Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Lamp Assay"”.
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Sensitivity of a LAMP Assay for Detection of the Dinoflagellate Amyloodinium ocellatum in Simulated Field Conditions and Freeze Tolerance of the Parasite
… and a loop-mediated isothermal amplification (LAMP) assay were compared for early diagnosis of <em>A. ocellatum</em> in cultured stocks, and the freeze tolerance of tomonts was assessed to determine if frozen wild fish used as fish food can serve as a potential vector for the parasite.</p> …
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Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis
… parasite. Serology (enzyme-linked immunosorbent assay) suffers from a lack of specificity because Strongyloides antibodies are known to cross- react with schistosomal, filarial and other helminthic antibodies in serological tests. Moreover, antibody levels are slow to decline after successful …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION
… on agar plates. There are few rapid assays available that can identify multiple <em>Legionella </em>species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of <em>Legionella </em>in less than one hour. LAMP utilizes …
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The application of loop mediated isothermal amplification for the detection of the sexually transmitted pathogens Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis, at the point of care.
… with loop mediated isothermal amplification (LAMP) and optical detection, capable of detecting the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium and Trichomonas vaginalis in clinical urine and swab samples. LAMP is a novel nucleic acid …
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A Rapid Colorimetric Peptide Nucleic Acid Loop-Mediated Isothermal Amplification Assay for the Detection of the IDH1 Mutation in Glioblastoma
… acid loop-mediated isothermal amplification (PNA-LAMP) method that rapidly detects the <em>IDH1</em> R132H mutation in GBM. PNA-LAMP amplifies target DNA under isothermal conditions with high specificity and speed. The PNA prevents amplification of wild-type <em>IDH1</em> DNA, while allowing …
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Novel and newer nucleic acid amplification tests for the diagnosis of TB
… novel loop mediated isothermal amplification (LAMP) assay on sputum, and that of the semi-automated Xpert MTB/RIF (Xpert) test on non-sputum specimens (bronchoalveolar lavage fluid [BALF], tracheal aspirates, and cerebrospinal fluid [CSF]) from South African patients with suspected TB (the …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering various rabies virus isolates representative sequences from …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering various rabies virus isolates representative sequences from …
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RAPID COLORIMETRIC IDENTIFICATION OF ESCHERICHIA COLI SHIGA TOXINS 1 AND 2, AS WELL AS EAE AND AGGR, USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION FOLLOWED BY PNA/AUNP DETECTION
… called Loop-Mediated Isothermal Amplification (LAMP) to detect different strains of pathogenic <em>E. coli</em>. Common visualization methods of LAMP amplicons include using LAMP reaction buffer containing a pH indictor dye. This method allows for easy colorimetric detection of LAMP amplicons. …
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Loop-mediated isothermal amplification (LAMP) for the diagnosis of human sleeping sickness: towards a point-of-care diagnostic test
… reservoirs of infection. This study focuses upon LAMP (loop-mediated isothermal amplification) as a novel diagnostic for sleeping sickness that may serve to bridge the gap between the need for sensitive, specific molecular diagnostics on the one hand and ‘field-friendly’ diagnostics on the other. …
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Aster Yellows Phytoplasma, Aster Leafhopper And Canola: Development And Application Of Improved Molecular Methods For Pathogen Detection And Genetic Characterization Provide Increased Understanding Of Aster Yellows Disease
… by a loop-mediated isothermal amplification (LAMP) assay. This approach successfully detected AYp in under an hour, and its application could be expanded to a wide range of insect-transmitted pathogens. Disease management can also be improved by identifying and understanding the various …
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Development of a Gram-type Specific Method for Detection of Bovine Mastitis Pathogens by Combining Loop-mediated Isothermal Amplification (LAMP) and Split Trehalase Technologies
… prescribe through two steps: (i) develop PCR and LAMP assay for a point-of-care (POC) amplification of Gram-positive and Gram-negative bacteria and (ii) use a modified amplicon binding split trehalase assay (ABSTA) to detect this amplification by a specific protein-DNA binding and the …
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Microfludic point of care platforms for the detection of viruses, proteins, and nucleic acids
… for portable devices that can perform entire assays with minimal training from the user. This thesis describes a point-of-care platform for the diagnosis of Zika Virus from whole blood using RT-LAMP that can be performed on a portable platform and read with a smartphone camera. An RT-LAMP …
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Microfludic point of care platforms for the detection of viruses, proteins, and nucleic acids
… for portable devices that can perform entire assays with minimal training from the user. This thesis describes a point-of-care platform for the diagnosis of Zika Virus from whole blood using RT-LAMP that can be performed on a portable platform and read with a smartphone camera. An RT-LAMP …
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Point of care platforms for protein and nucleic acid detection
… using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding. A multiplexed microbead immunoassay is developed and validated first for the detection of IL-6. The assay principles are further used to develop microbead based detection for PCT. The final platform …
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Detection and description of influenza a virus transmission in commercial weaned pigs in Illinois, USA
… Loop-Mediated Isothermal Amplification (LAMP) to help address this problem. The first study was performed to develop the primers and test the assay's sensitivity, limit of detection, and specificity using intercalating dye and fluorescence detection in an RT-qPCR machine. Optimized …