Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Lasso Peptide"”.
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Insights into rotaxane formation enable cyclase engineering for lasso peptide diversification
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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Biochemical and structural studies on proteins involved in the production of natural products and degradation of polysaccharides
… synthesized and posttranslationally modified peptide) natural products represent a growing class of compounds. They are biosynthesized from a linear precursor peptide that was translated by the ribosome. A series of post translation modification transform the precursor peptide into the mature …
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Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials.
… natural products within the lasso peptide subfamily. This work led to the discovery of a novel class I lasso peptide, arcumycin, and we validated a conserved mechanism of action for Actinobacteria-produced lasso peptides in targeting lipid II biosynthesis. Our last project …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… and Posttranslationally modified Peptides (RiPPs) are a particularly intriguing class. These compounds begin as a genetically encoded precursor, which is translated into a short (~50) residue peptide. This precursor peptide is then modified by genetically co-located enzymes to …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… synthesized and post-translationally modified peptides (RiPPs) use enzymatic chemistry to diversify simple peptide backbones into architecturally complex and functionally rich natural products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, halogens, …
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Genomics as a tool for natural product structure elucidation
… of streptomonomicin (STM), an antibiotic lasso peptide from Streptomonospora alba, and report the genome for its producing organism. STM-resistant clones of Bacillus anthracis harbor mutations to walR, the gene encoding a response regulator for the only known widely-distributed and …
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Post-translational modifications catalyzed by RiPP and RiPP-adjacent enzymes
… synthesized and post-translationally modified peptides (RiPPs) are, as their name suggests, constructed from ribosomal peptides. The scope of biosynthetic reactions utilized by Nature to transform these peptides into structurally fascinating natural products is astounding. Leveraging these …
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Investigations into the molecular basis of enzyme-catalyzed reactions in natural product biosynthesis
… synthesized and post-translationally modified peptides (RiPPs). The chemical modifications found in these peptide natural products further distinguish each them into specific types. The borosins constitute a class of RiPPs that are characterized by a cyclized N-C structure with methylations on …
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Practical applications of genomics to natural product discovery and biosynthesis
… product gene clusters, and initially focused on lasso peptides, where our application of hidden Markov models, heuristic and sequence motif analysis, and machine learning revealed new scaffolds. This genome-mining approach enabled by RODEO prioritized strains with unique biosynthetic clusters and …