Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Lentiviral Vectors"”.
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Towards Optimising Lentiviral Vectors Through Structure Informed Genome Modification
Lentiviral vectors are being successfully used as therapeutic agents in a series of clinical applications of gene therapy, genome editing and cancer immunotherapy. 3rd generation HIV-1 derived lentiviral vectors are produced from 4 independent plasmids. Here, I focus on the transfer vector that …
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Transcriptional targeting of lentiviral vectors to the erythroblastic progeny of hematopoietic stem cells
… in specific cell lineages. HIV-derived lentiviral vectors are very effective in transducing rare, non-dividing stem cell populations without altering their long-term repopulation and differentiation capacity. We developed a strategy for transcriptional targeting' of lentiviral vectors …
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HIV-1-derived lentiviral vectors pseudotyped with envelope glycoproteins derived from Ross River virus and SemlikI Forest virus
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Efficient Methods for Ex Vivo Generation of Gene-edited Neutrophils
… of progenitors by Cas9 ribonucleoproteins or lentiviral vectors followed by granulocytic differentiation yields uniform populations of gene edited neutrophils in an activation-free manner, allowing the interrogation of individual genes in the neutrophil context. I use lentiviral vectors to …
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Prostate cancer stem cell fate: β-catenin and the Wnt pathway
… Our strategy was to develop and use lentiviral vectors containing short-hairpin RNAs to downregulate β-catenin in prostate cancer cell lines and primary, cultured cells. This work shows that PC-3 prostate cancer cells, infected with β-catenin-shRNA-lentiviruses (PC-3v), displayed a …
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The use of lentivirus-mediated gene delivery to investigate the role of the Brain specific transcription factor MYT1L In-vitro and In-vivo
… yet. In an effort to elucidate its function, lentiviral vectors containing synthetic microRNA-adapted short hairpin (shRNAmir) targeting Myt1l were produced to significantly decrease its expression. The knockdown efficiency of these vectors was corroborated in-vitro in the human neural stem …
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Polyploidy and Mitotic Cell Death are Two Distinct HIV-1 Vpr-Driven Outcomes in Renal Tubule Epithelial Cells
… efficient transduction with VSV.G pseudotyped lentiviral vectors expressing Vpr in the HK2 human tubule epithelial cell line. Using FACS, fluorescence microscopy, and live cell imaging I show that G2 escape immediately precedes a critical junction between two distinct outcomes in Vpr+ RTECs: …
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Experience-dependent dendrite remodeling of GABAergic interneurons in the adult visual cortex
… manner through co-injection of independent lentiviral vectors at a late embryonic or early postnatal age. We demonstrate that excitatory and inhibitory post-synaptic densities can be visualized by tagging fluorescent proteins to PSD95 and Gephyrin, respectively. Finally, we show that the …
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Suppression of telomerase component dyskerin in human haematopoietic progenitor cells
… independent effects. Inducible shRNA knockdown lentiviral vectors targeting either DKC1 or TERC were constructed for transduction into human HPCs. Using this model, it was confirmed that depletion of either dyskerin or TERC effectively suppressed telomerase activity and reduced the proliferative …
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Comparison of wild-type and hotspot mutant p53 interactomes: The hunt for mutant-specific binding partners & Investigation and characterization of natural killer cell responses in genetically-engineered mouse models of non-small cell lung cancer
… in autochthonous models of lung cancer, using lentiviral vectors that initiate tumors and express the NK cell ligand, m157. Using this model, we demonstrate that NK cells become rapidly dysfunctional during tumor progression, but can be stimulated to regain function. We additionally reveal that …
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Molecular Regulation of Opioid Receptor Signaling
… binding site of KOR in vivo; and development of lentiviral vectors expressing KOR which were used to show that KOR expression in the dorsal raphe is sufficient for KOR-mediated analgesia and aversion.
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Modeling Diamond-Blackfan Anemia in the Mouse: Disease Pathogenesis and Evaluation of Novel Therapies
… our mouse models for RPS19-deficient DBA. Using lentiviral vectors we demonstrate that enforced expression of RPS19 cures the lethal bone marrow failure in recipient mice transplanted with Rps19-deficient bone marrow cells. Taking together the generated mouse models provide novel tools to study …
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Understanding the Cancer Resistance Mechanisms of the Naked Mole-Rat
… entire NMR genome. Next, I developed a set of lentiviral vectors to deliver SV40LT-HRASG12V into NMR cells. Using these vectors, I generated 106 different cell lines from 5 different tissues of 11 NMRs and found that, contrary to previous reports, NMR cells are transformed by expression of …
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A NOVEL METHOD TO INTERFERE WITH GENE EXPRESSION IN MICE
… cattle, rats and chickens. Thus, we hoped that lentiviral vectors containing U6/RNAi expression cassettes could serve as a fast and attractive alternative for the generation of mice with reduced expression of specific genes. As a model for this in vivo knockdown approach I chose the hepatocyte …
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Engineering of next-generation chimeric antigen receptors based on alternative binding domains
… single epitope. For this purpose, we synthesized lentiviral vectors containing the coding sequences of these molecules, to subsequently transduce T cell lines. Our research group has recently been working on the design of different CAR-like molecules for the treatment of various pathologies, …
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Novel Approaches to Thalassaemia Gene Therapy
… in a lineage-specific manner. Advances in lentiviral vector (LV) technology, together with efforts to incorporate erythroid-specific regulatory elements into gene therapy vectors have yielded some promising results in both in vitro and in vivo studies with a number of clinical trials either …
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The Epstein-Barr Virus-Encoded BHRF1 miRNAs Regulate Viral Gene Expression in Cis
… difficulties inherent to previously established lentiviral vectors we developed a new lentiviral vector bearing a reverse intron (pTREX) to express the BHRF1 miRNAs at physiological levels. We demonstrated by flow cytometry, Western blot, and TaqMan qPCR that pTREX was better at expressing …
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INDUCED PLURIPOTENT STEM CELLS (IPSC) TO STUDY PATHOMECHANISMS ASSOCIATED TO AMYOTROPHIC LATERAL SCLEROSIS (ALS)
… our results in C9ORF72 iPSC-MN infected with U1-lentiviral vectors. We found that modified U1 snRNA could significantly decrease both the mean number of pathological RNA foci/cell and the percentage of C9ORF72 iPSC-MN forming RNA foci. I also took advantage of iPSC technology to establish a new …