Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"LGR5"”.
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Lgr5 Regulation of Stat3 Signaling and Drug Resistance In Colorectal Cancer
<p>LGR5 Regulation of STAT3 Signaling and Drug Resistance in Colorectal Cancer</p> <p><em>Tressie Alexandra Capri Posey </em>B.S.</p> <p>Advisory Professor: Kendra Carmon, Ph.D.</p> <p>The greatest difficulty in treating colorectal cancer (CRC) is the development of drug resistance which leads to …
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Development and characterisation of a novel chimeric antigen receptor against LGR5
… repeat-containing G-protein coupled receptor 5 (LGR5), a target gene of the Wnt signalling pathway, is lowly expressed in some stem cell niches and overexpressed in multiple cancers, including pre-B acute lymphoblastic leukaemia (pre-B ALL), hepatocellular carcinoma (HCC) and colorectal cancer …
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Cochlear hair cell regeneration from neonatal mouse supporting cells
… with the supporting cell markers Sox2 and Lgr5, we show that Lgr5-positive inner pillar and 3rd Deiter's cells in gentamicin-damaged organs of Corti from neonatal mice give rise to new hair cells following treatment with a Notch inhibitor. These new hair cells are generated primarily …
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Defined populations of inner ear progenitor cells show limited and distinct capacities for differentiation into hair cells, neurons, and glia
… of cochlear supporting cells positive for Lgr5 (Lgr5+ cells) was limited to differentiation into hair cell-like cells but not neuron-like cells. In contrast, glial cells positive for PLP (PLP1+ cells) from the auditory nerve differentiated into multiple cell types, with properties of …
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Growth Factors Up-Regulate Epithelial Stem Cells: Treatment Strategies for Colorectal Cancers
… against colon cancers. Since both DCAMKL-1/LGR5 have been reported as colonic CSC markers, the possible phenotypic/proliferative differences between DCAMKL-1+ve and LGR5+ve human colon CSCs was examined. Results in chapter 4 suggest that DCAMKL-1+ve cells are significantly more proliferative …
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Specific forms of TCF7L2 protein define the Wnt pathway dependent genetic program
… increase its affinity for its cognate receptor LGR5. LGR5 expression is specific to stem cells whereas the closely related LGR4 receptor is expressed by more differentiated cell types in the intestinal epithelia. My engineering was successful, producing a mutant version of R-spondin1 that …
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Targeting the intestinal stem cell niche through energy and metabolism signaling, for colorectal cancer chemoprevention using aspirin and metformin
… reduced transcript levels of stem cell markers LGR5 and CMYC, and inhibited mTORC1 downstream target phosphorylation levels. Utilizing 3-dimentional (3D) sphere formation assays revealed that aspirin and metformin also reduced stem cell function in the HCT116 cell line. Drug combination showed a …
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SIGNALLING TISSUE RENEWAL AND CRYPT SURVIVAL IN THE HUMAN COLONIC EPITHELIUM AND BARRETT’S OESOPHAGUS
… and BMP pathways. A population of intestinal Lgr5/OLFM4+ stem cells were found to be interspersed between goblet cells at the base of the crypt. Exogenous Wnt signals maintained Lgr5/OLFM4+ stem cells, whilst BMP and TGFβ inhibited and caused complete loss of stem cells. Wnt signals also …
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Ontogeny and biological function of epithelial cells in the chicken yolk sac and small intestine
… (PepT1 and SGLT1), intestinal stem cell markers (Lgr5 and Olfm4), and avian beta defensins in the chicken yolk sac and small intestine during the embryonic and early posthatch stages. RNAscope in situ hybridization was used to identify the distribution of cells expressing PepT1 mRNA in both the …
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Outlining a balance-point model of homeostasis in the small intestine of broiler chickens
… Repeat Containing G Protein-Coupled Receptor 5 (Lgr5) decreased during the post-hatch period while Olfactomedin 4 increased post-hatch. Both stem cell genes were expressed within the intestinal crypt, though prior to hatch Lgr5 was expressed in the lamina propria and villi as well. Additionally, …
