Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Ligase IV"”.
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Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain
… located in each ventricle of the brain, actively secretes cerebrospinal fluid. By RNA sequencing, we obtained transcriptome data on the choroid plexi from lateral and fourth ventricles of the mouse brain and discovered that they include transcripts unique to each tissue. Transcription factor …
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ANALYSIS OF B LYMPHOCYTES IN MOUSE MODEL LIGASE IV WITH HYPOMORPHIC MUTATION IN VDJ RECOMBINATION ASSOCIATED WITH GROWTH DEFECT
… the DNA-dependent protein kinase , XRCC4 and DNA ligase IV. Rodent cells and mice defective in these components are radiation-sensitive and defective in V(D)J-recombination, showing that NHEJ also functions to rejoin DSBs introduced during lymphocyte development. We have generated a knock-in mouse …
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DNA-PKcs/ Artemis Complex in DNA Double-Strand-Break Repair: Cryo-EM and Biochemical Studies
… and processing, and end ligation. The main objective of my project is to understand the interaction in the complex between DNA-PKcs and Artemis, which is the major nuclease in the step of end synapsis and processing to help clean up the modified DSB ends caused by external factors including …
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The role of linker histone variant in double strand break repair
… as non-homologous end joining (NHEJ). NHEJ, in vivo, requires the KU complex, the DNA dependent protein kinase (DNA-PK) and the DNA ligase IV/XRCC4 (LX) complex. In vitro analysis has shown that several linker histone variants are all phosphorylated by DNA-PK. Depletion of a specific linker …
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Development of CRISPR/Cas9-based gene drive biotechnology in S. cerevisiae
… as a model to develop CRISPR/Cas9-based gene drive biotechnology. The CRISPR/Cas9 biotechnology is a powerful gene editing tool used to modify target genomic sequences in all forms of living creatures including animals, plants, fungi, and bacteria. One potential application of this molecular …
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Development of Natural Cyclic Peptide Inhibitors of XRCC4/XLF Interaction for Radio-Sensitization of Breast Tumor Cells
… called Cernunnos) is responsible for stimulating ligase IV for rejoining DNA ends. A single mutation on the XLF-binding interface of XRCC4 at M61, F106, M59 or D58 has been shown to disrupt its interaction with XLF and thus inhibiting NHEJ. Therefore, it is proposed that small natural cyclic …
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Functional Analysis of the Human Telomeric Protein TRF2
… from telomeres by expression of a dominant negative allele of TRF2 (TRF2ΔBΔM) led dissolution of these structures and appearance of end-to-end fusions visible in metaphase and anaphase cells. Fusion formation was accompanied by the loss of Goverhang, but not by the loss of double stranded …
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
… Artemis and DNA polymerases µ and λ, XRCC4, DNA ligase IV and XLF. The precise molecular mechanism of DNA-PK activation and Artemis processing at the site of a DNA DSB has yet to be elucidated. We have investigated the effect of DNA sequence and structure on DNA-PK activation and results suggest …
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Cryo-EM as a Tool to Study the Diverse Interactome of Ku 70/80 in NHEJ and Beyond
… or repaired incorrectly, they can be drivers for chromosomal rearrangements, cellular senescence, and apoptosis (2, 4). Despite its fundamental role in genomic stability, NHEJ has been implicated in carcinogenesis, tumour progression and radiotherapy and chemotherapy resistance (5). …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
Adenovirus infection activates cellular DNA damage response and repair pathways. The viral E1B-55K and E4orf3 proteins prevent recognition of viral genomes as a substrate for DNA repair by targeting members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as …
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Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
… either the presence or absence of magnesium, to give a 3'-phosphate. Gel filtration chromatography and western blotting codmed that the putative enzyme in human extracts that efficiently processed PG was indeed tyrosyl DNA-phosphodiesterase. When recombinant hTdpl was purified using HiTrap nickel …
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Understanding The Roles of Non-Homologous End Joining and P53 After Dna Damage
… trigger the p53 tumor suppressor network to activate transient cell cycle arrest allowing for DNA repair; if the damage is excessive or irreparable, apoptosis or cellular senescence is triggered. One of the major DNA repair pathway that mends DNA double strand breaks is non-homologous end …
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
… like serine/threonine kinases (PIKK) that collectively regulate the DDR network. Studies have demonstrated these kinases can phosphorylate many of the same substrates, suggesting a significant potential for functional redundancy. However, deficiencies in these kinases have been linked to distinct …
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Caretaker-Gen-Syndrome
… und Charakterisierung nicht-konservativer Spleißmutationen und deren Auswirkungen im ATM-Gen. Ein hoher Anteil der bisher im ATM-Gen identifizierten Mutationen (>350, www.vmresearch.org/atm.htm) stellt Deletionen oder Insertionen direkt an den Exongrenzen dar; viele dieser Aberrationen …
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Distinct DNA Damage Signaling in the Brain Distinguishes ATLD, NBS, and ATR-Seckel Syndrome
… A-T results from homozygous mutations that inactivate the ataxia-telangiectasia mutated (ATM) gene, and typically presents in early childhood as cerebellar ataxia accompanied by ocular telangiectasias, elevated α-fetoprotein levels, and lymphoma predisposition compounded by radiosensitivity. The …