Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Lentiviral vector"”.
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Lentiviral Vector Engineering for High-Throughput Immune Profiling
The ability to decipher immune recognition is critical to understanding a broad range of diseases, including cancer, infection, and autoimmunity, as well as for the development of countermeasures such as vaccines and immunotherapy. Efforts to do so have been hampered by a lack of technologies that …
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The effect of nfa1 accination against Naeglearia fowleri infection
… of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned with the egfp-nfa1 gene was constructed and characterized for nfa1 DNA vaccination in mice. The expression of nfa1 gene in Chinese hamster ovary cell and human primary nasal epithelial cell …
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Transcriptional targeting of lentiviral vectors to the erythroblastic progeny of hematopoietic stem cells
… in specific cell lineages. HIV-derived lentiviral vectors are very effective in transducing rare, non-dividing stem cell populations without altering their long-term repopulation and differentiation capacity. We developed a strategy for transcriptional targeting' of lentiviral vectors …
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The Epstein-Barr Virus-Encoded BHRF1 miRNAs Regulate Viral Gene Expression in Cis
… difficulties inherent to previously established lentiviral vectors we developed a new lentiviral vector bearing a reverse intron (pTREX) to express the BHRF1 miRNAs at physiological levels. We demonstrated by flow cytometry, Western blot, and TaqMan qPCR that pTREX was better at expressing …
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Efficacy of a Magnetic Drug Delivery System and Development of an Orthotopic Lung Tumor Imaging Model
… cells were genetically modified with plasmid and lentiviral vector constructs to stably express hNIS. Modified tumor cells were then implanted into nude mice to develop two tumor models: the subcutaneous xenograft model and a xenograft orthotopic tumor model. Tumor progression was longitudinally …
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Novel Approaches to Thalassaemia Gene Therapy
… in a lineage-specific manner. Advances in lentiviral vector (LV) technology, together with efforts to incorporate erythroid-specific regulatory elements into gene therapy vectors have yielded some promising results in both in vitro and in vivo studies with a number of clinical trials either …
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A lentiviral screen for novel regulators of IL-7R[alpha] in a pre-B cell line
… This library provides shRNAs in the pLKO. 1 lentiviral vector targeting 1278 known kinases and phosphatases. Analysis of the FACS-based assay identified 38 potential regulators of IL-7R[alpha][alpha] in a pre-B cell line. Subsequent validation of five of the hits confirmed known pathways of …
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Efficacy of Dual Cd19-Cd79B Car In B-Cell Malignancies
… the control of EF1α promoter in a 3rd generation lentiviral vector. The dual CD19-CD79b CAR was composed of CD19 CAR with 4-1BB co-stimulatory domain and CD79b CAR with OX-40 linked by T2A self-cleavable peptide. High transduction efficiency of dual CD19-CD79b CAR was achieved and stable dual CAR …
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p38 MAPK Signalling in Endothelial Apoptosis
… addition to pharmacological inhibition, we used lentiviral vector transfection of EA.hy926 cells to express a dominant negative mutant Flag-p38 MAPK harboring T180A and Y182 F amino acid substitutions. Similarly to the TNF- induced cell death, we found a p38-mediated downregulation of Bcl-xL in …
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Microdevices for Isolating Single Axons for the Study of Axonal Transport in Dopaminergic Neurons
… transduced with a mitochondria targeted DsRed2 lentiviral vector or with a mitochondria specific dye, Mitotracker Deep Red. While both methods successfully labeled the mitochondria, the dye, created significant fluorescent background noise which made measuring mitochondria movement difficult. …
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Defining the biological role of FOXP3 in human CD4+ T cells
… high and constitutive expression mediated by a lentiviral vector, but not fluctuating expression driven by a retroviral vector, was sufficient to generate suppressive cells. Over-expression strategies were also used to characterize a novel splice isoform unique to human cells, FOXP3Δ2 (FOXP3b). …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… Second, we used a doxycycline-inducible lentiviral vector encoding the transcription factors Oct4, Sox2, cMyc, and Klf4 to create a line of naive-like porcine induced pluripotent stem cells (iPSCs). This reprogramming vector was verified first in murine cells, the system in which it was …
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Modeling hematologic malignancies and their treatment in humanized mice
… malignancies in human cells. To that end, a lentiviral vector capable of stoichiometric expression of up to three transgenes in a B cell-specific manner was constructed; in this case, these transgenes included the GFP reporter and the clinically-important oncogenes Bcl-2 and Myc. Enforced …
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TRIB3 as an integrator of metabolic stresses: Role in lung cancer progression
… was determined by RT-PCR and Western blot. A lentiviral vector was used to overexpress TRIB3 in lung cancer cells. A CRISPR-CAS9 construct with guiding sequence matching to TRIB3 gene was used to knock out its expression. Cell proliferation was evaluated using MTS and trypan blue assays. …
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Functional analysis of N-MYC downstream regulated gene 1 (NDRG1) in Oesophageal squamous cell carcinoma
… OSCC cell lines (KYSE30 and KYSE150) by lentiviral vector mediated gene delivery. In KYSE30 cells, although no substantial effects on in vitro cell proliferation and differentiation were observed with altered NDRG1 expression, the ectopic overexpression of NDRG1 was found to be positively …
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Studies of plasticity after neuronal injury
… inhibitory extracellular matrix molecules. Viral vector technology was used to optimise the delivery of ChABC by gene transfer to host spinal cord cells. A lentiviral vector expressing the ChABC gene (LV-ChABC) was delivered to the adult rat spinal cord to achieve long-term delivery of active …
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Characterising changes in pathology and function in clinically relevant models of spinal cord injury and using chondroitinase ABC gene therapy to promote repair
… widespread CSPG degradation was achieved using a lentiviral vector containing genetically engineered ChABC (LV-ChABC). This treatment resulted in significant improvements in injury pathology and functional recovery in the moderate thoracic confusion injury model. LV-ChABC treatment resulted in …
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Towards Optimising Lentiviral Vectors Through Structure Informed Genome Modification
Lentiviral vectors are being successfully used as therapeutic agents in a series of clinical applications of gene therapy, genome editing and cancer immunotherapy. 3rd generation HIV-1 derived lentiviral vectors are produced from 4 independent plasmids. Here, I focus on the transfer vector that …
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