Global ETD Search
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Showing 1 to 9 of 9 for “"LOPIT"”.
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… Tagging after Differential ultraCentrifugation (LOPIT-DC). LOPIT-DC can be employed in the future to expand the comparison between distinct states of pluripotency towards an additional dimension, specifically by interrogating the role of protein subcellular localisation dynamics in regulating …
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… Tagging after Differential UltraCentrifugation (LOPIT-DC) method. Over 4000 proteins were identified, of which ∼40% were mapped to a specific organelle under each condition and ∼10% of proteins were predicted to translocate in response to insulin. Novel insulin-responsive protein translocation …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… Tagging with differential centrifugation (LOPIT-DC), was coupled with a semi-supervised Bayesian mixture model to address these technical limitations and screen for protein localisation events in an untargeted and cell-wide manner. First, protein expression was measured within the lung …
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Nutraceutical modulation of lipotoxicity and the development of a tool to monitor palmitate induced sub-cellular dysfunction.
… of organelle proteins by isotopic tagging (LOPIT) technique. Organelles are separated based on their densities and proteomic and lipidomic LCMS open-profiling is used to quantify proteins and lipids abundances across the differential centrifugation fractions. Known organelle marker proteins …
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Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… Tagging with Differential Centrifugation (LOPIT-DC) technique combined with downstream analysis using the Bayesian ANalysis of Differential Localisation Experiments (BANDLE) algorithm. Subcellular proteome maps were generated for mock transfected and rAAV6.2-producing HEK293-VP 2.0 cells at …
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Discovery and functional analyses of new components of the apical complex and the pellicle of Toxoplasma gondii
… proteins based on the new proteomic method LOPIT and to identify if MORN domain-containing proteins will comprise further elements of the pellicle beyond those already known. The locations of six novel microneme proteins (MC1-MC6) and five MORN domain- containing proteins (MORN2-MORN6) were …
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Bayesian Methods for Spatial Proteomics
… of Organelle Proteins by Isotope Tagging (LOPIT) and Protein Correlation Profiling (PCP). Now these techniques have been redeveloped and a typical experiment can accurately measure thousands of proteins per experiments, whilst also providing information on (at least) a dozen subcellular …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… of Organelle Proteins by Isobaric Tagging (hyperLOPIT); a high-resolution mass spectrometry-based technique, the first, cell-wide proteomic map of the CHO-K1 host is detailed (Chapter 5). This has provided for the unprecedented spatial resolution of thousands of proteins distributed within the …
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Interrogations of protein and RNA: localisation, interactions and dynamics
… iodixanol containing gradients, termed “MultiLOPIT”, is presented, which provides high-resolution organelle resolution for comparative applications in different cell states or conditions. In Chapter 4, characterisation of the dynamics of protein and RNA-binding proteins, post-translational …