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Showing 1 to 15 of 15 for “"Laser Scanning Microscope"”.
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DEVELOPMENT OF NON-CONTACT MICROSPHERE CONFOCAL SCANNING MICROSCOPE
The confocal scanning microscope has proven to be a powerful tool to produce high-quality three-dimensional (3D) imaging. However, the resolution of the confocal laser scanning microscope in the visible spectrum is restricted to be ~180 nm and ~500 nm in the lateral and axial direction respectively …
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Fabrication and Characterization of Superconducting Metamaterial Resonators
… microwave measurements as well as Laser Scanning Microscope (LSM) images of the microwave field distributions in the circuit. We compare these various measurements with numerical simulations of the microwave properties of the circuits, including simulated current density and charge …
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Localized adsorption of organic additives during copper electrodeposition
The confocal laser scanning microscope (CLSM) was used in situ during electrochemical experiments to track localized fluorescence patterns of adsorbed organic agents and correlate such adsorption with the changes in surface morphology accompanying electrolysis. In solutions of 5 $\mu$M …
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Investigating transport of charged nanoparticles by multiphoton fluorescence correlation spectroscopy (MPFCS)
… MPFCS system was integrated with a multiphoton laser-scanning microscope (MPLSM) to enable visualization of QD distribution and collagen organization, as well as diffusion measurements. QDs of three different charges by modifying the surface of pre-made CdSe/CdZnS QDs with dihydrolipoic acid …
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In Vivo Assessment of the Forward to Backward Ratio of Second Harmonic Generation By Collagen As Part of a Non-Invasive, All-Optical Biopsy for Skin Cancer
… light was directed into the epidermis using a laser scanning microscope. The SHG light scattered within the tissue, was di↵usely emitted, and collected by the objective. We then acquired a stack of SHG images using a range of confocal aperture diameters and computed the forward-to-backward SHG …
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GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging
… imaged by a standard epi-fluorescence inverted microscope combined withOlympus Scan^R automated image acquisition software; it was demonstrated that the amount of coupled EGFP is proportional to a gray intensity value acquired from fluorescence microscopy imaging. A simple algorithm was …
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Advanced Fluorescence Correlation Techniques to Study Membrane Dynamics
… of diffusion coefficients. Confocal FCS using a laser scanning microscope provides an unprecedented positioning accuracy which enabled us to study, for the first time with FCS, dynamics in bacterial membranes. Scanning FCS with a scan path perpendicular to the membrane plane allows to correct for …
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Comparative neuroanatomy within the context of deep metazoan phylogeny
… (Polychaeta, Annelida). Based upon confocal laser scanning microscope analyses, the distribution of neuroactive substances in the brain is described and the architecture of two major brain compartments, namely the paired mushroom bodies and the central optic neuropil, is characterized in …
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Micro/Nanoencapsulation of Proteins Within Alginate/Chitosan Matrix by Spray Drying
… within the particle was studied by confocal laser scanning microscope with florescent labeled protein. The image showed protein deposition toward the surface of the particles. Total drying time and Peclet number was calculated for the particles and found to be 8.5 ms and 240, which indicates …
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Evaluation of Intestinal Microbial Diversity and a New Antibiotic Regimen in Crohn's Disease Patients
… MAP488 and EC647 probes, respectively. Confocal laser scanning microscope (CLSM) revealed specific signals at 488nm for MAP and 647nm for npEC, indicating probe binding to each bacteria. This was confirmed with hybridization of MAP with EC647 and npEC with MAP488 resulting in absence of signals. …
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In situ Lokalisierung, PGPR-Effekt und Regulation des ipdC-Gens der Azospirillum brasilense Stämme Sp7 und Sp245 bei verschiedenen Weizensorten, sowie endophytische Kolonisierung durch Herbaspirillum sp. N3
… (Triticum aestivum). For this purpose a confocal laser scanning microscope (CLSM) was used, which enabled three-dimesional analysis of bacterial colonization of the root. Especially GFP tagged strains were well suited for this application, as there was no pretreatment or sectioning of the root …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… during the cell cycle and during DNA-repair. Laser-UVA-microirradiation The protein Rad51 is essential for the repair of double-strand breaks (DSBs) via the conservative homologous recombination repair pathway. To test the hypothesis that Rad51 localizes to damaged sites during DSB repair, a …
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The Adhesion Strength of a Plasma Sprayed Silicon Bond Coating on a Silicon Carbide Ceramic Matrix Composite
… grit blasting was evaluated with a confocal laser scanning microscope. The differences and advantages of automated grit blasting compared to manual grit blasting were observed. Most notably was the level of control at high nozzle traverse speeds resulting in reduction of material loss and …
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Frakturbildung in den zahnärztlichen vollkeramischen Materialien auf der Basis von Zirkoniumdioxid
… Weiterführende Analysen erfolgten mit dem Laser Scanning Mikroskop META 510 der Firma Carl Zeiss Jena. Dies ist eine universelle Methode der Werkstoffwissenschaft, um hochgenaue Messungen für Materialien mit relativ rauhen Oberflächen durchzuführen, sowie komplexe Systeme wie Impressionen …