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OVEREXPRESSION OR REDUCED BIOAVAILABILITY OF VEGF DURING MOUSE POST-NATAL INTESTINAL DEVELOPMENT ALTERS THE PROLIFERATION OF INTESTINAL STEM CELL PROGENITOR CELLS
… decrease of crypts per measured length and <em>Lgr5</em> expression. On the other hand, sFLT-1 overexpressing mice had an increase in crypt depth with a decrease in villus height, proliferation in the transit-amplifying zone, crypts per measured length and reduced expression of <em>Dll1</em> and …
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FSP1/S100A4-expressing stem/progenitor cells are essential for TMJ growth and homeostasis
… menetettiin. Kondyylin pintakerroksessa Lgr5:tä ilmentävien solujen on aiemmin osoitettu luovan Wnt:tä inhiboivan ympäristön. Postnataalisen FSP1-ilmentymisen osoitettiin yhdistyvän yleisen Wnt-aktiivisuuden vähenemiseen kondyylissä. Wnt/β-kateniinin merkittävä aktivointi FSP1:tä …
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DNA METHYLATION ENTROPY AS A MEASURE OF STEM CELL REPLICATION AND AGING
… pattern in mouse colon intestinal stem cells (Lgr5-gfp+) mice from four different age groups (4,12,18, and 24-months) against nonstem cells (Lgr5-GFP-). We found that there are not many methylation changes between stem cells and nonstem cells of the same age (0% change in 4 month and +- 0.2% in …
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Stromal Fibroblasts Restrain The Rate of Colon Cancer Progression and Metastasis By Suppressing Regulatory T Cells and Colon Cancer Stem Cells
… the population of cancer stem cells expressing Lgr5 and Dclk1 was increased in αSMA+ myofibroblast-depleted tumors. In conclusion, our study reveals the unknown functional role of myofibroblasts in regulating T cell-meditated anti- tumor immunity and stemness features in colon cancer cells.</p>
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Development and Characterization of recombinant immunotoxins for cervical carcinoma
… Studies have shown that cell surface receptors LGR5, EpCAM and CD90 are overexpressed in cervical carcinoma. Full length antibodies have been proven to be less effective when used against tumours whereas, various formats of antibody such as fragment antigen binding (Fab) and single chain …
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Insights into the mechanisms of wound repair and the role of TP508 in tissue regeneration
… G protein-coupled receptor 5 positive (LGR5+) bone marrow stem cells.
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Studying the Effect of TBX4 Loss-of-Function on Postnatal Lung Development and How it Predisposes to Pulmonary Hypertension
… WNT, VEGF, and BMP signaling) and genes (Lgr5, Tnc, Wnt3a, Areg, Gdf2, and Bmper) relevant to lung alveologenesis, angiogenesis, and PH. This study contributes significant knowledge that clinicians can use to diagnose, classify, and treat patients with TBX4 mutations.
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Hepatitis C Virus-Induced Hepatocellular Carcinoma: Cancer Stem Cell and Gene Therapy
… of putative stem cell markers DCAMKL-1, Lgr5, CD133 and c-Myc. We also showed that HCV replication is severely impaired by siRNA-led depletion of the microtubule filaments (MTFs)-associated DCAMKL-1. The cholesterol-lowering drug, fluvastatin, reduces DCAMKL-1 RNA and affects its protein …
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Development Of Recombinant Immunotherapeutics for Triple-Negative Breast Cancer (TNBC)
… identified to be overexpressed in TNBC, CD90, LGR5 and EpCAM were considered for this study. Due to the limited effectiveness of full mAbs penetration into tumours, smaller antibody fragments such as scFv and Fabs have shown a greater ability to penetrate tumour tissues. These smaller antibody …
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Understanding regional diversity in the human biliary tree through transcriptomic profiling of primary tissues and in vitro derived organoids
… demonstrated expression of adult stem cell (LGR5/PROM1) and ductal (KRT19/KRT7) markers suggesting these cultures contained cells with a biliary stem/progenitor phenotype. Further, I show that IHBD organoids are distinct from EHBD organoids requiring different conditions for sustained growth. …
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