Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"LY294002"”.
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SYNTHESIS AND SCREENING OF PI3K INHIBITOR PRODRUGS FOR TREATMENT OF PROSTATE CANCER
… identified as PI3K inhibitors, e.g., wortmannin, LY294002, and, ZSTK474, but all have low selectivity. We have addressed the specificity of current PI3K inhibitors by developing PSA (prostate-specific antigen)-activated analogues of known PI3K inhibitors LY294002 and ZSTK474.
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Roles of Growth Hormone, Insulin-Like Growth Factor I, and Sh3 and Cysteine Rich Domain 3 in Skeletal Muscle Growth
… by adding to the culture medium rapamycin, LY294002, and PD98059, which are specific inhibitors of the signaling molecules mTOR, AKT, and ERK, respectively. The effectiveness of these inhibitors was confirmed by Western blotting. Proliferation of bovine myoblasts was stimulated by IGF-I, and …
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Characterising the role of actin and PI (3) kinases in endocytosis in the malaria parasite Plasmodium falciparum
… stabilises actin filaments, and Wormannin and LY294002, which inhibit PI 93) kinase, were used to study actin disrupting and PI (3) kinase inhibiting drug effects on haemoglobin endocytosis and transport vesicle trafficking within the malaria parasite Plasmodium falciparum.
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Molecular Mechanism of Tetraspanin CD9 Mediated Cell Motility
… kinase (PI-3K) inhibitors, wortamannin and LY294002 inhibited CD9 promoted cell motility in Chinese hamster ovary (CHO) cells. In contrast, inhibitors targeting protein kinase C or mitogen-activated protein kinase had no effect on CD9 driven CHO cell motility. Furthermore, inhibition of …
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U0126, MEK1/2 inhibitor, Induces Adhesion Molecules Expression in Human Blood Cells
… the effects of various inhibitors such as LY294002 (an inhibitor for PI3Kinase), trolox (antioxidant), rottlerin (an inhibitor for PKC-δ), and SN50 (an inhibitor for NF-kB) on U0126-induced adhesion molecule expression. Adhesion molecule expression was attenuated by rottlerin and trolox. In …
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Mechanismen der Urocortin-II-induzierten Stimulation der NO-Produktion in isolierten Kaninchen-Ventrikelmyozyten
… 3-Kinase (PI3K), Wortmannin (0,3 µM) und LY294002 (10µM) nahezu vollständig aufgehoben. Konfokale Bildgebung der zellulären NO-Produktion und der fraktionellen Verkürzung zeigen einen Anstieg des [NO]i und der fraktionellen Verkürzung mit einem ähnlichen Zeitverlauf. Die NO-Produktion …
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Brain Inflammation Regulated by PINK1
… degradation, and I found that an Akt inhibitor, LY294002, increased the expression of pro-inflammatory cytokines. These results suggest that PINK1 deficiency enhances the expression of pro-inflammatory cytokines through reduced STAT3 and Akt activation, enhanced IkB degradation in response to …
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Effect of Prostaglandin E2 on the Expression of Adhesion Molecules in Mouse Cerebrovascular Endothelial Cells
… the separate experiment using a PI3K inhibitor, LY294002, constitutive active (CA) and dominant negative (DN) Akt constructs. PGE2 induced the activation of NF-κB, critical regulator of ICAM-1 expression and NF-κB inhibitors blocked PGE2 and dbcAMP-induced ICAM-1 expression. Interestingly, loss …
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RESVERATROL INHIBITS PROSTATE CANCER GROWTH AND METASTASIS BY TARGETING AKT/MICRORNA-21 PATHWAY
… which were reduced by both resveratrol and LY294002, a known PI3-kinase inhibitor. <italic>MiR-21</italic> expression in these cells appears to be dependent on Akt, as LY294002 reduced the levels of <italic>miR-21</italic> along with a concurrent increase in PDCD4 expression. These in vitro …
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Identifying a role for Ciz1 in the DNA damage response
… is inhibited by both wortmannin and LY294002, implicating DNA-PK as the responsible kinase. More traditional methods including immunofluorescence and RT-PCR were used to characterise the effects of DDR signalling upon endogenous and overexpressed Ciz1. The resulting data show that …
