Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"Ligand-binding Assays"”.
-
Comparative analyses of aryl hydrocarbon receptor structure and function in marine mammals
… The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, mediating the expression of a suite of genes in response to exposure to DLC and structurally related chemicals. Species-specific differences in AHR structure can affect an organism’s susceptibility to the …
-
DEVELOPMENT OF ANTAGONISTS TARGETING CHEMOKINE RECEPTOR CCR5 AND THE CHEMOKINE RECEPTOR CCR5 – MU OPIOID RECEPTOR HETERODIMER
… cancer cell lines. In addition, a bivalent ligand containing both a mu opioid receptor (MOR) and a CCR5 antagonist pharmacophore was designed and synthesized in order to study the pharmacological profile of the putative CCR5-MOR heterodimer and its relation with NeuroAIDS. The …
-
Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis
… The interactions between KstR and cognate DNA or ligands have been investigated by multiple biochemical techniques (i.e. electrophoretic mobility shift assay, surface plasmon resonance and intrinsic fluorescence quenching assay). Protein-DNA binding assays have demonstrated that the 21-amino-acid …
-
Developing a selective sphingosine-1-phosphate-5 (s1p5) radiotracer to image oligodendrocytes using preclinical positron emission tomography (PET)
… develop novel Positron Emission Tomography (PET) ligands to specifically bind to oligodendrocytes in the central nervous system in vivo. The ligands target the sphingosine-1-phosphate-5 (S1P5) receptor as a potential marker of oligodendrocyte function with PET. The ligands selected (TEFM180 and …
-
Computational modeling-based discovery of novel classes of anti-inflammatory drugs that target lanthionine synthetase C-like protein 2
… actions. LANCL2 is required for membrane binding and signaling of ABA in immune cells. Direct binding of ABA to LANCL2 was predicted in silico using molecular modeling approaches and validated experimentally using ligand-binding assays and kinetic surface plasmon resonance studies. The …
-
Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy
… T cell Expressed and Secreted), the endogenous ligand of CCR5, were shown to induce different CCR5 signaling pathways and efficiently block viral entry. The peptide analogues are 5P12, which blocks HIV-1 entry but does not induce signaling or receptor internalization; 6P4, which is a non-biased …
-
Atomistic insights into the structure, dynamics, and regulation of integral membrane proteins
… the crucial residues involved in the substrate binding in both outward-facing (OF) and inward-facing (IF) states. Then, through the use of enhanced sampling techniques combining steered MD, string method and umbrella sampling, which can accelerate the sampling of rare events in the simulation, …
-
Development and application of quantitative ligand-binding studies to elucidate mechanistic aspects of pentameric ligand-gated ion channels
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
-
The G protein-coupling specificity of D2-like dopamine receptors
… be expressed in an inducible fashion. A range of ligands displayed agonism at the long isoform of the human dopamine D2 receptor. However, varying degrees of efficacy were observed for individual ligands as monitored by their capacity to load [35S] GTPγS onto each of Gi1, Gi2, Gi3 and Go1. By …