Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"Lentivirus"”.
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Development of a Lentivirus-mediated Gene Therapy Targeting HIV-1 RNA to Eliminate Infected Cells
… therapeutic constructs were engineered for lentivirus-mediated delivery to HIV-1-infected cells in vitro. I first optimized production of a panel of VSV-G-pseudotyped D4-targeting lentivectors for high infectious titre and low transfer plasmid carryover. I confirmed trans-splicing between …
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The use of lentivirus-mediated gene delivery to investigate the role of the Brain specific transcription factor MYT1L In-vitro and In-vivo
… in which adult mice received microinjections of lentiviruses expressing Myt1l shRNAmir in the dorsal hippocampus. These mice manifested a very subtle and transient increase in anxiety-like behaviour, which normalised with time. Even though the dorsal hippocampus has been only weakly linked to …
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The epidemiology of ovine progressive pneumonia (ovine lentivirus): Diagnosis, risk factor analysis, and production effects in sheep at the United States Meat Animal Research Center
Molecular diagnostic tests to detect ovine lentivirus (OLV) infected sheep were developed and validated and then applied to study the epidemiology and production impacts of natural OLV infection in flocks at the U.S. Meat Animal Research Center (MARC) in Nebraska. Three OLV recombinant proteins …
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Valutazione dell'efficienza, efficacia e sicurezza di vettori lentivirali nel trasferimento del gene CFTR in sistemi modello di epitelio respiratorio in fibrosi cistica
… project is concerned with the realization of a lentivirus working live agent, transferring CFTR WT gene, or SHRNA molecules directed against ENAC subunits; ENAC is a sodium channel hyperactive in cystic fibrosis.
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불멸화 유전자 도입을 통한 베타 세포주 구축
… 세포주 및 세포주 배양 15 2. 췌장 소도 세포 분리 15 3. 불멸화 유전자 도입된 lentivirus 합성 15 4. Lenti SV40T virus와 lenti hTERT virus의 transduction 16 5. Soft agar assay 16 6. Immunocytochemistry 16 7. BrdU incorporation assay 16 8. 단일 세포 선별 18 9. 인슐린 정량 18 10. RT-PCR 18 11. MTT assay 19 Ⅲ. 결과 23 Ⅳ. 고찰 49 Ⅴ. 결론 54 참고문헌 55 …
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Development and characterization of immunogenic genetically engineered mouse models of pancreatic cancer
… antigen-specific T cell response in PDAC, lentivirus encoding the Cre recombinase and a tumor specific antigen (SIINFEKL, OVA[subscript 257-264]) was delivered to Kras[superscript LSL-G12D/+]; Trp[superscript 53flox/flox] (KP) mice. We demonstrate that KP tumors show distinct antigenic …
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A NOVEL METHOD TO INTERFERE WITH GENE EXPRESSION IN MICE
… transgenic knockdown mice using the LentiLox 3.7 lentivirus as a transfer vehicle bearing an U6-promoter dependent shRNA expression cassette. Lentiviruses are a sub-class of retroviruses that have the capability to infect non-dividing post-mitotic cells. Recently, in addition to their use to …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… or integrating viral vectors (e.g., retrovirus, lentivirus). Integration-based approaches risk insertional mutagenesis and oncogene activation, yielding unpredictable, irreversible consequences. Inspired by latent DNA viruses with genomes persisting as episomes, we developed an extrachromosomal …
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Molecular Studies involving the Rev Protein of Caprine Arthritis Encephalitis Virus and Visna Virus.
