Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"LNCaP cells"”.
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Androgen Receptor and Prostate Cancer Cell Heterogeneity
… A more specific postulate is that PCa cells expressing AR (i.e, AR<sup>+</sup>) and PCa cells expressing little AR (i.e, AR<sup>-/lo</sup>) possess intrinsically distinct biological and tumorigenic properties as well as sensitivities to AR-targeting therapies. Here I tested my …
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Evaluation of therapeutic effect of IKK epsilon SiRNA on breast cancer cells and development of a peptide-based SiRNA delivery system
… potential of IKKε siRNA on human breast cancer cells. Eight siRNAs targeting different regions of the IKKε mRNA were designed, and the silencing effect was screened by quantitative real time RT-PCR. Silencing of IKKε in human breast cancer cells resulted in decrease of focus formation potential …
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Investigations on the effects of Typha capensis on male reproductive functions
… from T. capensis rhizomes. Part 1: TM3-Leydig cells and LNCaP cells incubated with different concentrations of crude aqueous extract of T. capensis rhizomes (0.01, 0.02, 0.1, 1, 10 and 100 µg/ml) and control (without extract) for 24 and 96 hours, after incubation. The following parameters were …
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Testing Whether MRP4 (a cAMP Efflux Pump) and the Beta 2 Andrenergic Receptor (an Upstream Regulator of cAMP Signaling Pathways
… that can efflux the second-messenger, cAMP, from cells. MRP4 has a PDZ interacting motif at its carboxy terminal end through which it binds to scaffolding proteins NHERF1 and PDZK1. Previous studies have shown that PDZK1 serves as a scaffold physically coupling MRP4 with the cystic fibrosis …
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CaV1.3 Ion channel expression and hormone therapy in prostate cancer
… CaV1.3 was overexpressed in AR-positive PCa cells but appeared to be localised within subcellular compartments. Treatment of LNCaP cells with AR antagonist, Enzalutamide resulted in the re-localisation of CaV1.3 to the plasma membrane where calcium imaging techniques highlighted the presence …
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Metabolomic and metabolomic flux studies of the effects of sphingosine kinase inhibitors on prostate cancer cells using high-resolution mass spectrometry as an analytical technique
… sphingosine kinase inhibitors in prostate cancer cells in order to establish the role of SK1 and SK2 in this cancer. Previous studies have shown that the SK1/2 inhibitor, SKi (2-(phydroxyanilino)- 4-(pchlorophenyl)thiazole) inhibits aerobic glycolysis (Warburg effect) and this might contribute to …
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Cell Toxicity and Uptake of RRR-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (TPGS) by Various Cell ines <em>In Vitro</em>.
… Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or α-TS at concentrations ≥ 12.4 μM suggest that α-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of α-TS. For both cell …
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Characterization of Gene Expression During Adenosine 3':5'-Cyclic Monophosphate Induced Neuroendocrine Differentiation in Human Prostatic Adenocarcinoma
The LNCaP cell line is a versatile and useful model that is suitable for the study of human prostate cancer in vitro. The elevation of LNCaP intracellular cAMP levels through the addition of membrane permeable cAMP analogues, phosphodiesterase inhibitors, adenylate cyclase activators, or components …
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Phage Display Library Screening For Psa-/Lo Prostate Cancer Cell-Binding Peptides
… and developing therapeutics that target the cells responsible for driving PCa and mediating therapy resistance. My master thesis research employs a phage display library screening technology aiming to identify peptides that preferentially home in to undifferentiated PCa cells, which our lab …
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Leukemia/Lymphoma Related Factor in Human Prostate Carcinoma
… EGF on the expression of LRF/Pokemon in prostate cells.|The expression of LRF/Pokemon was examined in resected tissue specimens from the patients with BPH and prostate adenocarcinoma by immunohistochemistry and immunofluorescence using antibody against LRF/Pokemon. LRF/Pokemon was expressed in …
