Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"Leptomycin B"”.
-
Regulation of the Intracellular Trafficking of Ajuba by Cell Density and Cell Cycle-Dependent Mechanisms
… into Ajuba variant 1 and its response to Leptomycin B treatment. Specific GFP fusion experiments provided additional evidence for the active nuclear import mediated by the LIM domain. Ajuba's expression levels significantly increased during mitosis, and this surge declined upon mitotic …
-
Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
… but not EGFP alone, after treatment with leptomycin B or after oxidative stress suggests the presence of a previously unidentified nuclear export signal in APE1. Together, these results indicate that the mechanism of nuclear localization of APE1 is complex and regulated via import and …
-
Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
… of Tat degradation. Similarly, use of Leptomycin B (LMB), an inhibitor of Xpo1 mediated nuclear export rescued Tat from degradation. The data suggest that Tat directly or indirectly utilizes Xpo1 pathway of nuclear export. In co-immunoprecipitation experiments, HIV-1 derived Tat was …
-
Subzelluläre Lokalisation des Y-box-Proteins-1 (YB-1) in Mesangialzellen und Charakterisierung der dafür verantwortlichen Proteindomänen
… ( ZLS: AS 1-57, AS 52-146). Experiments using leptomycin B as an inhibitor of the CRM1 dependent nuclear export could reveal a CRM-1 independent export of YB-1. In summary these results document an increased expression and translocation of YB-1 in mesangial cells in the course of …
-
Regulation of PPARgamma Activity by mTOR in Adipogenesis
… of mTOR. As direct evidence, data show that leptomycin B (LMB), a specific inhibitor of nuclear export receptor Crm1 confers the accumulation of nuclear mTOR. Further investigation by altering mTOR's nuclear shuttling activity with exogenous nuclear import and export signals has proved a …
-
Implications of a CALM-derived Nuclear Export Signal for CALM-AF10-mediated Leukemogenesis
… locus. Inhibition of CRM1 with Leptomycin B prevents transcription of <italic>Hoxa</italic> genes in <italic>CALM-AF10</italic> leukemia cells. These findings uncover a novel mechanism of leukemogenesis mediated by the nuclear export pathway and support further investigation of …
-
Zur funktionellen Architektur des Nukleolus in lebenden Zellen
… nicht der Fall, wie insbesondere Experimente mit Leptomycin B gezeigt haben. Diese Droge hemmt den CRM1-abhängigen Kernexport und führte zu einer deutlichen Akkumulation von L10-haltigen Präribosomen im Nukleoplasma von menschlichen Zellen. Schließlich sollte ein neues nukleoläres Protein von …
-
Untersuchung des CRM1-abhängigen RNA-Exports mittels Repräsentativer Differenz-Analyse
… Zellen, die mit dem CRM1-spezifischen Inhibitor Leptomycin B (LMB) behandelt wurden, mit dem mRNA-Export von unbehandelten Zellen verglichen. CRM1-abhängig exportierte mRNAs sollten nach LMB-Behandlung im Kern akkumulieren und aus dem Cytoplasma depletiert werden. Zur Analyse des Kernexports der …
-
Die Bedeutung des Proteins Scarecrow-like 14 in der Regulation der Transkription von Stressgenen
… Kernexport kann durch Behandlung mit Leptomycin B (LMB) inhibiert werden. In mit SCL14-GFP transient transfizierten Tabak BY-2-Protoplasten führt die Behandlung mit LMB zu einer ausschließlichen Lokalisierung im Zellkern. Dies spricht dafür, dass die SCL14-Lokalisierung im Cytosol auf …
-
Identification and characterization of Risp
… from the C-terminal part of Risp. Treatment with leptomycin B, a drug which has been shown to specifically block Crm1 (exportin) mediated export, resulted in nuclear accumulation of Risp-GFP, showing that the nuclear export of Risp, like that of Rev, is Crm1-dependent. Using bioinformatic tools …
-
Etablierung und Optimierung der synthetischen primär-microRNA Technik für funktionale Genomanalysen in Arabidopsis thaliana
… wurde für Wildtyppflanzen beobachtet, die mit Leptomycin B behandelt wurden. Diese Ergebnisse lassen darauf schließen, dass die PABPCs in direkter oder indirekter Weise am Export von mRNPs beteiligt sind, höchstwahrscheinlich über den Exportin1-vermittelten Transportweg.
-
Identification of hepatitis C virus core protein residues critical for the interaction with the cellular DEAD-Box Helicase DDX3 and their functional relevance
… analysed in the presence of the CRM1 inhibitor leptomycin B (LMB). In the absence of LMB, DDX3 was seen to have a diffuse cytoplasmic localisation while a small proportion was also seen in the nucleus. In the presence of LMB however, a build-up of DDX3 was seen in the nucleus, confirming that …
-
The role of human and Drosophila NXF proteins in nuclear mRNA export
… in mRNA export by inhibiting Crm1 with the drug leptomycin B in Schneider cells. Subsequent microarray analysis demonstrated that the inactivation of Crm1 resulted in decreased cytoplasmic levels of less than 1% of all mRNAs, indicating that Crm1 is indeed not a major mRNA export receptor. The …