Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Leucines"”.
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Investigation of the Function and Regulatory Mechanisms of the Mammalian Target of Rapamycin
… in a reporter system. Mutation of the conserved leucines within this export site resulted in a loss of downstream signaling in vivo, despite retention of mTOR catalytic activity. Interestingly, the mTOR 1281--1289L/A mutant showed cytoplasmic staining similar to wild type mTOR, and did not …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… Unexpectedly, mTOR proteins with those conserved leucines mutated to alanines were unable to enter the nucleus. Further investigation revealed that the L982A/L984A and L1287A/L1289A mutations likely induced a global structural change in mTOR, whereas the L545A/L547A mutation directly impaired the …
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The Role of Leucine and Its Metabolite (KIC) in Insulin Signalling and Glucose Transport in L6 Myotubes
… of other amino acids. We also illustrate that leucines metabolite, -ketoisocaproic acid (KIC) inhibits insulin-stimulated glucose uptake at 200 M by 45% concurrent with increased activation of the mTORC1 pathway (p<0.05). Finally, siRNA knockdown of the branched-chain aminotransferase 2 …
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Subunit Interactions of Recombinant HIV-1 Reverse Transcriptase With Mutations at L289
… through hydrophobic interactions with other leucines. L289 is the central leucine of a leucine repeat sequence which resembles a leucine zipper protein-DNA binding motif. Two mutations, leucine to arginine (L289R) and leucine to proline (L289P), were created using PCR mutagenesis. The …