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Investigating PLEKHS1 adaptor function in PI3K signalling and human cancer biology
… PLEKHS1 adaptor protein function *in vitro*, LNCaP prostate cancer cell line is used due to its PTEN-null status and PI3K pathway hyperactivation. Transient over-expression of PLEKHS1 in LNCaP cells generates a robust PIP<sub>3</sub> response via YxxM-dependent interactions with endogenous …
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Preservation of Bioactive Compounds in Blueberry Fruit
… the growth of hepa-1c1c7 mouse liver cancer and LNCaP human prostate cancer cells without cytotoxicity, and inhibited adhesion of E. coli bacteria implicated in urinary tract infections. In an experiment comparing antiproliferation activity of wild and cultivated (Vaccinium corymbosum L.) …
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“Differential regulation of PTHrP gene expression by 1,25(OH)2D3 in prostate cancer cell lines”
… protein (PTHrP) is expressed by prostate cancer cells. Since PTHrP enhances the growth and osteolytic potential of prostate cancer cells, it is important to control PTHrP expression in these cells. 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) downregulates PTHrP expression in a variety of human cell …
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Regulation Of Membrane-Type 1 Matrix Metalloproteinase In Prostate Cancer
… expressed in nonmalignant primary prostate cells, moderately expressed in DU-145 cells, and highly expressed in invasive PC-3 and PC-3N cells. Using MT1-MMP promoter reporters and mobility shift assays, we show that Sp1 regulates MT1-MMP expression in DU-145, PC-3, and PC-3N cells and in …
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Cell surface receptor specific delivery of short interfering RNAs via bivalent aptamer-siRNA transcripts or covalent antibody-siRNA conjugates
… potential to selectively eliminate neoplastic cells by the silencing of genes that are crucial for cell growth and viability. Unlike small molecule drugs siRNAs are not passively taken up by target cells. Therefore the efficient intracellular delivery of functional siRNAs is one of the biggest …
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Regulation of Cell Invasion by the Podosome/Invadopod Protein Tks5
… and their impact on the invasive phenotype of cells. The podosomes of macrophages enable tissue invasion during immunosurveillance and wound healing. Using model THP1 macrophages, my studies have shown that Tks5 can directly control podosome-associated matrix degradation and invasion through …
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The effects of snake venoms as cytotoxins on selected prostate cancer cell lines
… panel of prostate cancer cell lines (DU145, PC3, LNCaP) and a non-malignant prostate epithelial cell line (PNT2A). The cytotoxicity activity of each venom was initially assessed using the SYTOX Green assay. SYTOX Green is a fluorescent nucleic acid indicator dye that can be used as a marker for …
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ACETYL COA CARBOXYLASE 1 IN PROSTATE CANCER AS A CHEMOTHERAPEUTIC TARGET
… sources of fatty acids. Androgen-dependent LNCaP cells tend to metabolize endogenously supplied fatty acids while androgen independent DU145 cells preferentially metabolize exogenously supplied fatty acids. Pharmacological inhibition of ACC by a non-isoform specific inhibitor reduced the …
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Novel genotoxins that target estrogen receptor- and androgen receptor- positive cancers : identification of DNA adducts, pharmacokinetics, and mechanism
… molecules capable of selectively killing tumor cells that aberrantly express steroid hormone receptors. Many human breast cancers express high levels of the estrogen receptor (ER), and most prostate cancers express the androgen receptor (AR). We reasoned that the potential genotoxic effect of …
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A metabolic perturbation by U0126 identifies a role for glutamine in resveratrol-induced cell death
… castration-resistant human prostate cancer C4-2 cells are more sensitive to resveratrol-induced apoptosis than isogenic androgen-dependent LNCaP cells. Inhibiting downstream pro-survival signaling with the MEK inhibitor U0126 rescued the C4-2 cells from resveratrol-induced death, however other …
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