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Showing 1 to 18 of 18 for “"LCAT"”.
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Protein and cell therapy for lecithin-cholesterol acyltransferase (LCAT) deficiency
Lecithin-cholesterol acyltransferase (LCAT) is an enzyme principally secreted by the liver into the circulation where it esterifies cholesterol and plays a key role in high- density lipoprotein (HDL) metabolism. In familial and acquired (liver disease) LCAT deficiency, the failure to esterify …
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ROLE OF LECITHIN:CHOLESTEROL ACYLTRANSFERASE (LCAT) IN BRAIN CHOLESTEROL METABOLISM AND ITS INVOLVEMENT IN ALZHEIMER¿S DISEASE
… unknown. Lecithin:cholesterol acyltransferase (LCAT) is the unique enzyme responsible for the esterification of sterols in biological fluids and plays a key role in cholesterol metabolism. In the brain apoE is the main LCAT cofactor, and apoE4, one of the three apoE isoforms, is the strongest …
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Factors affecting the binding of lecithin:cholesterol acyltransferase with interfacial substrates
… by lecithin:cholesterol acyltransferase (LCAT) maintains a gradient for cholesterol diffusion from cell membranes and atherosclerotic plaques and plays a critical part in the maintenance of cholesterol homeostasis. The principal protein of HDL, apolipoprotein A-I (apoA-I) is a major …
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Effects of Glycosylation on Lecithin -Cholesterol Acyltransferase
LCAT glycosylation mutants N84Q and N384Q were examined to determine the effects of deleting individual glycan chains. Purified N84Q LCAT did not possess measurable enzymatic activity or interfacial binding affinity for rHDL. Purified N384Q was more enzymatically active than WT LCAT, but lost all …
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Study of anti-inflammatory functions of HDL in experimental mouse models
… As lecithin-cholesterol acyltransferase (LCAT) is a critical enzyme in the maturation of HDL we investigated whether LCAT-deficient (Lcat-/-) mice that lack mature HDL present an increased LPS-induced inflammatory response. The effects in Lcat-/- mice were compared to Apolipoprotein …
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Pre-Clinical Development Of Aav Mediated Gene Therapy For Familial Lecithin Cholesterol Acyltransferase Deficiency
Familial lecithin cholesterol acyltransferase (LCAT) deficiency (FLD), is a rare monogenic disease, characterized by abnormal lipid profile, corneal opacities, anemia and renal disease which progresses to renal failure. There is currently no approved treatment for patients with this disease. FLD is …
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A Learning Center Assessment Tool for the Assessment of Academic Services of a Private Learning Center
… named the Learning Center Assessment Tool (LCAT) for the assessment of academic services of a private learning center to be used by local school districts personnel. A synthesis of the literature was used to develop components of the LCAT. Superintendents from San Diego County, California …
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Probing the Structure and Function of Lecithin Cholesterol Acyltransferase by Site-Directed and Deletion Mutagenesis
… have established further relationships between LCAT and other interfacially activated lipases and have contributed to the understanding of the functional regions of LCAT.
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Apolipoprotein E Activation and Phosphatidylcholine Substrate Requirements of Lecithin Cholesterol Acyl Transferase
… of lecithin cholesterol acyl transferase (LCAT) by human apolipoprotein E (apo E) was investigated by incorporating apo E into discoidal complexes of egg phosphatidylcholine (egg-PC) and cholesterol by the cholate dialysis method. These complexes were systematically compared to apo A-I …
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The study of spherical high-density lipoproteins and the intermediates involved in their formation
… in lecithin cholesterol acyltransferase (LCAT), become spherical in morphology upon incubation with LCAT and an exogenous source of cholesterol due to the accumulation of cholesterol esters in a neutral lipid core. To date, much has been learned about the structure and function of …
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Τα συστατικά στοιχεία του συστήματος λιπιδίων και λιποπρωτεϊνών ως κεντρικοί ρυθμιστές στην εμφάνιση της παχυσαρκίας και της μη αλκοολικής λιπώδους νόσου του ήπατος σε πειραματικά μοντέλα ποντικών
… ενζύμου λεκιθινο-χοληστερολική ακυλοτρανσφεράση (LCAT). Η apoA-I αποτελεί το κύριο συστατικό των υψηλής πυκνότητας λιποπρωτεϊνών (HDL) και είναι υπεύθυνη για την σύνθεση τους, η LCAT εστεροποιεί την ελεύθερη χοληστερόλη των λιποπρωτεϊνών του πλάσματος και ευθύνεται για το σχηματισμό των ώριμων …
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Lipoprotein lipidomics in obesity-associated metabolic and hepatic disorders
… the enzyme lecithin–cholesterol acyltransferase (LCAT). Dysfunctional LCAT could partly mediate the elevated CVD risk in MetS patients. Our study demonstrated, for the first time, the link between reduced LCAT activity and plasma lipidome in MetS. In the BioNASH cohort, we observed a generalised …
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Structure and function studies on five homogeneous reconstituted HDL complexes
… with lecithin cholesterol acyltransferase (LCAT) was also investigated. The rHDL structures were investigated by assays for chemical composition; and the dimensions were determined by native gel electrophoresis and electron microscopy. The protein structure was studied by a variety of …
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Factors Influencing the Formation and Distribution of Cholesterol Esters in Human Plasma
… the lecithin-cholesterol acyltransferase (LCAT) and lipid transfer protein (LTP) reactions in plasma. These two plasma proteins play important roles in determining the formation and distribution of cholesterol esters in plasma.
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A Characterization of the Effect of Apolipoprotein AII on the Structure and Function of Apolipoprotein AI in Reconstituted High Density Lipoproteins
… binding. The addition of apoAII to AIrHDL and LCAT resulted in a decrease in apparent $\lambda$max/Km compared to the control AIrHDL and LCAT. The data show that the binding of apoAII to AIrHDL destabilizes apoAI structure on rHDL. The presence of apoAII also affects the function of apoAI by …
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Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
… similar fluorescence properties and activated LCAT equally well. In conclusion, proapoA-I expressed and purified from E. coli is functionally and structurally indistinguishable from apoA-I purified from plasma when analyzed in vitro. Several proapoA-I mutants were constructed, purified and …
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Roles of human plasma phospholipid transfer protein in high-density lipoprotein metabolism
… of plasma HDL was enhanced in the presence of LCAT, and also LDL and VLDL.
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Biochemical markers of hypertension and chronic renal failure in a Gulf Arab population of the United Arab Emirates
… of lecithin: cholesterol acyltransferase (LCAT), has been documented and this has been shown to be associated with low levels of HDLcholesterol and other lipid changes including membrane lipid composition. in untreated hypertensives, low I-IDL-cholesterol have also been documented, but …