Global ETD Search
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Showing 1 to 20 of 22 for “"Latrodectus"”.
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Sequencing and functional characterization of a Latrodectus geometricus defensin, Lg-defensin
… a new defensin peptide was described in <em>Latrodectus geometricus, </em>Lg-defensin. The full open reading frame was resolved using RACE and <em>de novo</em> assembly, followed by a bioinformatical analysis and phylogenetic alignment to determine conserved sequence patterns and structural …
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
<p>Spiders produce a diverse number of silk proteins that are well-known for their extraordinary mechanical and biological properties. Dragline silk has been the most prominent focus of research because of its exceptional high tensile strength and extensibility. In our research, we have focused on …
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Distribución y frecuencia de latrodectus mactans Fabricio y sus enemigos naturales en El Salvador
En los muestreos de Latrodectus mactans y sus enemigos naturales realizados en los márgenes de las principa1es carreteras de El Salvador se encontró que la Araña tiene una distribución uniforme en todo el país siendo mas frecuentes en las zonas occidental y central. Los enemigos naturales …
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Ontogeny of Venom Use and Venom Composition in the Western Widow Spider Latrodectus Hesperus
… venom and venom use by the western widow spider (Latrodectus hesperus), emphasizing the role of ontogeny. In an introductory paper, I reviewed existing definitions of venom and poison, and refined these by adding a third category of toxic biological secretions: toxungen. These three can be …
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The Prevalence and Effect of Wolbachia Infection on the Brown Widow Spider (Latrodectus Geometricus)
… identified in the Brown Widow Spider (<em>Latrodectus geometricus</em>). This symbiont has not previously been found in this genus, therefore the consequences on the host’s population dynamics are unknown. The frequency of endosymbiont infection can provide insight into the population …
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Isolation and characterization of the tubuliform spidroin 1 promoter from the black widow spider, Latrodectus Hesperus
… TuSp1 core promoter from the black widow spider, Latrodectus hesperus. Using a genomic DNA walking strategy, we have isolated an upstream segment (581 bp) of genomic DNA containing the promoter as well as the first exon of the TuSp1 gene. This upstream regulatory element was able to initiate …
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Structural characterization of spider coating petide [i.e., peptide] 1 and 2 of the black widow spider, Latrodectus hesperus
… found coating various silk fibers in <em>Latrodectus hesperus</em>, the black widow spider.</p> <p>The function of the SCPs is unknown but has-been hypothesized to potentially have antimicrobial properties. We investigated this role and found no significant antibacterial activity of the …
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Characterization of a novel aggregate-gland-derived spider silk protein in Latrodectus Hesperus : from sequence to propsoed function
… silk from the female black widow spider, Latrodectus hesperus, is made of extraordinary biomolecules of nature. Efforts of the scientific community to commercially synthesize silks have become a collaborating, yet competitive race, to characterize the proteins that contribute to its …
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Molecular identification of silk proteins in the gumfooted lines and attachment discs of the black widow spider, latrodectus hesperus
<p>Silks from araneoid spiders have become an active area of research for material scientists, biochemists, and molecular biologists. Mechanical properties of spider silk such as elasticity, tensile strength, and toughness make the manufacturing of silk for medical sutures, body armor, ropes and …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
<p>Spider silk has outstanding mechanical properties, displaying high tensile strength and extensibility. The unique combination of strength and great extensibility make it one of the toughest materials in the world. Of the seven different spider silks, dragline silk, the lifeline silk of the …
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Identification of an aqueous glue protein, SCP-2, and the development of a polyclonal antiserum against the bHLH transcription factor SGSF in Latrodectus Hesperus
<p>Although numerous spider fibroins have been reported, no known silk coating peptides have been discovered. We provide the first biochemical evidence for a spider coating peptide, called SCP-2, found on gumfooted lines, scaffolding joints and egg cases. The presence of this spider coating peptide …
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Part I characterization of MyoR in C2C12 mouse fibroblasts. Part II isolation and characterization of a novel class II bHLH transcription factor from the black widow spider, latrodectus hesperus
<p>PART I</p> <p>The basic helix-loop-helix (bHLH) family of transcription factors are involved in a variety of developmental processes. MyoR is the mouse homologue of the human transcription factor ABF-1 . MyoR is classified as a class II basic-helix-loop-helix transcription factor. In order to …
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Generation of cDNA chips from the black widow spider, latrodectus hesperus, for gene discovery and expression profiling using microarray technology, and molecular characterization of a novel silk glue protein
<p>eDNA microarray technology has generated a tremendous amount of interest among biologists because of its promise to monitor the entire genome on a single chip, thus enabling researchers to have a better picture of the interaction among thousands of genes simultaneously. In the current study, …
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Characterization of the large diameter fibers in egg case silk : identification of a core fibroin, TuSp1, and localization of fibroin-like molecules, ECPs, from the black widow spider, latrodectus hesperus
… spidroin 1 , from the black widow spider Latrodectus hesperus. TuSp 1 was demonstrated to be selectively expressed in the tubuliform gland (the gland suspected for egg case silk production), and examination of the amino acid sequence revealed highly homogeneous repeats (184 amino acid …
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Generation of A L. Hesperus embryonic cDNA library for the isolation of genes involved in early pattern formation
… enable studies of the Westem black widow spider, Latrodectus hesperus, an embryonic eDNA library and a fixation protocol were developed for L. hesperus embryos. The generation of these tools will allow comprehensive analysis of black widow spider development and give insight into whether, and how, …
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Evidence that aciniform silk and minor ampullate silk are major constituents of wrapping silk from the black widow
… type, called wrapping (swathing) silk, from <em>Latrodectus hesperus</em>. My research focused on elucidating the fibroins that make up the swathing silk. Perfonning MS/MS analyses on solubilized wrapping silk fibroins digested with trypsin, we demonstrate that a novel fibroin named AcSp !-like …
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Characterication of aggregate gland silk factor 1
… factor 1 (AgSF1) from the black widow spider, Latrodectus hesperus. After the development of an anti-AgSF1 polyclonal antiserum, we demonstrate by western blot analyses that the AgSF1 protein is highly expressed in the aggregate gland and the protein is localized to the connection joints of …
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The conserved C-terminal domain of spider tubuliform spidroin 1 contributes to extensibility in synthetic fibers
… of synthetic egg case silk fibers spun from the Latrodectus hesperus tubuliform silk proteins, TuSp1 and ECP-2. We provide evidence that recombinant ECP-2 proteins can be spun into fibers that display mechanical properties similar to other synthetic spider silks. We also demonstrate that silks …
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Structural and mechanical analysis of the black widow spider web subjected to stretching, expansion and wind
… on data collected from a member of the species Latrodectus mactans (southern black widow) is tested under three loading conditions: uniaxial stretching, expansion in the three main directions and shearing effects of the wind action. In addition, to determine whether the response of the erratic …
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