Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"LC3B"”.
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Hsp27 and Chemotherapy-Induced Autophagy As Biomarkers In Osteosarcoma
… We evaluated the occurrence of cytoplasmic LC3B (light chain 3B)-positive puncta (a marker of autophagy) and presence of HSP27 (heat shock protein 27) in cancer cells within pre-treatment biopsy, post-treatment surgical resection, and metastatic osteosarcoma specimens by immunohistochemistry …
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Scalable methods for the discovery of autophagy and disease genes
… assays: (1) an autophagic flux assay, SRAI-LC3B; and (2) an assay for levels of the ATG12-ATG5 conjugate, an important early autophagy marker. SRAI-LC3B can be used to assess autophagic flux via flow cytometry or microscopy and responds robustly to established pharmacological and genetic …
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DESIGN AND DEVELOPMENT OF RNA-BASED DRUGS USING MOLECULAR DYNAMICS SIMULATIONS AND ARTIFICIAL INTELLIGENCE METHODS
… inhibition was pursued by targeting the LC3B RNA-binding motif using novel Peptide Nucleic Acids (PNAs) to disrupt cancer cell survival mechanisms. Second, XNA sequences were optimized to target the DNA-binding domain of the oncogenic protein HMGB1. Third, aptamers were designed to bind …
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Autophagy: activation, function and regulation by a protein restricted diet during pregnancy and lactation
… has been reported that HDAC3 inhibition induces LC3B expression, a primary autophagy marker and lipid accumulation. More importantly, we show that amino acid limitation induces autophagy through HDAC3-dependent expression of LC3B and its increased association with lipid droplets in a hepatic cell …
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Investigating systemic effects of chronic stress on hepatic and cardiac tissue in female Wistar rats
… fractions. Autophagy-related proteins (p62, LC3B, Beclin-1, SNAP29, SOD1/2) were quantified by Western blotting. CRS induced mild oxidative damage restricted to cardiac tissue, with reduced SOD activity and elevated compartmental malondialdehyde, while hepatic redox parameters remained …
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Analysis of markers of cell division cycle, apoptosis and autophagy flux in glioblastoma
… caspase 3. Markers of autophagy flux included LC3B, a ubiquitin like protein that form part of the core autophagy machinery; and p62, a mammalian autophagy receptor that binds ubiquitinated proteins. A total of 66 patients were recruited to the study between 2007 and 2009. Data were collected …
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Directed Evolution of Macrocyclic Peptides For Inhibition of Autophagy
… LC3. The resulting peptides bound LC3A and LC3B—two essential components of the autophagosome maturation machinery—with mid-nanomolar affinities and disrupted protein-protein interactions (PPIs) between LC3 and its binding partners in vitro. LC3-binding SUPR peptides re-sensitized …
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Probing the protein homeostasis mechanisms in long-lived naked mole-rats
… conditions of mild ER stress. In Chapter 5, LC3B turnover and transcriptional changes of autophagy markers under rapamycin (RA) and chloroquine (CQ)-treated conditions were monitored in an NMR skin fibroblast cell line, where the sensitivity of the NMR skin fibroblasts to CQ, when compared to …
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Endosomal Sorting Complex Required for Transport (ESCRT): Investigation of the expression and functional roles in neurodegenerative diseases
… including altered autophagy markers such as LC3B and p62. Finally, knockout of SOCS3 enhances autophagy via TBK1 stabilisation. Conclusion: alterations in the expression of ESCRT proteins and SOCS3 may have a role in the pathogenesis of MND and could be new therapeutic targets for treating …
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A maternal low-protein diet during gestation induces hepatic autophagy gene expression only in the female offspring Sprague-Dawley rats
… expression of hepatic autophagy gene including LC3b, P62/SQSTM1, Beclin1, Atg3, Atg7, Atg5, and Atg10 was increased significantly only in the female offspring by gestational low-protein diet. The induction of gene expression was consistent with the activated eIF2a/ATF4 pathway in female …
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Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
… marker LC3. Bioinformatics showed how MAP1LC3B (LC3B gene) can be highly expressed in tumours associated with poor disease outcome. Drug-treated OS cell lines showed a remarkable increase in LC3 puncta (autophagy marker) detected by Immunofluorescence. Additionally, drug-treated OS cell …
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Parallels and Divergences in Multisystem Proteinopathy Genes: Stress Granules, Autophagy, and Myogenic Deficits
… commonalities which fall into two categories: LC3B-intracting domain containing autophagy adaptors (SQSTM1, VCP, OPTN) and prion-like domain containing stress granule proteins (HNRNPA2B1, HNRNPA1, MATR3, TIA1). Previous studies identified that a non-pathogenic variant of the non-classical MSP …
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The early time course of autophagy in human peripheral blood mononuclear cells following endurance exercise
… 1h post-exercise when compared to pre-exercise. LC3b decreased following MIE at 1h (p < 0.01) and was increased at 2h, post-exercise when compared to baseline. HSPA1A was decreased at 1h following HIE, when compared to baseline, p < 0.01. HSP70 and LC3-II were moderately and significantly related …
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TRIB3 as an integrator of metabolic stresses: Role in lung cancer progression
… overexpression also led to downregulation of LC3B and other autophagy markers while increasing the expression of apoptotic genes. Our study reveals complex roles of TRIB3 in lung cancer progression. Induced by metabolic stresses, TRIB3 blocked cell cycle progression through the upregulation of …
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MITOCHONDRIAL DNA COPY NUMBER AND AUTOPHAGY IN THE AGING BRAIN AND IN AN ALZHEIMER MOUSE MODEL
… expressing the dual fusion protein, mCherry-GFP-LC3B. This system enables the distinction of early (autophagosomal formation) from late (autophagosomal degradation) stages of autophagy. Autophagosomal formation increased in female AD-Tg mice, while male AD-Tg neurons have decreased formation of …
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Investigating the role of the UDP-galactose transporter SLC35A2 in the regulation of HIF signalling
… showed elevated levels of the autophagy marker LC3B. Finally, from analyses of the TCGA-KIRC database I found *SCL35A2* expression was higher in patients with non-mutant VHL versus mutant VHL in renal cancers. In support of these findings, I showed that basal SLC35A2 protein and mRNA levels were …