Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Library construction"”.
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Enabling sample tracking in and reducing variability in DNA library construction
The Broad Institute is a world leader in genomic sequencing. The Institute gained prominence during the Human Genome Project and has continued to be the nation's leading public laboratory for genomic sequencing projects. The Broad Institute has done this by taking advantage of economies of scale, …
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Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
… Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access and better understanding of cyanobacterial diversity from diverse biotopes. In this investigation, 16s rDNA analysis and DGGE profiling were used to study the diversity of cyanobacteria …
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Studies of Lipoic Acid Related Enzymes
Using a similar strategy of library construction and screening, I also isolated the Vibrio harveyi acyl-ACP synthetase gene and showed that it encoded a 60.4 KDa protein that shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli …
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Scheduling of biological samples for DNA sequencing
… Two consecutive steps are hydroshearing and library construction. Samples arrive randomly into the inventory and are to complete both processes before their due dates. The research project is to decide the optimal sequence of samples to go through these two processes subject to operational …
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DEVELOPMENT OF COMPUTATIONAL METHODS FOR MASS SPECTROMETRY-BASED UNTARGETED METABOLOMICS DATA ANALYSIS
… in MS/MS-based analysis for both spectral library construction and DIA-MS extraction. In “MetaboDIA”, we first assigned putative molecular formulae to MS1 precursor and constructed consensus MS/MS spectral assay library based on the reproducibility with rigorous quality control …
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High throughput screen for cells with high extracellular metabolite consumption--secretion rates using microfluidic droplets
… While many strategies can be deployed in library construction, broad applicability of IME to strain improvement for overproduction of secreted metabolites is severely limited by the availability of high-throughput methods for selecting strains with significantly improved metabolite …
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Enhancing access to public spaces : an evaluation of public libraries and the urban situation in Seoul
… of Seoul through public libraries. The public library has been one of the most Important civic spaces since the invention in the 19th century in the US or UK. While roles of public library are changing due to advances in digital technology, the physical and visible presence of public library …
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A Language for Generic Programming
The past decade of software library construction has demonstrated that the discipline of generic programming is an effective approach to the design and implementation of large-scale software libraries. At the heart of generic programming is a semi-formal interface specification language for generic …
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Comparative analysis of primate hydrogenotrophic microbiota
… Archaea and Desulfovibrionales 16S rDNA, clone library construction of Archaea 16S rDNA and mcrA gene, and quantitative real-time PCR targeting mcrA and dsrA genes. Functionality of methanogens was determined by detection of methane from in vitro incubation of fresh feces. The results showed …
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Photoelectromechanical synthesis of low-cost DNA microarrays
Recent advances in de novo gene synthesis, library construction, and genomic selection for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's cheapest sources of complex oligonucleotide pools. Unfortunately, commercial …
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Identification and bioactivity assessment of an antimicrobial peptide from the defensive skin secretion of the northern leopard frog, Rana pipiens
… the skin secretions of Rana pipiens. After cDNA library construction and gene sequencing of mRNA from skin secretions, a sequence of a mature peptide was identified. After synthesis, identification and purification steps, the peptide sequence was tested for biological activity, including …
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RNA tools for optimization of multi-protein genetic systems
… genetic systems. Typically, this requires construction of a library where variants of parts (e.g. promoters) are tried for each gene, which can be expensive and time-consuming. Here, we present an approach that leverages transacting RNA regulators to explore large gene expression spaces …
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Scalable Methods for In Situ Genomics
… including new chemistries for in situ sequencing library construction, workflows for multimodal sequencing of libraries, and strategies for computational integration of spatial and genetic information. Next, we use IGS to map spatial genome organization in cultured human fibroblasts, validating …
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Identification, Molecular Cloning and Functional Characterisation of Novel Bioactive Peptide Drugs from Amphibian Skin Secretions
… techniques, including mRNA isolation, cDNA library construction, PCR, agarose gel analysis and DNA sequencing were applied. The novel peptide sequences were deduced from cloned cDNAs and then confirmed by using MALDI-TOF mass spectrometry and MS/MS fragmentation sequencing. Finally, three …
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Metagenomics-Based Tryptophan Dimer Natural Product Discovery and Development Pipeline
… of seven steps: 1) soil eDNA extraction; 2) eDNA library construction; 3) homology-based screening; 4) bioinformatics analysis; 5) heterologous expression; 6) characterization of compounds and their biosynthesis; 7) target identification. Using a degenerate primer set that targets the CPA synthase …
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Transcriptional variation in muscle from cattle with alternative NCAPG/LCORL QTL genotypes
… of age. Total RNA was isolated and used for NGS library construction and libraries were sequenced on a HiSeq2500 using single-end chemistry. Sequencing yielded 898 million reads (average >30 million reads/sample) that were mapped to the bovine reference genome (Build UMD3.1). Differential gene …
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Targeted sequencing : single cells and single strand breaks
… at once. However, due to reliance on pooled library construction methods, it is now more difficult to focus on and analyze particular cells of interest, apart from analyzing the library in its entirety. We designed multiplex PCR primers to simultaneously enrich targeted cells from a complex …
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Systematic analysis of genes expressed in the retinal pigment epithelium (RPE) and identification of candidates for genetic susceptibility to age-related macular degeneration (AMD)
… established from an inhouse generated RPE cDNA library. This library was constructed by using the suppression subtraction hybridization (SSH) technique which normalises redundant sequences and ensures enrichment of rare transcripts. In a first phase, 1002 ESTs were sequenced and subjected to …
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The chromatin landscape of barley: gene expression, evolution and epigenetics
… different histone modifications. Following DNA library construction and Illumina sequencing, a bioinformatics pipeline was devised to analyse the sequence data. NGS reads were mapped to a custom assembly of the barley cultivar Morex reference genome sequence before peak calling. Genomic and …
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The development of a high-throughput plasmid assembly pipeline
… for a scalable, high-throughput whole plasmid construction and verification process that can overcome the current limitations of commercial gene synthesis services. Recent progress in molecular technologies and laboratory automation has increased the volume of samples that can be produced, …
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