… (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (<em>gag</em>, <em>pol</em>, and <em>env</em>) and two regulatory genes (<em>rev</em> and <em>tat</em>). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the …
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Rapid Functional Dissection of Genetic Networks Via RNAi In Mouse Embryos
… limitations, I have developed and optimized a lentivirus-based strategy to achieve rapid manipulation of the mammalian epidermis in vivo. Using ultrasound-guided in utero injections of fluorescently-traceable lentivirus particles carrying shRNA or Cre-recombinase into mouse embryos, I …
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Interactions of Mammalian Retroviruses with Cellular MicroRNA Biogenesis and Effector Pathways
… biogenesis mechanisms. In contrast, the lentivirus human immunodeficiency virus type 1 (HIV-1) does not express any viral miRNAs in a number of cellular contexts. Comprehensive analysis of miRNA binding sites in HIV-1 infected cells yielded several viral sequences that can be targeted by …
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Effect of GABRA2 expression in the central nucleus of the amygdala on anxiety and alcohol's anxiolytic capacity in C57BL/6J mice
… surgery for bilateral infusion of GFP-tagged lentivirus targeting Gabra2 or a scramble control lentivirus into the CeA. Following 12-13 days of recovery, mice were assessed for anxiety-like behavior in the elevated plus maze (EPM) naïve or following IP injection of 0, 0.75, or 1.5 g/kg …
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CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS
… fibroblast cell line MSU1.1 to express US28 via lentivirus infection. MSU1.1 cells were transduced with pHAGE-US28-ZsGreen. Fluorescent confocal microscopy was utilized to detect the ZsGreen tag and confirmed the successful transduction of MSU1.1 cells with the US28 vector or empty pHAGE vector. …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
… by viral introduction of the four TFs using lentivirus systems. However, these mechanisms are problematic owing to the possible integration of foreign DNA into cells and subsequent tumorigenic effects. Direct delivery of reprogramming factor proteins via cell penetrating peptides (CPPs) …
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Improving Viral Vectors for Gene Targeting in Gene Therapy
… with some types of virus, in particular, lentivirus. In the first part of this dissertation, this problem is addressed by showing that the addition of caffeine during viral production can increase titer up to 8-fold. Besides lentivirus, other viruses, like Adeno-associated virus (AAV) have …
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A lentiviral screen for novel regulators of IL-7R[alpha] in a pre-B cell line
… are poorly understood. The RNAi Consortium's lentivirus-based shRNA library targeting murine kinases and phosphatases has allowed large scale screening for modulators of IL-7R[alpha][alpha] surface expression. This library provides shRNAs in the pLKO. 1 lentiviral vector targeting 1278 known …
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B-RafV600E에 의한 갑상샘 발암과정에서 Thyroid stimulating hormone (TSH) 의 역할연구
… 4 2. Cloning of B-RafV600E, DUSP6, and c-Myc and Lentivirus Preparation 4 3. Isolation and Culture of Thyrocytes 4 4. Immunohistochemistry and Immunocytochemistry 5 5. Immunoblotiitng 5 6. Analysis of B-RafV600E Mutation 6 7. Immunoprecipitation-Phosphatase Activity Assay 6 8. Measurement of Ras …
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Impact du niveau d'activation de la voie Notch/HES1 dans le maintien du phénotype transformé des cellules pancréatiques cancéreuses
… de gamma-sécrétase et infectées par des lentivirus codant pour des shARNs ciblant de manière spécifique les ARNm de Notch1 ou Hes1. Nos RÉSULTATS démontrent que les cellules BxPC-3 présentent un plus fort niveau d'activation de NOTCH1 qui corrèle avec un plus fort niveau d'expression …
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An ancestral regulatory mechanism underlies Hoxd gene expression in both developing genitals and digits
… in this embryonic structure. We used lentivirus-mediated transgenesis to evaluate the enhancer activity of the identified regulatory elements. While some sequences were able to drive a reporter gene in both digits and genitalia, others were specific for either one of these two …
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Molecular mechanisms of synaptic plasticity
… the underlying molecular mechanisms. Using lentivirus-mediated manipulations of neurogranin levels in hippocampal CAl neurons, we found that increasing neurogranin levels in CAI neurons prolongs the timing window for spike-timing-dependent long-term potentiation (LTP), whereas acute …
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