Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Lactonase"”.
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Engineering of the quorum quenching lactonase GcL for altered substrate specificity by rational design
… We used the broad spectrum, thermostable lactonase from Parageobacillus caldoxylosilyticus, dubbed GcL, as a starting point of our rational design approach. Previously determined crystal structures of GcL uncovered key positions in substrate binding cleft that interact with the AHL …
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Development of metabolic pathways for the biosynthesis of hydroxyacids and lactones
… was accomplished from levulinate by adding the lactonase paraoxonase I (PON1) to the P. putida hydroxyvalerate production system. Because 4VL was found to exist in a pH-dependent equilibrium with 4HV, the lactonase was expressed extracytosolically in acidic media to achieve significant titers of …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… NDM1 and VIM2, and the N-acyl-homoserine lactonase AiiA. Based on these studies, I was able to identify three clear phenomena that are important in molecular evolution: first, preorganization of the active sites residues is essential for efficient catalysis; second, remote mutations are …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… NDM1 and VIM2, and the N-acyl-homoserine lactonase AiiA. Based on these studies, I was able to identify three clear phenomena that are important in molecular evolution: first, preorganization of the active sites residues is essential for efficient catalysis; second, remote mutations are …
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Metallo-β-lactamase-like enzymes from non-pathogenic organisms: an illustration of functional promiscuity
… MIM-2 share similarities with N-acyl homoserine lactonases (AHLases) and glyoxalase II (GLX-II). Steady-state kinetic assays using a series of lactone substrates confirm that MIM-1 and MIM-2 are indeed efficient lactonases, with catalytic efficiencies resembling those of well-known AHLases. …
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Funktionelle Charakterisierung potentieller Pathogenitätsfaktoren aus Pseudomonas aeruginosa mittels biochemischer und evolutiver Methoden
Es wurden drei ausgewählte potentielle Pathogenitätsfaktoren aus dem opportunistischen Pathogen Pseudomonas aeruginosa biochemisch und funktionell charakterisiert. Die P. aeruginosa Mutante 41D3 trägt eine Transposonintegration in dem Gen Pa5349, welches für eine Rubredoxin Reduktase kodiert. Diese …