Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Label-free Detection"”.
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External cavity laser biosensor for label-free detection
Optical label-free biosensors serve as a powerful detection tool to analyze biomolecular interactions, and have been widely used in pharmaceutical drug discovery and biochemical sensing. Desirable performance metrics for such sensors include high sensitivity and fine resolution, which are essential …
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Development of label-free detection systems targeting food-borne pathogens
… is a large body of research on the subject of detection of food-borne pathogens, however, the widely accepted current systems are limited by high reagent costs, lengthy time to completion, and expensive equipment. This work has been developed with the goal of focusing on minimizing the time and …
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A photonic resonator interferometric scattering microscope for label-free detection of nanoparticles in point-of-use environments
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-09-01 without embargo terms
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Bioimaging with photonic crystal enhanced microscopy
… photonic crystal enhanced microscopy (PCEM) for label-free detection of the surface attached biological samples. Photonic crystal, supporting guided-mode resonances and providing local optical modes, can enhance the light-matter interaction on its surface and thus used for label-free detection …
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Synthesis and applications of oligonucleotide-bound alginate hydrogels
… use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA …
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Biological detection by means of mass reduction in a suspended microchannel resonator
Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The …
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Multiplexed label-free electrical detection of DNA amplification using field effect transistors
… miniaturized DNA amplification biosensor for the detection and identification of pathogenic bacteria. Using tailored loop-mediated isothermal amplification (LAMP) and field effect transistors, we developed a microchip platform for multiplexed screening of samples querying the presence of multiple …
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DEVELOPMENT OF FREE-LABEL SENSING IN PLASTIC MICROFLUIDIC PLATFORMS USING PULSED STREAMING POTENTIALS (PSP)
This work deals with the development of a new label-free detection technique called Pulsed Streaming Potential (PSP). Its novelty relies on the adaptation of a classical electrokinetic phenomenon (streaming potential) into a tool which can evaluate molecular interplay in label-free fashion. …
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Suspended microchannel resonators for biomolecular detection
Microfabricated transducers enable the label-free detection of biological molecules in nanoliter sized samples. Integrating microfluidic detection and sample-preparation can greatly leverage experimental efforts in systems biology and pharmaceutical research by increasing analysis throughput while …
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Ultra-high-Q Microresonator with Applications towards Single Nanoparticle Sensing
… the theory and experiments for real-time and label-free detection and size measurement of individual nanoparticles and viruses, with a theoretical size limit of R<10 nm. It enables us to cover a large range of virus and nanoparticle sizes of great interest for biomedicine, nanotechnology, and …
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Integrated silicon field-effect sensors and microfluidics for biomolecular detection
… demonstrated. These devices are designed for the label-free detection and recognition of specific biomolecules such as DNA. Label-free methods eliminate the time-consuming and costly step of tagging molecules with radioactive or fluorescent markers prior to detection. The devices presented here …
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Distributed Feedback Laser Biosensor in Microplate Format
… adsorption of biomolecular layers. An assay for detection of the cytokine tumor necrosis factor-alpha (TNF-α) was used to demonstrate the operation of the sensor in the context of label-free detection of a disease biomarker. The demonstrated capability represents an important step towards …
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Aptamer-based nano-scale dielectric sensor for protein detection
The specific detection and precise quantification of protein molecules play an essential role in basic discovery research as well as in clinical practice. In this research work, a novel protein detection mechanism based on nanoscale dielectric sensor functionalized with aptamer probes is developed. …
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Silicon field-effect sensors for biomolecular assays
… real-time readout of molecular interactions. Label-free detection methods can minimize costs in time and resources by obviating preparatory steps necessary with label-based methods. They may further be valuable for monitoring biomolecular systems which are difficult or impossible to tag, or …
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Integrated microfluidics, heaters, and electronic sensors for Lab-on-a-Chip applications
… with the field effect sensing capabilities, for label-free detection of biomolecules such as DNA. The microfluidic valves provide control over reagent flow, and flow rates of up to 1.8nLs⁻¹ have been demonstrated with the on-chip pumps. Initial characterization of the suspended heaters was …
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Functional nanostructured plasmonic materials: fabrication, simulation, imaging and sensing applications
… exciting opportunities for real-time, fully label free forms of chemical/biological detection and field-enhanced applications including surface enhanced Raman scattering (SERS), and photovoltaics. Novel classes of plasmonic crystals fabricated with precisely controlled arrays of subwavelength …
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A micromechanical biosensor with inherently differential readout
… biomolecules in real-time and without the use of labels has significant benefits for systems biology in terms of cost, time and throughput. Cantilever-based micromechanical sensors detect biomolecular adsorption by means of surface-stress-induced cantilever bending. This technique enables …
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Label-free biosensor based upon a vertically emitting distributed feedback laser
… nanorod structure could be used to enhance the label-free detection sensitivity of the vertically emitting distributed feedback laser biosensor (DFBLB). We show that the use of the nanostructured film results in a three-dimensional volume overlap between the DFBLB resonant mode and the region in …
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Studies of biofluid analysis using 2D-IR spectroscopy
… overlaps with the bending mode of H2O, making label-free detection of the protein content in serum challenging. This project demonstrates that 2D-IR can surmount the major obstacle to serum protein analysis as the 2D-IR amide I signature of proteins is shown to dominate that of water. …
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Fabrication and integration of graphene field effect transistors for advanced biosensing platforms
… allowed significant improvement of the detection level of graphene to biomolecules. In this investigation, I realized ultrasensitive, label free detection of DNA with 100,000 times improvement of ultimate limit of detection (LOD) compared to a flat graphene device.
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