate Complexes
<p>Cullin-Ring ubiquitin ligases (CRLs) are E3 complexes that specifically recognize substrates through substrate adaptors. In the largest CRL subfamily, Cul3 binds a BTB domain, and a protein-interaction domain such as MATH recruits substrates for ubiquitination. Here we present biochemical and …
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Ligandability of protein-protein interactions and surfaces on Cullin RING E3 ubiquitin ligases
Cullin RING E3 ubiquitin ligases (CRLs) function in the ubiquitin proteasome system by catalysing the transfer of ubiquitin from E2 conjugating enzymes to specific substrate proteins. CRLs are large dynamic multi-subunit complexes that control the fate of many proteins in cells and, therefore, …
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Functional analysis of Shigella encoded IpaH E3 ubiquitin ligases in cell-autonomous immunity
… them a family of twelve bacterial E3 ubiquitin ligases (IpaH) that are structurally unrelated to eukaryotic enzymes. IpaH ligases are injected into the host cytosol via the bacterial type III secretion system (T3SS) to manipulate the host cell and counteract anti-bacterial defense pathways. My …
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Mechanistic Studies of the Activation of Ubiquitin-Conjugating Enzymes by Ring-Type Ubiquitin Ligases
… enzymes, with the help of ubiquitin ligases (E3) transfer ubiquitin onto ubiquitination targets to form isopeptide linkages between the targets and ubiquitin. The function of ubiquitin ligases in the ubiquitination process has not been clearly understood. Recently, it has been shown …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… through the endoplasmic reticulum. These probe ligases will be useful tools for cell biologists interested in studying protein function or protein-protein interactions in the context of living cells.
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Identification and Regulatory Role of E3 Ligases in the Time-Dependent Degradation of the Circadian Factor Period 2
Circadian rhythms are self-sustained, 24h, biological oscillatory processes that are present in organisms ranging from bacteria to human. Circadian rhythms, which can be synchronized by external cues, are important for organisms to adjust their behavior, physiological activity, and metabolic …
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