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Showing 1 to 11 of 11 for “"Lac repressor"”.
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Revisiting Allostery In Lac Repressor
Lac repressor (LacI) is an allosterically regulated transcription factor which controls expression of the lac operon in bacteria. LacI consists of a DNA-binding domain (DBD) and regulatory domain (RD), connected by a linker called the “hinge”. Binding of a small molecule inducer to the RD relieves …
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The Use of High Pressure Fluorescence Spectroscopy to Investigate Subunit Interactions in Oligomeric Proteins (Neurophysin, Lac Repressor)
The application of high hydrostatic pressures (1 atm - 3 kbar) has long been known to lead to the dissociation of oligomeric proteins due to the negative volume change accompanying this process. This technique coupled with fluorescence spectroscopy is used in this thesis in order to determine the …
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Application of the Theory of Elasticity to Modeling of Protein -Dna Interactions
… DNA segments in the crystal structure of the lac repressor-DNA complex. The lac repressor system is used to extensively analyze the parameters and approximations of the model. The capabilities of the model are used to mimic the binding of catabolite gene activator protein (CAP) within the lac …
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Advances in DNA Affinity Chromatography
… columns was characterized. The amount of the lac repressor chimera which eluted from the column was shown to increase with increases in the mobile phase heparin concentration. The elution of the protein was also shown to be dependent on the amount of DNA coupled to the column and more protein …
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A functional analysis of the epigenetic regulator CTCF
… in chromatin, we employed a cell line bearing a lac operator array that can dramatically unfold its normally condensed chromatin when targeted by transcriptional proteins fused to a GFP-tagged lac repressor DNA binding domain. With this system, we demonstrated that CTCF-lac repressor fusions …
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Large-Scale Structure of Mitotic Chromosomes
… copies of a vector containing a 256 copy lac operator repeat and a DHFR gene flanked by the 5' and 3' MARs from the human binterferon gene. Control experiments in which SAR/MAR sequences were not included in the vector were conducted in parallel. Cell clones were isolated which showed …
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Tuning DNA binding and gene expression using zinc finger proteins and engineered promoters
… transcription factors (TFs),2 including the lac repressor, which produces a switch-like “all-or-none” response.3 In this project, we worked to develop a new system for transcriptional control based on tunable synthetic TFs, which are designed to yield programmable linear responses in gene …
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Quantitative all-atom and coarse-grained molecular dynamics simulation studies of DNA
… of a typical gene regulating protein, the lac repressor, which recognizes a particular gene and prevents its expression. First, the large electrostatic charge density of DNA provides an energetic track that guides the repressor’s search for its target binding site. Next, as the protein …
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Multiscale Modeling of Biomolecular Complexes
… dynamics. The method is applied to the E. coli lac repressor, a landmark protein of genetic regulation, that forces DNA into a loop to prevent transcription. The structural dynamics of the complex are revealed by the multiscale simulations, suggesting the mechanisms by which the protein absorbs …
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Construction of an <i>Escherichia coli lac</i> Repressor-Based System to Study Human Cytomegalovirus Gene Expression
<p>An <em>Escherichia coli lac</em> repressor-based system was developed to study the roles of human cytomegalovirus (HCMV) genes during viral replication. To this end, a recombinant HCMV expressing the <em>lac</em> repressor was generated (RV<em>lac</em>), and an HCMV-specific promoter was …
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Stochastic and physical modeling of fundamental biological processes
… in detail two systems: an improved model of the lac genetic switch which includes DNA looping in its gene regulation mechanism, and a spatially resolved, whole-cell model of ribosome biogenesis in Escherichia coli, which we then extend to include cell growth and replication of its genome. For the …