Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Ligand-Receptor Interactions"”.
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Ligand-receptor interactions of Gonadotropin-releasing hormone antagonists
The interactions of selected GnRH agonists and antagonists with the GnRH receptor were investigated using site directed mutagenesis and photoaffinity cross/inking of the receptor with a labelled GnRH analog. With the aim of identifying residues involved in ligand binding, the relative affinities of …
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INVESTIGATING PROTEIN - BILAYER COMPLEXES: A STUDY OF LIGAND - RECEPTOR INTERACTIONS AT MODEL MEMBRANE SURFACE BY USING ELECTRONIC ABSORPTION SPECTROSCOPY AND FLUORESCENCE RESONANCE ENERGY TRANSFER.
… towards the better understanding of protein interactions with the cell bilayer surface. Chapter 1 introduces a brief history on the advent of bilayer systems for cellular research exploration. We presented a literature survey about how liposome systems are used as a complementary technique to …
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Understanding ligand binding, selectivity and functions on the G protein-coupled receptors: A molecular modeling approach
… increasing number of available G protein-coupled receptor crystal structures has enabled utilization of a varied number of computational approaches for understanding the ligand-receptor interactions, ligand selectivity and even receptor response upon ligand binding. The following dissertation …
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Interaction Forces And Reaction Kinetics Of Ligand-Cell Receptor Systems Using Atomic Force Microscopy
… AFM measurement procedures to measure protein interactions on live cells at the single molecular level. These measurements can be interpreted by using proper statistical approaches and can yield important parameters about ligand-receptor interactions on live cells. However, the standard theory …
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Macrophage-mediated resistance mechanisms against MAPK inhibitory by cancer therapeutics
… the full influence of complex intercellular interactions on drug resistance remains a challenge. Here, we combine computation with experiment to investigate the cellular and molecular contributions of the tumor microenvironment to MAPK inhibitor response. First, we employ a computational …
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Understanding tumor cell plasticity in spatial transcriptomics with graph attention networks and walk-based pseudotime analysis
… known correlations, and by leveraging inferred ligand-receptor interactions, we can pinpoint potential drivers of plasticity to test in vitro. PlastiNet effectively generates hypotheses directly from patient-derived spatial transcriptomics samples, offering insights into the cellular mechanisms …
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The role of natural killer cells in control of varicella zoster virus infection
… First, I address the hypothesis that NK cell receptor expression in VZV seropositive patients is different to NK receptor expression in seronegative subjects. By comparing the expression of NK cell receptors in vivo in three cohorts of patients (seronegative, acutely infected, and recovered …
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Cancer Proliferation at the Brain Metastatic Site: A Proteomic Exploration of Inter-Cellular Cross-talk Sustained by Cell-membrane/Secretome Interactions
… cells within the brain is driven by the complex interactions between cancer cells and the brain tumor microenvironment, which is reshaped into a tumor-permissive environment. Therefore, by utilizing mass spectrometry-based proteomic analysis, this study focused on analyzing the secretome and cell …
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Understanding Intercellular Signaling During Lung Injury-Repair
… signals either presented or secreted, known as ligands, to be received by receptors on the surface of another, or in some cases, the same cell. The alveolar region of the lung, the primary region of gas exchange, responds to various types of injuries with different lung repair mechanisms. In …
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Experimental and computational methods for identification of novel fungal histone acetyltransferase Rtt109 inhibitors
… developed and used to pinpoint potential Rtt109 ligand-receptor interactions. 3D-QSAR CoMFA and CoMSIA models that were also derived from the hit series generated in the initial experimental HTS display high self-consistency (r<super>2</super> = 0.985 [CoMFA] and r<super>2</super> = 0.976 …
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A single AKH neuropeptide activating three different fly AKH-receptors: an insecticide study via computational methods
… binding site of a G protein-coupled receptor causes its activation. In this thesis, the structure of Phote-HrTH in SDS micelle solution was determined using NMR restrained molecular dynamics. The peptide was found to bind to the micelle and be reasonably rigid, with an S 2 order …
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Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
… A novel system for the attachment of targeting ligands to the Ad capsid, based on the extremely strong avidin-biotin interaction, is described herein. The genetic insertion of a biotin acceptor peptide (BAP) into the fiber, protein IX, or hexon components of the Ad capsid has resulted in vectors …
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ULTRA SCALABLE METHODS FOR DIFFERENTIAL TESTING OF SPATIAL-OMIC DATA
… this with niche-LR, a method that uncovers ligand-receptor interactions that may underlie niche-specific gene regulation. To ensure scalability, our second innovation is SPARROW, a power-preserving data reduction technique that enables efficient inference across millions of spatial …
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Notch signalling in spinal cord patterning: Crosstalk and fate decisions
… it is likely mediated through differential ligand-receptor interactions. Collectively, this thesis highlights the pleiotropic nature of Notch signalling during neural development. Alongside the classical involvement in progenitor maintenance, Notch signalling also functions to maintain …
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Building and characterising the neuropeptidergic connectome of Caenorhabditis elegans
… neuron gene expression and biochemical data for ligand-receptor interactions. I then used graph theory and machine learning methods to characterise the resulting network. This network is defined by a high density of connections, a clear mesoscale structure, and a large presence of autocrine …
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Nano/Bio-Receiver Architectures and Detection Methods for Molecular Communications
… the various statistics of the stochastic ligand-receptor interactions at the membrane of biological MC receivers. The estimation and detection theoretical analysis of these detection methods demonstrate that even single type of receptors can provide sufficient statistics to overcome the …
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Regulation of natriuretic peptide system activation:effects of spontaneous natriuretic peptide mutations and identification of novel natriuretic peptide receptor phosphorylation sites.
… and GC-B) are transmembrane guanylyl cyclase receptors that mediate the physiologic effects of natriuretic peptides, including reduction of blood pressure and blood volume, stimulation of long bone growth, and regulation of cardiac size. Atrial natriuretic peptide (ANP) and B-type natriuretic …
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Dissecting combinatorial microenvironmental regulation of cell fate and function using a multi-modal arraying platform
… cues can include biochemical elements, as in ligand–receptor binding for cell–cell or cell–extracellular matrix (ECM) interactions, or responses to biophysical parameters, as in cell sensing of substrate material properties. One category of engineered system, high-throughput cell microarrays, …
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Self-assembled Polymeric Nanoparticles for Targeted Delivery of Anticancer Drugs
… toxicity. By covalently coupling targeting ligands, the smart drug delivery systems are capable of targeting specific cell types exclusively through ligand-receptor interactions. The main goal of the project is to create a polymeric nanoparticle drug delivery system from synthesized …
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New Instrumentation and Methods for Analyzing Biological Compounds for Drug Discovery and Discovering Novel Disease Markers
Ligand-receptor interactions mitigate intracellular responses, with abnormal signaling corresponding to different disease states. G-protein coupled receptors currently compose 50% of drug targets, signifying their importance in the pharmaceutical industry. Therefore, monitoring the binding events …
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