Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 25 for “"Laser-scanning confocal microscopy"”.
-
Quantitative, Competitive PCR and Fluorescent Microscopy Methods for the Study of Batrachochytrium dendrobatidis
… into a quantitative technique. Fluorescent microscopy is a vital tool used to study cellular and molecular biology. DNA and cell wall fluorescent staining of viable Bd observed with laser scanning confocal microscopy is described and should provide the foundation for future studies of Bd …
-
The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana
… wild type at 25° C, but not at 18° C. Laser scanning confocal microscopy was used to examine the localization of auxin transport proteins PIN1, PIN2, PIN3, MDR1, and AUX1 fused to fluorescent proteins. The PIN protein reporters were expressed in fewer cells at lower intensity in …
-
Fabrication of Organized Anisotropic Materials via Evaporative Self-Assembly
… as cell culture scaffolds for hTERT RPE-1 cells. Laser scanning confocal microscopy and scanning electron microscopy were used to image and characterize the aligned cells and live cell imaging was utilized to show that cellular motility can be controlled by highly ordered collagen networks.
-
Out of the ordinary. The materiality of the south-east Scottish Iron Age.
… of analytical methods are employed; including laser scanning confocal microscopy, raman spectroscopy, and residue and isotopic analysis. It becomes evident that the materials studied, despite their predominantly local availability, were invested with meaning in appropriation, making, and were …
-
IMPACT OF PESTICIDES ON THE NEURAL STRUCTURE OF THE HONEY BEE, APIS MELLIFERA
… of the pre-synaptic marker anti-synapsin I and laser scanning confocal microscopy. This permits visualization of synaptic complexes called microglomeruli. Exposure of worker honey bees to fipronil at field-realistic (1 ppb), as well as lower (0.1 ppb) and higher (4 ppb) concentrations decreased …
-
Optical characterisation of the interaction between calcium-silicate based dental restorative materials and dentine
… techniques have been applied; such as tandem scanning and laser scanning confocal microscopy, which were both used with cement labelling and micropermeability tests to evaluate the interfacial morphology and microscopic appearance along with scanning electron microscopy. Additionally, …
-
Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… spermatids using multicolour immunostaining with laser-scanning confocal microscopy (LSCM) and Western blots. Based on significantly increased DDR foci in spermatozoa of smoking men, but lacking DDR foci in response to in vitro challenge with H2O2, an abortive topoisomerase-IIb activity is the …
-
Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… spermatids using multicolour immunostaining with laser-scanning confocal microscopy (LSCM) and Western blots. Based on significantly increased DDR foci in spermatozoa of smoking men, but lacking DDR foci in response to in vitro challenge with H2O2, an abortive topoisomerase-IIb activity is the …
-
Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… 1 day, and 7 days post-transplantation using laser scanning confocal microscopy (LSCM). At 4 hours and 1 day, corneas were imaged for the presence of CMFDA. At day 7, corneas were stained using antibodies to Keratin 3, Ki-67, and the fluorescent probes – PI, and Phalloidin. Cytotoxicity of …
-
Analytical separations using packed and open-tubular capillary electrochromatography
… changes of these coatings were monitored using laser scanning confocal microscopy (LSCM). These changes were discussed in terms of separations using open-tubular CEC (OT-CEC). In addition, the electropherograms obtained from the chiral separation of 1,1’-binaphthyl-2,2’-dihydrogenphosphate (BNP) …
-
Hybrid organic-inorganic nanomaterials for applications at the biointerfaces
… spectroscopy), microscopic (atomic force microscopy, scanning electron microscopy, transmission electron microscopy, laser scanning confocal microscopy) and spectrometric (Time-of-Flight secondary ion mass spectrometry) methods. The research methodology used was interdisciplinary as well …
-
Development of Gold-Magnetite Hybrid Nanoparticles for Advanced Radiotherapy
… were assessed by transmission electron microscopy, x-ray diffraction analysis and ion-coupled plasma optical emission spectroscopy. Colloidal stability and magnetic properties of nanoparticles were determined by dynamic light scattering and alternating field magnetometer. The …
-
Microelectromechanical handheld laser-scanning confocal microscope: application to breast cancer imaging
… investigates handheld instrumentation for laser-scanning confocal microscopy (LSCM) and its applicability to breast cancer detection and subsequent image-guided management. LSCM allows high-resolution mapping of spatial variations in refractive index or tumor marker expression within a …
-
Escherichia coli and Rhizobium leguminosarum response mechanisms to sub-lethal 2,4-dichlorophenoxyacetic acid
… novel combination of methods involving advanced microscopy and metabolomics. Rlv showed an oxidative stress response, but showed adaptive capabilities with changes to specific metabolic pathways and consequent changes to its phenotype, surface ultrastructure, and physical properties during 2,4-D …
-
Development and Characterization of Fatty Acid-Coated Microgels Within Microfluidic Systems
… substrates. Imaging the microstructures with scanning electron microscopy revealed a rough and irregular hydrogel surface, which indicated structural assessment would be challenging since many surface characterization techniques require smooth substrates to acquire depth profiles. The presence …
-
Investigating Tempo-Oxidized Cellulose Nanofiber Lubricating Greases Using Microscopy, Rheology, and Tribology
… systematically characterized using two forms of microscopy, laser scanning confocal microscopy and cryogenic scanning electron microscopy, to understand microstructure networking behavior that ultimately governs their response under mechanical stress. Rheological characterization is performed to …
-
Characterizing uptake, distribution and fate of CdSe/ZnS quantum dots in Synnechococcus elongatus PCC7942
… of uptake and distribution, flow cytometry, laser scanning confocal microscopy and transmission electron microscopy were used. When quantum dots are dispersed into the environment, their imbibition, metabolism, degradation and elimination from cells depends on their surface coating. …
-
Targeted nanoparticle platforms for selective photodynamic therapy of cancer
… Thomsen-Friedenreich (T) antigen. Using laser scanning confocal microscopy a substantial improvement in the cellular internalisation was observed with the jacalin nanoparticle conjugates, as compared to the non-conjugated particles. Similarly, the phototoxicity of the HT-29 cells …
-
Performance and Fouling during Bioreactor Harvesting
… flux and fouling, fouling visualization using confocal laser scanning microscopy, and fouling identification using liquid chromatography-mass spectrometry. </p> <p>For the feed streams studies, filtration of yeast suspensions and Chinese hamster ovary cell culture has been conducted under …
-
Characterisation of chromatography adsorbents for antibody bioprocessing
… with the increasingly adopted techniques of laser scanning confocal microscopy. Experiments are carried out using hIgG to study the static capacity, adsorption equilibrium and dynamic capacity of adsorbents. Other techniques will be used to study the kinetic uptake and desorption rates of …
Page 1 of 2