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Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target
… (P<0.01) in SAECs, and this was attenuated by LY294002, 3-MA, HCQ, and knockdown of ATG5 and Beclin-1 (in A549 cells) (P<0.01). Conclusions and clinical relevance: Autophagy could play a role in the pathogenesis of severe asthma. Autophagy modulation may be a novel therapeutic target for …
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Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
… is not neutralized by the PI(3)-kinase inhibitor LY294002, PI(4,5)P2 ligands, such as neomycin, a PI-transfer protein that can remove PI from membranes, and PI(4,5)P2, PI(3)P and PI(4)P antibodies etc. In whole-cell recording, non-SG fusion is strongly reduced by osmotically-induced cell swelling, …
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Alpha v beta 5 and related receptors in human B lymphocyte development
… RKC motif was abrogated by the PI3K inhibitor LY294002 and western blotting revealed that phosphorylation of Akt was enhanced by stimulation with RKS containing peptides. Among CD23’s receptors is the integrin αvβ3. This integrin can form a signalling complex with CD47. Ligation of CD47 by …
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Redox mechanisms in retinal cells
… I (a protein kinase C inhibitor) and LY294002 (a phosphionositide 3-Kinase (PI3K) inhibitor). These results suggest that Mueller cells may be more resistant to oxidative stress than other retinal cell types and that SFN utilises the Nrf2 pathway to amplify the anti-oxidant response in …
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Evaluation of inhibitors of histone deacetylases as potential neurotrophic agents
… inhibitors of MEK1/2 (PD98059/U0126) PI3K (LY294002) and TrkA (GW441756) and phosphorylation of target kinases. Associated factors namely, acetylation of histones (H3/H4) and α-tubulin, role of cAMP, and cell- cycle analysis with flowcytometry were also studied. </p> <p>Vorinostat, a pan …
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Mechanisms associated with Staphylococcus aureus and Acanthamoeba Interactions, and Therapeutic applications"
… studied through the use of inhibitors such as LY294002 and wortmannin, while the involvement of protein tyrosine kinase was studied through genistein and protein tyrosine phosphatase by sodium orthovandate (Na3VO4). The role of serine proteases of Acanthamoeba was investigated by using its …
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The Role of Igf-1 and Il-4 in Myeloid Progenitor Cell Survival
… of these cells with either wortmannin or LY294002, two potent inhibitors of PI 3-kinase activity, abrogates the ability of IGF-I or IL-4 to maintain the expression of Bcl-2 in FDCP cells. As expected neither inhibitor affected the ability of IL-3 to maintain Bcl-2 expression or inhibit …
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Transcriptional Regulation of human Plasminogen Activator Inhibitor-1 Gene Expression by Insulin-like Growth Factor-1, Insulin and Upstream Stimulatory Factor-2
… E-Boxen E4 und E5. Die Hemmung der PI(3)K durch LY294002 oder der ERK1/2 durch U0126 reduzierte die HIF-1 alpha-Proteinspiegel, während beide Inhibitoren zusammen den IGF-1 Effekt auf HIF-1 alpha vollständig aufhoben. Transfektionen in HepG2-Zellen mit dominant-negative PDK1 oder TRB3, dass …
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Role of the LPA2 Receptor in Protecting Against Apoptosis
… the MEK inhibitor PD98059 and the PI3K inhibitor LY294002. Pertussis toxin partially abolished OTP-induced ERK1/2 and apoptotic protection. In RH7777 cells lacking LPA receptors, OTP selectively protected LPA<sub>2</sub> but not LPA<sub>1</sub> and LPA<sub>3</sub> transfectants. In C57BL/6 and …
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Dock180/Elmo proteins are critical regulators of phagocytosis,macropinocytosis and chemotaxis in Dictyostelium
… inhibited in presence of the P13K inhibitor LY294002. doCkAnu#' dockBn1 dockA/8""0 cells all show reduced actin polymerization upon CAMP stimulation. dockC"'l' cells also show poor chemotaxis to CAMP. However there is no obviously defect in chemotaxis, macropinocytosis and phagocytosis for …
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