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Showing 1 to 20 of 54 for “"Laccase"”.
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Laccase-1 in the pea aphid, Acyrthosiphon pisum (Harris)
Laccases belong to the “multicopper oxidase” family of proteins, and can oxidize o-diphenols and p-diphenols in the presence of molecular oxygen. Laccases have been well characterized in wood-rotting fungi where they appear to play a role in lignin degradation, morphogenesis, and stress defense. …
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Protein engineering of cota laccase by using bacillus subtilis spore display
… are different. In a previous investigation, a laccase (CotA), which is found on the spore coat of Bacillus subtilis, was engineered by directed evolution for improved activity in organic solvents. A CotA variant was identified with a Thr480Ala (T480A-CotA) amino acid substitution after only one …
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Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation
… approaches. The use of enzymes such as laccase as biocatalyst has been recognised as a promising approach for micro-pollutants removal. However, rapid denaturation of the free enzyme and its difficulty in recycling and reuse restrict its wider application, and efficient enzyme …
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Using Esterase and Laccase Enzymes to Derivatize Bioactive Plant Phenolics for Altered Chemistry
… chain acyl group onto the phenolic compound. Laccase enzymes can be used to form phenoxy radicals that can then couple to form larger molecular weight oligomers. Both enzymatic modifications may produce a new antioxidant with altered chemistry. One archaeal esterase (AF1753) from Archaeoglobus …
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Detailed chemical and spectroscopic probes of the multicopper active sites in Rhus laccase
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 1985
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Screening, isolation and characterization of laccase enzymes from Namibian Termitomyces schimperi and Kalaharituber pfeilii
… local Fauna and Flora and little is known about laccase enzymes from Namibia origin hence there is a niche for enzyme studies in this area. This research has qualitatively screened Termitomyces schimperi and Kalaharituber pfeilii fruiting bodies for laccase enzymatic activity using α-naphtnol and …
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Isolierung und Charakterisierung von Kupferhomeostase-Faktoren aus Trametes versicolor und deren Einfluss auf die Laccase-Expression
… des Trans-Golgi-Netzwerks, in dem Laccase mit Kupfer beladen wird,beteiligt sind. Die zwei Kupferpermease-Gene cupA und cupB (copper uptake permease) konnten durch Komplementation der S. cerevisiae Deletionsmutanten ∆ctr1 bzw. ∆cup5 mit einer T. versicolor cDNA-Bank isoliert werden. …
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The Laccase from Micromonospora sp.044 30-1 as a biocatalyst for synthesis of antioxidant compounds
Laccases (EC 1.10.3.2) are blue multicopper oxidases that catalyse a single electron oxidation of various phenolic substrates with an associated four-electron reduction of dioxygen to water. The varied uses of laccase as a biocatalyst can be attributed to its ability to produce a free radical from …
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Structural characterization of the two copper proteins nitrous oxide reductase from Pseudomonas stutzeri and laccase Lcc5 from Coprinopsis cinerea
Obwohl die Reduktion von Distickstoffoxid (N2O) stark exergonisch ist, verhindert eine hohe Aktivierungsenergie eine spontane Reaktion. Wie Distickstoff benötigt N2O ein komplexes Metallzentrum, um aktiviert zu werden. Das einzige bekannte Enzym, welches die Reduktion von N2O zu N2 katalysieren …
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Development of a biocathode system in microbial fuel cells for improved treatment of azo dyes
… catalysts (oxidoreductase enzymes e.g. laccase & microbes). This work investigated the treatment of azo dye (Acid orange 7, AO7) in MFC utilising biological catalysts as replacements for platinum. Various ways of mitigating pH changes in the cathode of MFCs and its effect on laccase …
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Enzymes : the new water/wastewater treatment chemical
… into drinking water supplies. The use of laccase-catalyzed oxidation for removing low concentrations of PPCPs from municipal wastewater after primary treatment is investigated. Oxybenzone was selected as a representative PPCP. Like many other PPCPs, oxybenzone is not recognized directly by …
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Biocatalytic studies of phenol oxidases producing antioxidants
… study reports on the potential application of laccase, obtained from the white rot fungi Trametes pubescens, in the synthesis of organic compounds which are dimers or polymers, and in the development of bioprocesses of potential economic importance. The focus of this study is, particularly, on …
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Enhanced large-scale production of laccases from Coriolopsis polyzona for use in dye bioremediation
… modifying enzymes, a major class of which are laccases (EC 1.10.3.2). Considering the above, three strains of white rot fungi, Coriolopsis polyzona (MUCL 38443), Pleurotus ostreatus (ATCC no. MYA-2306) and Pycnoporus sanguineus (MUCL 41582) were studied for their ability to produce laccases in …
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Function of multicopper oxidases adsorbed on gold nanoparticles /
… are studied. As multicopper oxidases, laccases and human ceruloplasmin are used. The enzymes have been employed in electrochemical systems consisting of gold electrodes covered by gold nanoparticles and enzymes. For the first time direct electron transfer between gold nanoparticle and …
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De Novo Design and Stabilization of β-Sheet Peptides for Biomimicry of ORR Enzymes
… that mimic the structure and function of the laccase enzyme, with a focus on applications in energy conversion technologies. Laccase is of particular interest due to its ability to catalyze the four-electron reduction of molecular oxygen (O₂) to water (H₂O), a reaction essential for the …
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Oxygen reduction electrocatalysis
… for the ORR. The first section focuses on laccase, a multicopper oxidase that catalyzes the four-electron reduction of oxygen to water. Upon adsorption to an electrode surface, laccase is known to reduce oxygen at overpotentials lower than the best noble metal electrocatalysts usually …
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Laccases from actinomycetes for lignocellulose degradation
… sugars. Actinomycetes are known to produce laccases which are able to degrade lignin. Laccase activities were detected in actinomycete strains MS26 isolated from soil collected from the Zambian Copperbelt and DFNR17 isolated from soil collected from a New Zealand farm. Morphological studies …
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Biomembranas formuojančių monosluoksnių ir adsorbuotų biomolekulių struktūros tyrimas virpesinės spektroskopijos metodais /
… tethered bilayer lipid membranes (tBLM) and laccase enzymes at the electrode surface in water and controlled electric potential environments. Water is crucial in maintaining the native state of biomolecules, while the electric methods are widely used in studying such biomolecules. To realize …
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Contribution à la robustesse des traitements fongiques et enzymatiques de contaminants organiques persistants dans des matrices environnementales complexes
… pour la stabilisation d’un extrait brut de laccase pour l’élimination de contaminants dans les effluents municipaux. Le choix était basé sur l’aptitude de sept souches de champignons à éliminer les phénols totaux de l’effluent, ainsi que sur leur capacité à sécréter la laccase. Les deux …
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Development of enzymatic biofuel cell based on carbon nanotube electrodes on porous silicon
… by enzyme catalyst immobilization on the CNTs. Laccase and glucose oxidase (GOx) were used as enzymatic biocatalysts, which were immobilized on the CNT walls and tips using an electrochemical technique. Cyclic voltammetry showed well-defined redox peaks which indicated that the enzyme (GOx and …
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